Proteome Profiler Human Cytokine Array Kit
Proteome Profiler Human Cytokine Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk.
Key Benefits
- Detects 36 human cytokines, chemokines, and acute phase proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Human Cytokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- Human Cytokine Array, Panel A nitrocellulose membranes spotted with 36 different antibodies to human cytokines, chemokines, and acute phase proteins
- Array Buffer 4
- Array Buffer 5
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Human Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
C5a | IL-4 | IL-27 |
CD40 Ligand | IL-5 | IL-32 alpha |
G-CSF | IL-6 | CXCL10/IP-10 |
GM-CSF | IL-8 | CXCL11/I-TAC |
CXCL1/GRO alpha | IL-10 | CCL2/MCP-1 |
CCL1/I-309 | IL-12 p70 | MIF |
ICAM-1 | IL-13 | MIP-1 alpha/MIP-1 beta |
IFN-gamma | IL-16 | CCL5/RANTES |
IL-1 alpha | IL-17 | CXCL12/SDF-1 |
IL-1 beta | IL-17E | Serpin E1/PAI-1 |
IL-1ra | IL-18 | TNF-alpha |
IL-2 | IL-21 | TREM-1 |
Assays for Analytes represented in the Human Cytokine Array Kit
Analyte | Quantikine® ELISA Kits | Quantikine® HS (High Sensitivity) ELISA Kits | QuantiGlo® ELISA Kits | DuoSet® ELISA Development Systems |
C5a | DY2037 | |||
CD40 Ligand | DCDL40 | DY617 | ||
G-CSF | DCS50 | HSTCS0 | DY214 | |
GM-CSF | DGM00 | HSGM0 | DY215 | |
CXCL1/GRO alpha | DGR00 | DY275 | ||
CCL1/I-309 | DY272 | |||
ICAM-1 | DCIM00 | DY720 | ||
IFN-gamma | DIF50C | DY285B | ||
IL-1 alpha | DLA50 | DY200 | ||
IL-1 beta | DLB50 | HSLB00C | QLB00B | DY201 |
IL-1ra | DRA00B | DY280 | ||
IL-2 | D2050 | Q2000B | DY202 | |
IL-4 | D4050 | HS400 | DY204 | |
IL-5 | D5000B | DY205 | ||
IL-6 | D6050B | HS600B | Q6000B | DY206 |
CXCL8/IL-8 | D8000C | HS800 | Q8000B | DY208 |
IL-10 | D1000B | HS100C | Q1000 | DY217B |
IL-12 p70 | D1200 | HS120 | DY1270 | |
IL-13 | D1300B | DY213 | ||
IL-16 | D1600 | DY316 | ||
IL-17 | D1700 | DY317 | ||
IL-17E | ||||
IL-18 | 7620 | |||
IL-21 | ||||
IL-27 | ||||
IL-32 alpha | ||||
CXCL10/IP-10 | DIP100 | DY266 | ||
CXCL11/I-TAC | DCX110 | DY672 | ||
CCL2/MCP-1 | DCP00 | DY279 | ||
MIF | DMF00B | DY289 | ||
CCL3/MIP-1 alpha | DMA00 | DY270 | ||
CCL4/MIP-1 beta | DMB00 | DY271 | ||
Serpin E1/PAI-1 | DSE100, DTSE100 | |||
CCL5/RANTES | DRN00B | DY278 | ||
CXCL12/SDF-1 | DSA00 | DY350 | ||
TNF-alpha | DTA00C | HSTA00D | QTA00B | DY210 |
TREM-1 | DTRM10B | DY1278B |
Specifications
Product Datasheets
Scientific Data
The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. The amount of cell culture supernate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 1A: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL LPS for 4 hours, then 5mM Nigericin (Tocris, Catalog # 4312) for 1 hour (50 µL of cell culture supernate, 10 minute exposure). 1B: SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (500 µL of cell culture supernate, 10 minute exposure).
The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell lysates. The amount of cell lysate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 2A: SW1353 human chondrosarcoma cells were untreated or treated with 5 ng/mL recombinant human IL-1 alpha (R&D Systems, Catalog # 200-LA) and 10 ng/mL recombinant human OSM (R&D Systems, Catalog # 295-OM) for 24 hours (200 µg of cell lysate, 5 minute exposure). 2B: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, then 1 µg/mL LPS for 16 hours (200 µg of cell lysate, 5 minute exposure).
The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in tissue lysate samples.
200 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.
