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Proteome Profiler Human Cytokine Array Kit

Catalog #: ARY005B
288 Citations 12 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 36 different cytokine antibodies
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ARY005B
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The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
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Citations (288)
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Proteome Profiler Human Cytokine Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk.

Troubleshooting Guide

Key Benefits

  • Detects 36 human cytokines, chemokines, and acute phase proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.

Principle of the Assay

The Proteome Profiler Human Cytokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • Human Cytokine Array, Panel A nitrocellulose membranes spotted with 36 different antibodies to human cytokines, chemokines, and acute phase proteins
  • Array Buffer 4
  • Array Buffer 5
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

 
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
C5a IL-4 IL-27
CD40 Ligand IL-5 IL-32 alpha
G-CSF IL-6 CXCL10/IP-10
GM-CSF IL-8 CXCL11/I-TAC
CXCL1/GRO alpha IL-10 CCL2/MCP-1
CCL1/I-309 IL-12 p70 MIF
ICAM-1 IL-13 MIP-1 alpha/MIP-1 beta
IFN-gamma IL-16 CCL5/RANTES
IL-1 alpha IL-17 CXCL12/SDF-1
IL-1 beta IL-17E Serpin E1/PAI-1
IL-1ra IL-18 TNF-alpha
IL-2 IL-21 TREM-1

Assays for Analytes represented in the Human Cytokine Array Kit

Analyte Quantikine® ELISA Kits Quantikine® HS (High Sensitivity) ELISA Kits QuantiGlo® ELISA Kits DuoSet® ELISA Development Systems
C5a       DY2037
CD40 Ligand DCDL40     DY617
G-CSF DCS50 HSTCS0   DY214
GM-CSF DGM00 HSGM0   DY215
CXCL1/GRO alpha DGR00     DY275
CCL1/I-309       DY272
ICAM-1 DCIM00     DY720
IFN-gamma DIF50     DY285
IL-1 alpha DLA50     DY200
IL-1 beta DLB50 HSLB00C QLB00B DY201
IL-1ra DRA00B     DY280
IL-2 D2050   Q2000B DY202
IL-4 D4050 HS400   DY204
IL-5 D5000B     DY205
IL-6 D6050 HS600B Q6000B DY206
CXCL8/IL-8 D8000C HS800 Q8000B DY208
IL-10 D1000B HS100C Q1000 DY217B
IL-12 p70 D1200 HS120   DY1270
IL-13 D1300B     DY213
IL-16 D1600     DY316
IL-17 D1700     DY317
IL-17E        
IL-18 7620      
IL-21        
IL-27      

DY2526
IL-32 alpha        
CXCL10/IP-10 DIP100     DY266
CXCL11/I-TAC DCX110     DY672
CCL2/MCP-1 DCP00     DY279
MIF DMF00B     DY289
CCL3/MIP-1 alpha DMA00     DY270
CCL4/MIP-1 beta DMB00     DY271
Serpin E1/PAI-1 DSE100, DTSE100    

DY1786
CCL5/RANTES DRN00B     DY278
CXCL12/SDF-1 DSA00     DY350
TNF-alpha DTA00C HSTA00D QTA00B DY210
TREM-1 DTRM10B     DY1278B

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

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Scientific Data

N/A The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. View Larger

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. The amount of cell culture supernate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 1A: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL LPS for 4 hours, then 5mM Nigericin (Tocris, Catalog # 4312) for 1 hour (50 µL of cell culture supernate, 10 minute exposure). 1B: SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (500 µL of cell culture supernate, 10 minute exposure).

N/A The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell lysates. View Larger

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell lysates. The amount of cell lysate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 2A: SW1353 human chondrosarcoma cells were untreated or treated with 5 ng/mL recombinant human IL-1 alpha (R&D Systems, Catalog # 200-LA) and 10 ng/mL recombinant human OSM (R&D Systems, Catalog # 295-OM) for 24 hours (200 µg of cell lysate, 5 minute exposure). 2B: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, then 1 µg/mL LPS for 16 hours (200 µg of cell lysate, 5 minute exposure).

N/A The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in tissue lysate samples. View Larger

The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in tissue lysate samples. 200 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.