1. ICAM-1/CD54
2. MIF
3. Complment Component C5/C5a
4. CXCL1/GRO alpha
5. IL-8
6. IL-16
7. IL-18
8. CCL5/RANTES
9. IL-1ra/IL-1F3
10. Serpin E1/PAI-1
Citations for Proteome Profiler Human Cytokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Influence of Anticoagulants and Heparin Contaminants on the Suitability of MMP-9 as a Blood-Derived Biomarker
Authors: Küper, D;Klos, J;Kühl, F;Attili, R;Brand, K;Weissenborn, K;Lichtinghagen, R;Huber, R;
International journal of molecular sciences 2024-09-20
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Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population
Authors: Meshko, B;Volatier, TLA;Mann, J;Kluth, MA;Ganss, C;Frank, MH;Frank, NY;Ksander, BR;Cursiefen, C;Notara, M;
International journal of molecular sciences 2024-09-07
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Differential effects of TLR3 and TLR4 activation on MSC-mediated immune regulation
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Biochemistry and biophysics reports 2024-09-01
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The MondoA-dependent TXNIP/GDF15 axis predicts oxaliplatin response in colorectal adenocarcinomas
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EMBO molecular medicine 2024-08-05
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Down syndrome and postoperative hemodynamics in patients undergoing surgery for congenital cardiac communications
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Scientific reports 2024-07-18
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Development of a targeted IL-12 immunotherapy platform for B-cell lymphomas
Authors: Lee, D;Kim, D;Kim, D;Kim, N;Nam, YW;Lee, BC;Song, J;Chang, J;
International immunopharmacology 2024-07-12
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Hypoxia and inflammation induce synergistic transcriptome turnover in macrophages
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Cell reports 2024-07-04
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Paracrinal regulation of neutrophil functions by coronaviral infection in iPSC-derived alveolar type II epithelial cells
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Virus research 2024-07-01
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Dissecting the MUC5AC/ANXA2 signaling axis: implications for brain metastasis in lung adenocarcinoma
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Experimental & molecular medicine 2024-06-03
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Oncogenic KEAP1 mutations activate TRAF2-NF?B signaling to prevent apoptosis in lung cancer cells
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Redox biology 2024-02-01
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Targeting DHX9 triggers tumor-intrinsic interferon response and replication stress in Small Cell Lung Cancer
Authors: Murayama, T;Nakayama, J;Jiang, X;Miyata, K;Morris, AD;Cai, KQ;Prasad, RM;Ma, X;Efimov, A;Belani, N;Gerstein, ER;Tan, Y;Zhou, Y;Kim, W;Maruyama, R;Campbell, KS;Chen, L;Yang, Y;Balachandran, S;Canadas, I;
Cancer discovery 2024-01-08
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iPSC-derived PSEN2 (N141I) astrocytes and microglia exhibit a primed inflammatory phenotype
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Journal of neuroinflammation 2024-01-04
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The Effects of Preeclamptic Milieu on Cord Blood Derived Endothelial Colony-Forming Cells
Authors: Hall, E;Alderfer, L;Neu, E;Saha, S;Johandes, E;Haas, DM;Haneline, LS;Hanjaya-Putra, D;
bioRxiv : the preprint server for biology 2023-12-05
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Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation
Authors: Hong, J;Son, M;Sin, J;Kim, H;Chung, DK;
Nutrients 2023-11-09
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Mesenchymal stem cell-derived secretomes-enriched alginate/ extracellular matrix hydrogel patch accelerates skin wound healing
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Biomaterials research 2023-10-31
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Development, Characterization and Pharmacological Evaluation of Cannabidiol-Loaded Long Circulating Niosomes
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Pharmaceutics 2023-10-03
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Longitudinal changes in tear cytokines and antimicrobial proteins in trachomatous disease
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PLoS neglected tropical diseases 2023-10-01
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Regulatory role of human endometrial gland secretome on macrophage differentiation
Authors: Lin, L;Bai, K;Li, J;Chiu, PCN;Lee, CL;
Journal of reproductive immunology 2023-09-30
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New JAK3-INSL3 Fusion Transcript-An Oncogenic Event in Cutaneous T-Cell Lymphoma
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Cells 2023-09-29
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Vascular smooth muscle cells enhance immune/vascular interplay in a 3-cell model of vascular inflammation
Authors: Wiejak, J;Murphy, FA;Maffia, P;Yarwood, SJ;
Scientific reports 2023-09-23
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Head and Neck Squamous Cell Carcinoma Biopsies Maintained Ex Vivo on a Perfusion Device Show Gene Changes with Time and Clinically Relevant Doses of Irradiation
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Cancers 2023-09-15
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FBXO21 mediated degradation of p85? regulates proliferation and survival of acute myeloid leukemia
Authors: Dobish, KK;Wittorf, KJ;Swenson, SA;Bean, DC;Gavile, CM;Woods, NT;Ghosal, G;Hyde, RK;Buckley, SM;
Leukemia 2023-09-09
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Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53
Authors: Lee, JD;Menasche, BL;Mavrikaki, M;Uyemura, MM;Hong, SM;Kozlova, N;Wei, J;Alfajaro, MM;Filler, RB;Müller, A;Saxena, T;Posey, RR;Cheung, P;Muranen, T;Heng, YJ;Paulo, JA;Wilen, CB;Slack, FJ;
bioRxiv : the preprint server for biology 2023-09-01
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Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation
Authors: Shen, CJ;Chan, RH;Lin, BW;Li, NC;Huang, YH;Chang, WC;Chen, BK;
Theranostics 2023-08-21
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SEMA4A promotes prostate cancer invasion: involvement of tumor microenvironment
Authors: Liu, X;Tan, W;Wang, W;Feng, T;Wang, C;Wang, L;Zhou, W;
Journal of Cancer 2023-08-21
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Macrophage Profiling in Head and Neck Cancer to Improve Patient Prognosis and Assessment of Cancer Cell-Macrophage Interactions Using Three-Dimensional Coculture Models
Authors: Mhaidly, N;Journe, F;Najem, A;Stock, L;Trelcat, A;Dequanter, D;Saussez, S;Descamps, G;
International journal of molecular sciences 2023-08-15
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Mechanism and therapeutic implications of pomalidomide-induced immune surface marker upregulation in EBV-positive lymphomas
Authors: Jaeger, HK;Davis, DA;Nair, A;Shrestha, P;Stream, A;Yaparla, A;Yarchoan, R;
Scientific reports 2023-07-18
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Proteomics and phosphoproteomics profiling in glutamatergic neurons and microglia in an iPSC model of Jansen de Vries Syndrome
Authors: Aguilan, JT;Pedrosa, E;Dolstra, H;Baykara, RN;Barnes, J;Zhang, J;Sidoli, S;Lachman, HM;
bioRxiv : the preprint server for biology 2023-07-08
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The role of pneumococcal extracellular vesicles on the pathophysiology of the kidney disease hemolytic uremic syndrome
Authors: Battista, M;Hoffmann, B;Bachelot, Y;Zimmermann, L;Teuber, L;Jost, A;Linde, S;Westermann, M;Müller, MM;Slevogt, H;Hammerschmidt, S;Figge, MT;Vilhena, C;Zipfel, PF;
mSphere 2023-06-26
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Tanshinone IIA and Cryptotanshinone Counteract Inflammation by Regulating Gene and miRNA Expression in Human SGBS Adipocytes
Authors: Carpi, S;Quarta, S;Doccini, S;Saviano, A;Marigliano, N;Polini, B;Massaro, M;Carluccio, MA;Calabriso, N;Wabitsch, M;Santorelli, FM;Cecchini, M;Maione, F;Nieri, P;Scoditti, E;
Biomolecules 2023-06-23
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Matrix Metalloproteinase 9 Induced in Esophageal Squamous Cell Carcinoma Cells via Close Contact with Tumor-Associated Macrophages Contributes to Cancer Progression and Poor Prognosis
Authors: Tsukamoto, S;Koma, YI;Kitamura, Y;Tanigawa, K;Azumi, Y;Miyako, S;Urakami, S;Hosono, M;Kodama, T;Nishio, M;Shigeoka, M;Yokozaki, H;
Cancers 2023-05-30
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Regulation of Cancer Stem Cells and Epithelial-Mesenchymal Transition by CTNNAL1 in Lung Cancer and Glioblastoma
Authors: Kahm, YJ;Jung, U;Kim, RK;
Biomedicines 2023-05-17
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Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1
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PloS one 2023-05-16
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Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
Authors: Yndart Arias, A;Kolishetti, N;Vashist, A;Madepalli, L;Llaguno, L;Nair, M;
Scientific reports 2023-05-05
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Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling
Authors: Bian, F;Lan, YW;Zhao, S;Deng, Z;Shukla, S;Acharya, A;Donovan, J;Le, T;Milewski, D;Bacchetta, M;Hozain, AE;Tipograf, Y;Chen, YW;Xu, Y;Shi, D;Kalinichenko, VV;Kalin, TV;
Nature communications 2023-05-04
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Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer
Authors: S Mukhopadhy, J Encarnació, EY Lin, ASW Sohn, H Zhang, JD Mancias, AC Kimmelman
Science Advances, 2023-04-19;9(16):eadf9284. 2023-04-19
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Constitutive secretion of pro-IL-18 allows keratinocytes to initiate inflammation during bacterial infection
Authors: AF Johnson, JS Sands, KM Trivedi, R Russell, DL LaRock, CN LaRock
PloS Pathogens, 2023-04-17;19(4):e1011321. 2023-04-17
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Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response
Authors: CY Lin, KY Huang, SH Kao, MS Lin, CC Lin, SC Yang, WC Chung, YH Chang, RJ Chein, PC Yang
Science Advances, 2023-04-07;9(14):eade9944. 2023-04-07
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Cancer-associated mesothelial cell-derived ANGPTL4 and STC1 promote the early steps of ovarian cancer metastasis
Authors: P Bajwa, K Kordylewic, A Bilecz, RR Lastra, K Wroblewski, Y Rinkevich, E Lengyel, HA Kenny
JCI Insight, 2023-03-22;0(0):. 2023-03-22
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The early neutrophil-committed progenitors aberrantly differentiate into immunoregulatory monocytes during emergency myelopoiesis
Authors: N Ikeda, H Kubota, R Suzuki, M Morita, A Yoshimura, Y Osada, K Kishida, D Kitamura, A Iwata, S Yotsumoto, D Kurotaki, K Nishimura, A Nishiyama, T Tamura, T Kamatani, T Tsunoda, M Murakawa, Y Asahina, Y Hayashi, H Harada, Y Harada, A Yokota, H Hirai, T Seki, M Kuwahara, M Yamashita, S Shichino, M Tanaka, K Asano
Cell Reports, 2023-02-28;42(3):112165. 2023-02-28
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Cyanobacterial Harmful Bloom Lipopolysaccharides Induce Pro-Inflammatory Effects in Immune and Intestinal Epithelial Cells In Vitro
Authors: V Sko?ková, O Vaší?ek, E Sychrová, I Sovadinová, P Babica, L Šindlerová
Toxins, 2023-02-21;15(3):. 2023-02-21
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Vitamin D (1alpha,25(OH)2D3) supplementation minimized multinucleated giant cells formation and inflammatory response during Burkholderia pseudomallei infection in human lung epithelial cells
Authors: P Mattrasong, S Wongkaewkh, S Taweechais, S Chareonsud, T Techawiwat, T Ngamsiri, S Kanthawong
PLoS ONE, 2023-02-09;18(2):e0280944. 2023-02-09
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The D405N Mutation in the Spike Protein of SARS-CoV-2 Omicron BA.5 Inhibits Spike/Integrins Interaction and Viral Infection of Human Lung Microvascular Endothelial Cells