1. ICAM-1/CD54
2. MIF
3. Complment Component C5/C5a
4. CXCL1/GRO alpha
5. IL-8
6. IL-16
7. IL-18
8. CCL5/RANTES
9. IL-1ra/IL-1F3
10. Serpin E1/PAI-1

Citations for Proteome Profiler Human Cytokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

288 Citations: Showing 1 - 10
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  1. Oncogenic KEAP1 mutations activate TRAF2-NF?B signaling to prevent apoptosis in lung cancer cells
    Authors: Deen, AJ;Adinolfi, S;Härkönen, J;Patinen, T;Liu, X;Laitinen, T;Takabe, P;Kainulainen, K;Pasonen-Seppänen, S;Gawriyski, LM;Arasu, UT;Selvarajan, I;Mäkinen, P;Laitinen, H;Kansanen, E;Kaikkonen, MU;Poso, A;Varjosalo, M;Levonen, AL;
    Redox biology  2024-02-01
  2. iPSC-derived PSEN2 (N141I) astrocytes and microglia exhibit a primed inflammatory phenotype
    Authors: Sullivan, MA;Lane, SD;McKenzie, ADJ;Ball, SR;Sunde, M;Neely, GG;Moreno, CL;Maximova, A;Werry, EL;Kassiou, M;
    Journal of neuroinflammation  2024-01-04
  3. Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation
    Authors: Hong, J;Son, M;Sin, J;Kim, H;Chung, DK;
    Nutrients  2023-11-09
  4. Mesenchymal stem cell-derived secretomes-enriched alginate/ extracellular matrix hydrogel patch accelerates skin wound healing
    Authors: Kwon, JW;Savitri, C;An, B;Yang, SW;Park, K;
    Biomaterials research  2023-10-31
  5. Development, Characterization and Pharmacological Evaluation of Cannabidiol-Loaded Long Circulating Niosomes
    Authors: Gugleva, V;Ahchiyska, K;Georgieva, D;Mihaylova, R;Konstantinov, S;Dimitrov, E;Toncheva-Moncheva, N;Rangelov, S;Forys, A;Trzebicka, B;Momekova, D;
    Pharmaceutics  2023-10-03
  6. Longitudinal changes in tear cytokines and antimicrobial proteins in trachomatous disease
    Authors: Barton, A;Faal, N;Ramadhani, A;Derrick, T;Mafuru, E;Mtuy, T;Massae, P;Malissa, A;Joof, H;Makalo, P;Sillah, A;Harte, A;Pickering, H;Bailey, R;Mabey, DC;Burton, MJ;Holland, MJ;
    PLoS neglected tropical diseases  2023-10-01
  7. Regulatory role of human endometrial gland secretome on macrophage differentiation
    Authors: Lin, L;Bai, K;Li, J;Chiu, PCN;Lee, CL;
    Journal of reproductive immunology  2023-09-30
  8. New JAK3-INSL3 Fusion Transcript-An Oncogenic Event in Cutaneous T-Cell Lymphoma
    Authors: Velatooru, LR;Hu, CH;Bijani, P;Wang, X;Bojaxhi, P;Chen, H;Duvic, M;Ni, X;
    Cells  2023-09-29
  9. Vascular smooth muscle cells enhance immune/vascular interplay in a 3-cell model of vascular inflammation
    Authors: Wiejak, J;Murphy, FA;Maffia, P;Yarwood, SJ;
    Scientific reports  2023-09-23
  10. Head and Neck Squamous Cell Carcinoma Biopsies Maintained Ex Vivo on a Perfusion Device Show Gene Changes with Time and Clinically Relevant Doses of Irradiation
    Authors: Green, V;Baldwin, L;England, J;Marshall, G;Frost, L;Moore, C;Greenman, J;
    Cancers  2023-09-15
  11. FBXO21 mediated degradation of p85? regulates proliferation and survival of acute myeloid leukemia
    Authors: Dobish, KK;Wittorf, KJ;Swenson, SA;Bean, DC;Gavile, CM;Woods, NT;Ghosal, G;Hyde, RK;Buckley, SM;
    Leukemia  2023-09-09
  12. Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53
    Authors: Lee, JD;Menasche, BL;Mavrikaki, M;Uyemura, MM;Hong, SM;Kozlova, N;Wei, J;Alfajaro, MM;Filler, RB;Müller, A;Saxena, T;Posey, RR;Cheung, P;Muranen, T;Heng, YJ;Paulo, JA;Wilen, CB;Slack, FJ;
    bioRxiv : the preprint server for biology  2023-09-01
  13. Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation
    Authors: Shen, CJ;Chan, RH;Lin, BW;Li, NC;Huang, YH;Chang, WC;Chen, BK;
    Theranostics  2023-08-21
  14. Macrophage Profiling in Head and Neck Cancer to Improve Patient Prognosis and Assessment of Cancer Cell-Macrophage Interactions Using Three-Dimensional Coculture Models
    Authors: Mhaidly, N;Journe, F;Najem, A;Stock, L;Trelcat, A;Dequanter, D;Saussez, S;Descamps, G;
    International journal of molecular sciences  2023-08-15