Authors: A Bugatti, F Filippini, S Messali, M Giovanetti, C Ravelli, A Zani, M Ciccozzi, A Caruso, F Caccuri
Viruses, 2023-01-24;15(2):. 2023-01-24
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Radiofrequency Ablation Remodels the Tumor Microenvironment and Promotes Neutrophil-Mediated Abscopal Immunomodulation in Pancreatic Cancer
Authors: EY Faraoni, BJ O'Brien, LN Strickland, BK Osborn, V Mota, J Chaney, CL Atkins, P Cen, J Rowe, J Cardenas, KL Poulsen, CJ Wray, NC Thosani, JM Bailey-Lun
Cancer Immunology Research, 2023-01-03;11(1):4-12. 2023-01-03
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EGFR-Tyrosine Kinase Inhibitors Induced Activation of the Autocrine CXCL10/CXCR3 Pathway through Crosstalk between the Tumor and the Microenvironment in EGFR-Mutant Lung Cancer
Authors: SH Hong, N Kang, O Kim, SA Hong, J Park, J Kim, MA Lee, J Kang
Cancers, 2022-12-25;15(1):. 2022-12-25
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Inhibition of triple negative breast cancer-associated inflammation and progression by N- acylethanolamine acid amide hydrolase (NAAA)
Authors: O Benchama, MS Malamas, K Praveen, EC Ethier, MK Williams, A Makriyanni, HK Avraham
Scientific Reports, 2022-12-23;12(1):22255. 2022-12-23
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Clozapine Suppresses the Gene Expression and the Production of Cytokines and Up-Regulates Cyclooxygenase 2 mRNA in Human Astroglial Cells
Authors: Y Yuhas, S Ashkenazi, E Berent, A Weizman
Brain sciences, 2022-12-12;12(12):. 2022-12-12
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Non-small cell lung cancer-derived exosomes promote proliferation, phagocytosis, and secretion of microglia via exosomal microRNA in the metastatic microenvironment
Authors: P Chen, Y Li, R Liu, Y Xie, Y Jin, M Wang, Z Yu, W Wang, X Luo
Translational Oncology, 2022-12-01;27(0):101594. 2022-12-01
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Successful targeting of PD-1/PD-L1 with chimeric antigen receptor-natural killer cells and nivolumab in a humanized mouse cancer model
Authors: WN Liu, WY So, SL Harden, SY Fong, MXY Wong, WWS Tan, SY Tan, JKL Ong, R Rajarethin, M Liu, JY Cheng, L Suteja, JPS Yeong, NG Iyer, DW Lim, Q Chen
Science Advances, 2022-11-23;8(47):eadd1187. 2022-11-23
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Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility
Authors: C Villarroya, A Osorio, R Torres-Rui, D Gómez-Sánc, M Trakala, A Sánchez-Be, F Mercadillo, B Hurtado, B Pitarch, A Hernández-, A Gómez-Catu, D Rueda, J Perea, S Rodríguez-, M Malumbres, M Urioste
Science Advances, 2022-11-02;8(44):eabq5914. 2022-11-02
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Metabolic control of CD47 expression through LAT2-mediated amino acid uptake promotes tumor immune evasion
Authors: Z Wang, B Li, S Li, W Lin, Z Wang, S Wang, W Chen, W Shi, T Chen, H Zhou, E Yinwang, W Zhang, H Mou, X Chai, J Zhang, Z Lu, Z Ye
Nature Communications, 2022-10-23;13(1):6308. 2022-10-23
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Profound Effects of Dexamethasone on the Immunological State, Synthesis and Secretion Capacity of Human Testicular Peritubular Cells
Authors: YK Stepanov, JD Speidel, C Herrmann, N Schmid, R Behr, FM Köhn, JB Stöckl, U Pickl, M Trottmann, T Fröhlich, A Mayerhofer, H Welter
Cells, 2022-10-09;11(19):. 2022-10-09
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Immune activation is essential for the antitumor activity of EZH2 inhibition in urothelial carcinoma
Authors: A Piunti, K Meghani, Y Yu, AG Robertson, JR Podojil, KA McLaughlin, Z You, D Fantini, M Chiang, Y Luo, L Wang, N Heyen, J Qian, SD Miller, A Shilatifar, JJ Meeks
Science Advances, 2022-10-05;8(40):eabo8043. 2022-10-05
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Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation
Authors: F Caso, A Saviano, M Tasso, F Raucci, N Marigliano, S Passavanti, P Frallonard, R Ramonda, V Brancaleon, M Bucci, R Scarpa, L Costa, F Maione
Autoimmunity reviews, 2022-10-01;21(12):103207. 2022-10-01
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Cytokine profile of human limbal myofibroblasts: Key players in corneal antiviral response
Authors: A Domínguez-, Y Garfias
Cytokine, 2022-09-29;160(0):156047. 2022-09-29
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The lysosomal proteome of senescent cells contributes to the senescence secretome
Authors: M Rovira, R Sereda, D Pladevall-, V Ramponi, I Marin, M Maus, J Madrigal-M, A Díaz, F García, J Muñoz, AM Cuervo, M Serrano
Aging Cell, 2022-09-10;0(0):e13707. 2022-09-10
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Obstructive Sleep Apnea Syndrome In Vitro Model: Controlled Intermittent Hypoxia Stimulation of Human Stem Cells-Derived Cardiomyocytes
Authors: D Regev, S Etzion, H Haddad, J Gopas, A Goldbart
International Journal of Molecular Sciences, 2022-09-07;23(18):. 2022-09-07
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Exosomal B7-H4 from irradiated glioblastoma cells contributes to increase FoxP3 expression of differentiating Th1 cells and promotes tumor growth
Authors: Y Tian, C Liu, Z Li, M Ai, B Wang, K Du, W Liu, H Wang, P Yu, C Chen, J Lin, A Xu, R Li, W Zhang, Y Yuan
Redox Biology, 2022-08-27;56(0):102454. 2022-08-27
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Detection of Leptomonas seymouri narna-like virus in serum samples of visceral leishmaniasis patients and its possible role in disease pathogenesis
Authors: S Sukla, H Nath, M Kamran, SA Ejazi, N Ali, P Das, V Ravichandi, S Roy, S Biswas
Scientific Reports, 2022-08-24;12(1):14436. 2022-08-24
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A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states
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Relation of Macrophage Migration Inhibitory Factor to Pulmonary Hemodynamics and Vascular Structure and Carbamyl-Phosphate Synthetase I Genetic Variations in Pediatric Patients with Congenital Cardiac Shunts
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Oncogene, 2019-01-28;0(0):. 2019-01-28
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Diabetol Metab Syndr, 2019-01-25;11(0):9. 2019-01-25
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Cell Death Dis, 2019-01-25;10(2):68. 2019-01-25
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Suppression of chemotherapy-induced cytokine/lipid mediator surge and ovarian cancer by a dual COX-2/sEH inhibitor
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Proc. Natl. Acad. Sci. U.S.A., 2019-01-15;116(5):1698-1703. 2019-01-15
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Cancer Res., 2018-12-18;0(0):. 2018-12-18
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JCI Insight, 2018-12-06;3(23):. 2018-12-06
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Tobacco Carcinogen-Induced Production of GM-CSF Activates CREB to Promote Pancreatic Cancer
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Cancer Res., 2018-09-19;0(0):. 2018-09-19
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Modulating bone marrow hematopoietic lineage potential to prevent bone metastasis in breast cancer
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Cancer Res., 2018-07-31;0(0):. 2018-07-31
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Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
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Psychoneuroendocrinology, 2018-07-13;9(1):2705. 2018-07-13
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A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer's disease
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Nat. Neurosci., 2018-06-27;21(7):941-951. 2018-06-27
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Differential Sensitivity of Human Hepatocellular Carcinoma Xenografts to an IGF-II Neutralizing Antibody May Involve Activated STAT3
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Transl Oncol, 2018-06-22;11(4):971-978. 2018-06-22
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Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
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Sci Rep, 2018-05-14;8(1):7506. 2018-05-14
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Safety, pharmacokinetics, and biomarkers of F-652, a recombinant human interleukin-22 dimer, in healthy subjects
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Cell. Mol. Immunol., 2018-04-18;0(0):. 2018-04-18
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The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells
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Sci Rep, 2018-04-03;8(1):5426. 2018-04-03
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Multi-omics analysis reveals neoantigen-independent immune cell infiltration in copy-number driven cancers
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Nat Commun, 2018-04-03;9(1):1317. 2018-04-03
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Endothelial Protein C-Targeting Liposomes Show Enhanced Uptake and Improved Therapeutic Efficacy in Human Retinal Endothelial Cells
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Invest. Ophthalmol. Vis. Sci., 2018-04-01;59(5):2119-2132. 2018-04-01
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Overexpression of Rab11-FIP2 in colorectal cancer cells promotes tumor migration and angiogenesis through increasing secretion of PAI-1
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Cancer Cell Int., 2018-03-09;18(0):35. 2018-03-09
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Effects of Graphene Oxide Nanoparticles on the Immune System Biomarkers Produced by RAW 264.7 and Human Whole Blood Cell Cultures
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Nanomaterials (Basel), 2018-02-24;8(2):. 2018-02-24
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Neuropharmacology, 2018-01-31;133(0):121-128. 2018-01-31
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Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke
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Sci Rep, 2018-01-15;8(1):743. 2018-01-15
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HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome
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Oncogene, 2018-01-09;0(0):. 2018-01-09
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Gp96 Peptide Antagonist gp96-II Confers Therapeutic Effects in Murine Intestinal Inflammation
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Front Immunol, 2017-12-11;8(0):1531. 2017-12-11
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Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections
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Stem Cells Int, 2017-10-31;2017(0):5932706. 2017-10-31
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Stem Cell Res Ther, 2017-09-29;8(1):212. 2017-09-29
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Lipoprotein(a) Induces Human Aortic Valve Interstitial Cell Calcification
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Stem Cell Res Ther, 2017-07-27;8(1):177. 2017-07-27
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Cancer-Associated Fibroblasts Share Characteristics and Pro-tumorigenic Activity with Mesenchymal Stromal Cells
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Cancer Res., 2017-07-07;0(0):. 2017-07-07
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miRNA-1246 induces pro-inflammatory responses in mesenchymal stem/stromal cells by regulating PKA and PP2A
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Oncotarget, 2017-07-04;8(27):43897-43914. 2017-07-04
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The Effect of a p38 Mitogen-Activated Protein Kinase Inhibitor on Cellular Senescence of Cultivated Human Corneal Endothelial Cells
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Invest. Ophthalmol. Vis. Sci., 2017-07-01;58(9):3325-3334. 2017-07-01
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Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System
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Downregulation of UHRF1 increases tumor malignancy by activating the CXCR4/AKT-JNK/IL-6/Snail signaling axis in hepatocellular carcinoma cells