  15. Mechanism and therapeutic implications of pomalidomide-induced immune surface marker upregulation in EBV-positive lymphomas
    Authors: Jaeger, HK;Davis, DA;Nair, A;Shrestha, P;Stream, A;Yaparla, A;Yarchoan, R;
    Scientific reports  2023-07-18
  16. Proteomics and phosphoproteomics profiling in glutamatergic neurons and microglia in an iPSC model of Jansen de Vries Syndrome
    Authors: Aguilan, JT;Pedrosa, E;Dolstra, H;Baykara, RN;Barnes, J;Zhang, J;Sidoli, S;Lachman, HM;
    bioRxiv : the preprint server for biology  2023-07-08
  17. The role of pneumococcal extracellular vesicles on the pathophysiology of the kidney disease hemolytic uremic syndrome
    Authors: Battista, M;Hoffmann, B;Bachelot, Y;Zimmermann, L;Teuber, L;Jost, A;Linde, S;Westermann, M;Müller, MM;Slevogt, H;Hammerschmidt, S;Figge, MT;Vilhena, C;Zipfel, PF;
    mSphere  2023-06-26
  18. Tanshinone IIA and Cryptotanshinone Counteract Inflammation by Regulating Gene and miRNA Expression in Human SGBS Adipocytes
    Authors: Carpi, S;Quarta, S;Doccini, S;Saviano, A;Marigliano, N;Polini, B;Massaro, M;Carluccio, MA;Calabriso, N;Wabitsch, M;Santorelli, FM;Cecchini, M;Maione, F;Nieri, P;Scoditti, E;
    Biomolecules  2023-06-23
  19. Matrix Metalloproteinase 9 Induced in Esophageal Squamous Cell Carcinoma Cells via Close Contact with Tumor-Associated Macrophages Contributes to Cancer Progression and Poor Prognosis
    Authors: Tsukamoto, S;Koma, YI;Kitamura, Y;Tanigawa, K;Azumi, Y;Miyako, S;Urakami, S;Hosono, M;Kodama, T;Nishio, M;Shigeoka, M;Yokozaki, H;
    Cancers  2023-05-30
  20. Regulation of Cancer Stem Cells and Epithelial-Mesenchymal Transition by CTNNAL1 in Lung Cancer and Glioblastoma
    Authors: Kahm, YJ;Jung, U;Kim, RK;
    Biomedicines  2023-05-17
  21. Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1
    Authors: Omar, FA;Brown, TC;Gillanders, WE;Fleming, TP;Smith, MA;Bremner, RM;Sankpal, NV;
    PloS one  2023-05-16
  22. Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
    Authors: Yndart Arias, A;Kolishetti, N;Vashist, A;Madepalli, L;Llaguno, L;Nair, M;
    Scientific reports  2023-05-05
  23. Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling
    Authors: Bian, F;Lan, YW;Zhao, S;Deng, Z;Shukla, S;Acharya, A;Donovan, J;Le, T;Milewski, D;Bacchetta, M;Hozain, AE;Tipograf, Y;Chen, YW;Xu, Y;Shi, D;Kalinichenko, VV;Kalin, TV;
    Nature communications  2023-05-04
  24. Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer
    Authors: S Mukhopadhy, J Encarnació, EY Lin, ASW Sohn, H Zhang, JD Mancias, AC Kimmelman
    Science Advances, 2023-04-19;9(16):eadf9284.  2023-04-19
  25. Constitutive secretion of pro-IL-18 allows keratinocytes to initiate inflammation during bacterial infection
    Authors: AF Johnson, JS Sands, KM Trivedi, R Russell, DL LaRock, CN LaRock
    PloS Pathogens, 2023-04-17;19(4):e1011321.  2023-04-17
  26. Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response
    Authors: CY Lin, KY Huang, SH Kao, MS Lin, CC Lin, SC Yang, WC Chung, YH Chang, RJ Chein, PC Yang
    Science Advances, 2023-04-07;9(14):eade9944.  2023-04-07
  27. Cancer-associated mesothelial cell-derived ANGPTL4 and STC1 promote the early steps of ovarian cancer metastasis
    Authors: P Bajwa, K Kordylewic, A Bilecz, RR Lastra, K Wroblewski, Y Rinkevich, E Lengyel, HA Kenny
    JCI Insight, 2023-03-22;0(0):.  2023-03-22
  28. The early neutrophil-committed progenitors aberrantly differentiate into immunoregulatory monocytes during emergency myelopoiesis
    Authors: N Ikeda, H Kubota, R Suzuki, M Morita, A Yoshimura, Y Osada, K Kishida, D Kitamura, A Iwata, S Yotsumoto, D Kurotaki, K Nishimura, A Nishiyama, T Tamura, T Kamatani, T Tsunoda, M Murakawa, Y Asahina, Y Hayashi, H Harada, Y Harada, A Yokota, H Hirai, T Seki, M Kuwahara, M Yamashita, S Shichino, M Tanaka, K Asano
    Cell Reports, 2023-02-28;42(3):112165.  2023-02-28