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Sci Rep, 2017-06-05;7(1):2798. 2017-06-05
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Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis
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Stem Cells, 2017-06-05;0(0):. 2017-06-05
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NOX2-dependent ATM kinase activation dictates pro-inflammatory macrophage phenotype and improves effectiveness to radiation therapy
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Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling
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PLoS ONE, 2017-04-13;12(4):e0175159. 2017-04-13
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Surface-bound galectin-4 regulates gene transcription and secretion of chemokines in human colorectal cancer cell lines
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Tumour Biol, 2017-03-01;39(3):1010428317691. 2017-03-01
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Orai and TRPC channel characterization in Fc?RI-mediated calcium signaling and mediator secretion in human mast cells
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Physiol Rep, 2017-03-01;5(5):. 2017-03-01
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Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
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Cancer Res, 2017-03-01;0(0):. 2017-03-01
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Serum cytokine/chemokine profiles in patients with dengue fever (DF) and dengue hemorrhagic fever (FHD) by using protein array
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J. Clin. Virol, 2017-02-17;89(0):39-45. 2017-02-17
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The TRAIL-Induced Cancer Secretome Promotes a Tumor-Supportive Immune Microenvironment via CCR2
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Mol. Cell, 2017-02-16;65(4):730-742.e5. 2017-02-16
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A Proinflammatory Secretome Mediates the Impaired Immunopotency of Human Mesenchymal Stromal Cells in Elderly Patients with Atherosclerosis
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Stem Cells Transl Med, 2017-02-14;6(4):1132-1140. 2017-02-14
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Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment
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Int J Mol Sci, 2017-02-14;18(2):. 2017-02-14
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Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells
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Oncotarget, 2017-01-03;8(1):1438-1448. 2017-01-03
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Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
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Autophagy, 2017-01-01;0(0):0. 2017-01-01
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Multimodal Regulation of NET Formation in Pregnancy: Progesterone Antagonizes the Pro-NETotic Effect of Estrogen and G-CSF
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Front Immunol, 2016-12-05;7(0):565. 2016-12-05
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Intracellular STING inactivation sensitizes breast cancer cells to genotoxic agents
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Oncotarget, 2016-11-22;7(47):77205-77224. 2016-11-22
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MicroRNAs regulate cytokine responses in gingival epithelial cells
Infect Immun, 2016-11-18;0(0):. 2016-11-18
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Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity
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J. Virol., 2016-09-29;90(20):9263-84. 2016-09-29
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Human and mouse monocytes display distinct signalling and cytokine profiles upon stimulation with FFAR2/FFAR3 short-chain fatty acid receptor agonists
Sci Rep, 2016-09-26;6(0):34145. 2016-09-26
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Neurotensin stimulates sortilin and mTOR in human microglia inhibitable by methoxyluteolin, a potential therapeutic target for autism
Proc Natl Acad Sci USA, 2016-09-23;0(0):. 2016-09-23
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MEK and TAK1 regulate apoptosis in colon cancer cells with KRAS-dependent activation of proinflammatory signaling
Mol Cancer Res, 2016-09-21;0(0):. 2016-09-21
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The oncometabolite R-2-hydroxyglutarate activates NF-?B-dependent tumor-promoting stromal niche for acute myeloid leukemia cells
Sci Rep, 2016-08-31;6(0):32428. 2016-08-31
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Serum Cytokines in Young Pediatric Patients with Congenital Cardiac Shunts and Altered Pulmonary Hemodynamics
Mediators Inflamm, 2016-08-30;2016(0):7672048. 2016-08-30
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The MNK-1/eIF4E pathway as a new therapeutic pathway to target inflammation and remodelling in asthma
Cell Signal, 2016-07-11;28(10):1555-1562. 2016-07-11
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MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC.
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Sci Rep, 2016-07-06;6(0):29160. 2016-07-06
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IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
Authors: Matthew N Svalina
Sci Rep, 2016-06-03;6(0):27012. 2016-06-03
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NF-?B-HOTAIR axis links DNA damage response, chemoresistance and cellular senescence in ovarian cancer
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Oncogene, 2016-04-04;0(0):. 2016-04-04
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Soluble alpha-enolase activates monocytes by CD14-dependent TLR4 signalling pathway and exhibits a dual function
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Sci Rep, 2016-03-30;6(0):23796. 2016-03-30
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Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells.