  29. Cyanobacterial Harmful Bloom Lipopolysaccharides Induce Pro-Inflammatory Effects in Immune and Intestinal Epithelial Cells In Vitro
    Authors: V Sko?ková, O Vaší?ek, E Sychrová, I Sovadinová, P Babica, L Šindlerová
    Toxins, 2023-02-21;15(3):.  2023-02-21
  30. Vitamin D (1alpha,25(OH)2D3) supplementation minimized multinucleated giant cells formation and inflammatory response during Burkholderia pseudomallei infection in human lung epithelial cells
    Authors: P Mattrasong, S Wongkaewkh, S Taweechais, S Chareonsud, T Techawiwat, T Ngamsiri, S Kanthawong
    PLoS ONE, 2023-02-09;18(2):e0280944.  2023-02-09
  31. The D405N Mutation in the Spike Protein of SARS-CoV-2 Omicron BA.5 Inhibits Spike/Integrins Interaction and Viral Infection of Human Lung Microvascular Endothelial Cells
    Authors: A Bugatti, F Filippini, S Messali, M Giovanetti, C Ravelli, A Zani, M Ciccozzi, A Caruso, F Caccuri
    Viruses, 2023-01-24;15(2):.  2023-01-24
  32. Radiofrequency Ablation Remodels the Tumor Microenvironment and Promotes Neutrophil-Mediated Abscopal Immunomodulation in Pancreatic Cancer
    Authors: EY Faraoni, BJ O'Brien, LN Strickland, BK Osborn, V Mota, J Chaney, CL Atkins, P Cen, J Rowe, J Cardenas, KL Poulsen, CJ Wray, NC Thosani, JM Bailey-Lun
    Cancer Immunology Research, 2023-01-03;11(1):4-12.  2023-01-03
  33. EGFR-Tyrosine Kinase Inhibitors Induced Activation of the Autocrine CXCL10/CXCR3 Pathway through Crosstalk between the Tumor and the Microenvironment in EGFR-Mutant Lung Cancer
    Authors: SH Hong, N Kang, O Kim, SA Hong, J Park, J Kim, MA Lee, J Kang
    Cancers, 2022-12-25;15(1):.  2022-12-25
  34. Inhibition of triple negative breast cancer-associated inflammation and progression by N- acylethanolamine acid amide hydrolase (NAAA)
    Authors: O Benchama, MS Malamas, K Praveen, EC Ethier, MK Williams, A Makriyanni, HK Avraham
    Scientific Reports, 2022-12-23;12(1):22255.  2022-12-23
  35. Clozapine Suppresses the Gene Expression and the Production of Cytokines and Up-Regulates Cyclooxygenase 2 mRNA in Human Astroglial Cells
    Authors: Y Yuhas, S Ashkenazi, E Berent, A Weizman
    Brain sciences, 2022-12-12;12(12):.  2022-12-12
  36. Non-small cell lung cancer-derived exosomes promote proliferation, phagocytosis, and secretion of microglia via exosomal microRNA in the metastatic microenvironment
    Authors: P Chen, Y Li, R Liu, Y Xie, Y Jin, M Wang, Z Yu, W Wang, X Luo
    Translational Oncology, 2022-12-01;27(0):101594.  2022-12-01
  37. Successful targeting of PD-1/PD-L1 with chimeric antigen receptor-natural killer cells and nivolumab in a humanized mouse cancer model
    Authors: WN Liu, WY So, SL Harden, SY Fong, MXY Wong, WWS Tan, SY Tan, JKL Ong, R Rajarethin, M Liu, JY Cheng, L Suteja, JPS Yeong, NG Iyer, DW Lim, Q Chen
    Science Advances, 2022-11-23;8(47):eadd1187.  2022-11-23
  38. Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility
    Authors: C Villarroya, A Osorio, R Torres-Rui, D Gómez-Sánc, M Trakala, A Sánchez-Be, F Mercadillo, B Hurtado, B Pitarch, A Hernández-, A Gómez-Catu, D Rueda, J Perea, S Rodríguez-, M Malumbres, M Urioste
    Science Advances, 2022-11-02;8(44):eabq5914.  2022-11-02
  39. Metabolic control of CD47 expression through LAT2-mediated amino acid uptake promotes tumor immune evasion
    Authors: Z Wang, B Li, S Li, W Lin, Z Wang, S Wang, W Chen, W Shi, T Chen, H Zhou, E Yinwang, W Zhang, H Mou, X Chai, J Zhang, Z Lu, Z Ye
    Nature Communications, 2022-10-23;13(1):6308.  2022-10-23
  40. Profound Effects of Dexamethasone on the Immunological State, Synthesis and Secretion Capacity of Human Testicular Peritubular Cells
    Authors: YK Stepanov, JD Speidel, C Herrmann, N Schmid, R Behr, FM Köhn, JB Stöckl, U Pickl, M Trottmann, T Fröhlich, A Mayerhofer, H Welter
    Cells, 2022-10-09;11(19):.  2022-10-09
  41. Immune activation is essential for the antitumor activity of EZH2 inhibition in urothelial carcinoma