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Activation of IFN/STAT1 signalling predicts response to chemotherapy in oestrogen receptor-negative breast cancer
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Fibrocytes Differ from Macrophages but Can Be Infected with HIV-1.
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In vitro Study of a Novel Stent Coating Using Modified CD39 Messenger RNA to Potentially Reduce Stent Angioplasty-Associated Complications.
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PLoS ONE, 2015-09-18;10(9):e0138375. 2015-09-18
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Blood, 2015-06-22;126(9):1106-17. 2015-06-22
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High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts.
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PLoS ONE, 2015-04-28;10(4):e0124283. 2015-04-28
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Diurnal rhythms of serum and plasma cytokine profiles in healthy elderly individuals assessed using membrane based multiplexed immunoassay.
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J Transl Med, 2015-04-24;13(0):129. 2015-04-24
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XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer.
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Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling.
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Macrophage migration inhibitory factor-CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors.
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Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells.
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Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application.
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The role of TLR8 signaling in acute myeloid leukemia differentiation.
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M-CSF inhibits anti-HIV-1 activity of IL-32, but they enhance M2-like phenotypes of macrophages.
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A cannabigerol derivative suppresses immune responses and protects mice from experimental autoimmune encephalomyelitis.
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Identification of novel non-coding RNA-based negative feedback regulating the expression of the oncogenic transcription factor GLI1.
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IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction.
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Human adipose-derived stromal/stem cells induce functional CD4+CD25+FoxP3+CD127- regulatory T cells under low oxygen culture conditions.
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Cytokine/chemokine secretion and proteomic identification of upregulated annexin A1 from peripheral blood mononuclear cells cocultured with the liver fluke Opisthorchis viverrini.
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Moraxella catarrhalis adhesin UspA1-derived recombinant fragment rD-7 induces monocyte differentiation to CD14+CD206+ phenotype.
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The biological effect of large single doses: a possible role for non-targeted effects in cell inactivation.
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The RNA chaperone Hfq is involved in stress tolerance and virulence in uropathogenic Proteus mirabilis.
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Slit2-Robo4 pathway modulates lipopolysaccharide-induced endothelial inflammation and its expression is dysregulated during endotoxemia.
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Cold atmospheric plasma (CAP) changes gene expression of key molecules of the wound healing machinery and improves wound healing in vitro and in vivo.
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miR-200b and cancer/testis antigen CAGE form a feedback loop to regulate the invasion and tumorigenic and angiogenic responses of a cancer cell line to microtubule-targeting drugs.
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Development, characterization, and reversal of acquired resistance to the MEK1 inhibitor selumetinib (AZD6244) in an in vivo model of childhood astrocytoma.
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Gremlin-1 is an inhibitor of macrophage migration inhibitory factor and attenuates atherosclerotic plaque growth in ApoE-/- Mice.
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Anticancer compound ABT-263 accelerates apoptosis in virus-infected cells and imbalances cytokine production and lowers survival rates of infected mice.
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Comparison of endometrial regenerative cells and bone marrow stromal cells.
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LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.
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Age-related changes in expression and function of Toll-like receptors in human skin.
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Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
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Obatoclax, saliphenylhalamide, and gemcitabine inhibit influenza a virus infection.
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Tumor Microenvironment Macrophage Inhibitory Factor Directs the Accumulation of Interleukin-17-producing Tumor-infiltrating Lymphocytes and Predicts Favorable Survival in Nasopharyngeal Carcinoma Patients.
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Differential interaction of the two related fungal species Candida albicans and Candida dubliniensis with human neutrophils.
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Primary tumor hypoxia recruits CD11b+/Ly6Cmed/Ly6G+ immune suppressor cells and compromises NK cell cytotoxicity in the premetastatic niche.
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A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
Acta Pharmacol. Sin., 2012-06-11;33(9):1204-16. 2012-06-11
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Characterization of a Mutated IgA2 Antibody of the m(1) Allotype against the Epidermal Growth Factor Receptor for the Recruitment of Monocytes and Macrophages.
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Anti-inflammatory effects of methoxyphenolic compounds on human airway cells.
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Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts.
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PLoS ONE, 2012-03-13;7(3):e33474. 2012-03-13
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HIV-1 proteins preferentially activate anti-inflammatory M2-type macrophages.
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Inhibition of IL-32 activation by alpha-1 antitrypsin suppresses alloreactivity and increases survival in an allogeneic murine marrow transplantation model.
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Blood, 2011-09-06;118(18):5031-9. 2011-09-06
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Fine-tuning nucleophosmin in macrophage differentiation and activation.
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Blood, 2011-08-29;118(17):4694-704. 2011-08-29
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Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
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Diabetologia, 2011-06-10;54(9):2358-67. 2011-06-10
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The immunomodulatory role of syncytiotrophoblast microvesicles.
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Proc. Natl. Acad. Sci. U.S.A., 2011-05-02;108(20):8402-7. 2011-05-02
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Maternal high-fat diet disturbs uteroplacental hemodynamics and increases the frequency of stillbirth in a nonhuman primate model of excess nutrition.