    Authors: A Piunti, K Meghani, Y Yu, AG Robertson, JR Podojil, KA McLaughlin, Z You, D Fantini, M Chiang, Y Luo, L Wang, N Heyen, J Qian, SD Miller, A Shilatifar, JJ Meeks
    Science Advances, 2022-10-05;8(40):eabo8043.  2022-10-05
  42. Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation
    Authors: F Caso, A Saviano, M Tasso, F Raucci, N Marigliano, S Passavanti, P Frallonard, R Ramonda, V Brancaleon, M Bucci, R Scarpa, L Costa, F Maione
    Autoimmunity reviews, 2022-10-01;21(12):103207.  2022-10-01
  43. Cytokine profile of human limbal myofibroblasts: Key players in corneal antiviral response
    Authors: A Domínguez-, Y Garfias
    Cytokine, 2022-09-29;160(0):156047.  2022-09-29
  44. The lysosomal proteome of senescent cells contributes to the senescence secretome
    Authors: M Rovira, R Sereda, D Pladevall-, V Ramponi, I Marin, M Maus, J Madrigal-M, A Díaz, F García, J Muñoz, AM Cuervo, M Serrano
    Aging Cell, 2022-09-10;0(0):e13707.  2022-09-10
  45. Obstructive Sleep Apnea Syndrome In Vitro Model: Controlled Intermittent Hypoxia Stimulation of Human Stem Cells-Derived Cardiomyocytes
    Authors: D Regev, S Etzion, H Haddad, J Gopas, A Goldbart
    International Journal of Molecular Sciences, 2022-09-07;23(18):.  2022-09-07
  46. Exosomal B7-H4 from irradiated glioblastoma cells contributes to increase FoxP3 expression of differentiating Th1 cells and promotes tumor growth
    Authors: Y Tian, C Liu, Z Li, M Ai, B Wang, K Du, W Liu, H Wang, P Yu, C Chen, J Lin, A Xu, R Li, W Zhang, Y Yuan
    Redox Biology, 2022-08-27;56(0):102454.  2022-08-27
  47. Detection of Leptomonas seymouri narna-like virus in serum samples of visceral leishmaniasis patients and its possible role in disease pathogenesis
    Authors: S Sukla, H Nath, M Kamran, SA Ejazi, N Ali, P Das, V Ravichandi, S Roy, S Biswas
    Scientific Reports, 2022-08-24;12(1):14436.  2022-08-24
  48. A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states
    Authors: NM Dräger, SM Sattler, CT Huang, OM Teter, K Leng, SH Hashemi, J Hong, G Aviles, CD Clelland, L Zhan, JC Udeochu, L Kodama, AB Singleton, MA Nalls, J Ichida, ME Ward, F Faghri, L Gan, M Kampmann
    Nature Neuroscience, 2022-08-11;0(0):.  2022-08-11
  49. Interplay of Helicobacter pylori, fibroblasts, and cancer cells induces fibroblast activation and serpin E1 expression by cancer cells to promote gastric tumorigenesis
    Authors: X Chen, W Chen, Y Zhao, Q Wang, W Wang, Y Xiang, H Yuan, Y Xie, J Zhou
    Journal of Translational Medicine, 2022-07-21;20(1):322.  2022-07-21
  50. Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling
    Authors: A Emam, X Wu, S Xu, L Wang, S Liu, B Wang
    Nature Cell Biology, 2022-07-11;24(7):1154-1164.  2022-07-11
  51. MIF/CXCR4 signaling axis contributes to survival, invasion, and drug resistance of metastatic neuroblastoma cells in the bone marrow microenvironment
    Authors: L Garcia-Ger, M García, A Garrido-Ga, S Gómez-Gonz, M Torrebadel, E Prada, G Pascual-Pa, O Muñoz, S Perez-Jaum, I Lemos, N Salvador, M Vila-Ubach, A Doncel-Req, M Suñol, AM Carcaboso, J Mora, C Lavarino
    BMC Cancer, 2022-06-17;22(1):669.  2022-06-17
  52. PRRX1 is a master transcription factor of stromal fibroblasts for myofibroblastic lineage progression
    Authors: KW Lee, SY Yeo, JR Gong, OJ Koo, I Sohn, WY Lee, HC Kim, SH Yun, YB Cho, MA Choi, S An, J Kim, CO Sung, KH Cho, SH Kim
    Nature Communications, 2022-05-19;13(1):2793.  2022-05-19
  53. Paracrine Interaction of Cholangiocellular Carcinoma with Cancer-Associated Fibroblasts and Schwann Cells Impact Cell Migration
    Authors: JP Gundlach, J Kerber, A Hendricks, A Bernsmeier, C Halske, C Röder, T Becker, C Röcken, F Braun, S Sebens, N Heits
    Journal of Clinical Medicine, 2022-05-15;11(10):.  2022-05-15
  54. Alpha-Linolenic Acid Modulates T Cell Incorporation in a 3D Tissue-Engineered Psoriatic Skin Model
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

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Proteome Profiler Human Cytokine Array Kit
By Anonymous on 08/20/2023

the provided protocol is straightforward.

Proteome Profiler Human Cytokine Array Kit
By Anonymous on 09/29/2021

Excellent results

Proteome Profiler Human Cytokine Array Kit
By Anonymous on 06/02/2021
Proteome Profiler Human Cytokine Array Kit
By Anonymous on 01/15/2021
Proteome Profiler Human Cytokine Array Kit ARY005B
Proteome Profiler Human Cytokine Array Kit
By Anonymous on 01/31/2020
Proteome Profiler Human Cytokine Array Kit ARY005B
Proteome Profiler Human Cytokine Array Kit
By jaime llc on 04/05/2019
Proteome Profiler Human Cytokine Array Kit ARY005B
Proteome Profiler Human Cytokine Array Kit
By Anonymous on 03/13/2019
Proteome Profiler Human Cytokine Array Kit
By Anonymous on 05/04/2018
Proteome Profiler Human Cytokine Array Kit
By Anonymous on 04/17/2018
Proteome Profiler Human Cytokine Array Kit
By Anonymous on 02/13/2018
Proteome Profiler Human Cytokine Array Kit ARY005B
Proteome Profiler Human Cytokine Array Kit
By Adam Guess on 10/20/2017
Proteome Profiler Human Cytokine Array Kit ARY005B
Proteome Profiler Human Cytokine Array Kit
By Farwah Iqbal on 01/25/2017

Tested condition media of MSCs from the umbilical cord co-cultured with NK cells. The goal of this experiments was to detect chages in inflammatory factors in the system when MSCs were cultured with NK cells. The lack of detected secreted proteins is promising because this suggests that MSCs do not up-regulate large inflammatory proteins following immune cell activation.

Left image : MSC cultured in RPMI media (Control)
Right image: MSC co-cultured with NK cells in same RPMI media

Proteome Profiler Human Cytokine Array Kit ARY005B