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Endocrinology, 2011-03-29;152(6):2456-64. 2011-03-29
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Targeting Galectin-1 in Carcinoma-Associated Fibroblasts Inhibits Oral Squamous Cell Carcinoma Metastasis by Downregulating MCP-1/CCL2 Expression.
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Clin. Cancer Res., 2011-03-08;17(6):1306-16. 2011-03-08
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Syndecan-1 knock-down in decidualized human endometrial stromal cells leads to significant changes in cytokine and angiogenic factor expression patterns.
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Reprod. Biol. Endocrinol., 2010-11-02;8(0):133. 2010-11-02
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IL-37 is a fundamental inhibitor of innate immunity.
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Nat. Immunol., 2010-10-10;11(11):1014-22. 2010-10-10
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Pancreatic adenocarcinoma upregulated factor promotes metastasis by regulating TLR/CXCR4 activation.
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Oncogene, 2010-08-30;30(2):201-11. 2010-08-30
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Androgen-regulated expression of arginase 1, arginase 2 and interleukin-8 in human prostate cancer.
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PLoS ONE, 2010-08-11;5(8):e12107. 2010-08-11
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A role for the human nucleotide-binding domain, leucine-rich repeat-containing family member NLRC5 in antiviral responses.
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Interleukin-27 induces a STAT1/3- and NF-kappaB-dependent proinflammatory cytokine profile in human monocytes.
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PI3K/p110{delta} is a novel therapeutic target in multiple myeloma.
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Blood, 2010-05-26;116(9):1460-8. 2010-05-26
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Chlamydia pneumoniae infection in polarized epithelial cell lines.
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Infect. Immun., 2010-03-29;78(6):2714-22. 2010-03-29
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Glucose-induced expression of MIP-1 genes requires O-GlcNAc transferase in monocytes.
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Biochem. Biophys. Res. Commun., 2010-03-02;394(4):865-70. 2010-03-02
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Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.
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PLoS ONE, 2010-02-24;5(2):e9357. 2010-02-24
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Changes in melanocortin expression and inflammatory pathways in fetal offspring of nonhuman primates fed a high-fat diet.
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Endocrinology, 2010-02-22;151(4):1622-32. 2010-02-22
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Human CD4- 8- T cells are a distinctive immunoregulatory subset.
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FASEB J, 2010-02-12;24(7):2558-66. 2010-02-12
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Alpha-defensins secreted by dysplastic granulocytes inhibit the differentiation of monocytes in chronic myelomonocytic leukemia.
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Blood, 2009-10-28;115(1):78-88. 2009-10-28
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Macrophage-derived IL-1beta stimulates Wnt signaling and growth of colon cancer cells: a crosstalk interrupted by vitamin D3.
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Oncogene, 2009-08-24;28(44):3892-902. 2009-08-24
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Glioblastoma and endothelial cells cross-talk, mediated by SDF-1, enhances tumour invasion and endothelial proliferation by increasing expression of cathepsins B, S, and MMP-9.
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Cancer Lett., 2009-08-22;289(1):53-61. 2009-08-22
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Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.
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ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency.
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Blood, 2009-07-24;114(15):3216-26. 2009-07-24
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Identification of macrophage migration inhibitory factor and human neutrophil peptides 1-3 as potential biomarkers for gastric cancer.
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Br. J. Cancer, 2009-06-23;101(2):295-302. 2009-06-23
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Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
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Prostate cancer promotes CD11b positive cells to differentiate into osteoclasts.
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In vitro neutralization of tumor necrosis factor-alpha during Chlamydia pneumoniae infection impairs dendritic cells maturation/function and increases chlamydial progeny.
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Soluble CD40 ligand as a biomarker for storage-related preanalytic variations of human serum.
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Co-cultures of multiple cell types mimic pulmonary cell communication in response to urban PM10.
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Endogenous IL-32 controls cytokine and HIV-1 production.
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Agonists of proteinase-activated receptor-2 enhance IFN-gamma-inducible effects on human monocytes: role in influenza A infection.
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Dysregulation of IL-32 in myelodysplastic syndrome and chronic myelomonocytic leukemia modulates apoptosis and impairs NK function.
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IL-1beta-stimulated activation of ERK1/2 and p38alpha MAPK mediates the transcriptional up-regulation of IL-6, IL-8 and GRO-alpha in HeLa cells.
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Dasatinib, a small-molecule protein tyrosine kinase inhibitor, inhibits T-cell activation and proliferation.
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Annexin A2 is a soluble mediator of macrophage activation.
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A paracrine role for chemoattractant receptor-homologous molecule expressed on T helper type 2 cells (CRTH2) in mediating chemotactic activation of CRTH2+ CD4+ T helper type 2 lymphocytes.
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Immunology, 2007-04-16;121(4):577-84. 2007-04-16
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Induction of host chemotactic response by Encephalitozoon spp.
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FAQs
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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Tested condition media of MSCs from the umbilical cord co-cultured with NK cells. The goal of this experiments was to detect chages in inflammatory factors in the system when MSCs were cultured with NK cells. The lack of detected secreted proteins is promising because this suggests that MSCs do not up-regulate large inflammatory proteins following immune cell activation.
Left image : MSC cultured in RPMI media (Control)
Right image: MSC co-cultured with NK cells in same RPMI media