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Human TREM-1 Quantikine ELISA Kit

Catalog #: DTRM10B
14 Citations Datasheet / COA / SDS

Discontinued Product

DTRM10B has been discontinued.
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human TREM-1 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
Sensitivity
30.6 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human TREM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human TREM-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TREM-1 in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TREM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring TREM-1.

Recovery

The recovery of TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 109 97-119
EDTA Plasma (n=4) 98 86-108
Heparin Plasma (n=4) 99 85-109
Serum (n=4) 98 87-110

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of TREM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TREM-1 ELISA Linearity

Scientific Data

N/A Human TREM-1 ELISA Standard Curve View Larger

Human TREM-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: TREM-1

The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.

Long Name:
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs:
54210 (Human); 58217 (Mouse)
Alternate Names:
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

Citations for Human TREM-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients
    Authors: V Montiel, I Lobysheva, L Gérard, M Vermeersch, D Perez-Morg, T Castelein, JB Mesland, P Hantson, C Collienne, D Gruson, MA van Dievoe, A Persu, C Beauloye, M Dechamps, L Belkhir, A Robert, M Derive, PF Laterre, AHJ Danser, X Wittebole, JL Balligand
    EBioMedicine, 2022-02-23;77(0):103893.
    Species: Human
    Sample Types: Plasma
  2. Increased sTREM-1 plasma concentrations are associated with poor clinical outcomes in patients with COVID-19
    Authors: AH de Nooijer, I Grondman, S Lambden, EJ Kooistra, NA Janssen, M Kox, P Pickkers, LA Joosten, F van de Vee, M Derive, S Gibot, MG Netea
    Bioscience Reports, 2021-07-30;0(0):.
    Species: Human
    Sample Types: Plasma
  3. Diagnostic value of serum soluble triggering expressed receptor on myeloid cells 1 (sTREM-1) in suspected sepsis: a meta-analysis
    Authors: W Chang, F Peng, SS Meng, JY Xu, Y Yang
    BMC Immunol., 2020-01-13;21(1):2.
    Species: Human
    Sample Types: Serum
  4. Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation
    Authors: Y He, Q Yang, X Wang, A Jia, W Xie, J Zhou
    Mediators Inflamm., 2019-12-06;2019(0):5647074.
    Species: Human
    Sample Types: Plasma
  5. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells
    Authors: J Hyun, RS McMahon, AL Lang, JS Edwards, AD Badilla, ME Greene, GW Stone, S Pallikkuth, M Stevenson, DM Dykxhoorn, S Kottilil, S Pahwa, E Thomas
    PLoS Pathog., 2019-07-01;15(7):e1007883.
    Species: Human
    Sample Types: Cell Lysates
  6. Role of TREM-1 in pulmonary tuberculosis patients- analysis of serum soluble TREM-1 levels
    Authors: JY Feng, WJ Su, SW Pan, YC Yeh, YY Lin, NJ Chen
    Sci Rep, 2018-05-29;8(1):8223.
    Species: Human
    Sample Types: Serum
  7. Pleiotropy of ABO gene: correlation of rs644234 with E-selectin and lipid levels
    Authors: AA Arguinano, NC Ndiaye, C Masson, S Visvikis-S
    Clin. Chem. Lab. Med., 2018-04-25;0(0):.
    Species: Human
    Sample Types: Serum
  8. TREM-1 SNP rs2234246 regulates TREM-1 protein and mRNA levels and is associated with plasma levels of L-selectin
    Authors: AA Aldasoro A, S Dadé, M Stathopoul, M Derive, N Coumba Ndi, T Xie, C Masson, S Gibot, S Visvikis-S
    PLoS ONE, 2017-08-03;12(8):e0182226.
    Species: Human
    Sample Types: Serum
  9. Urine TREM-1 as a marker of urinary tract infection in children
    Authors: E Sierra-Dia, A Bravo Cuél, PC Ortiz Laza, M García Gut, HF Georgina, R Anaya Prad
    J. Int. Med. Res., 2017-04-02;45(2):631-638.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Decoy receptor 3 levels in peripheral blood predict outcomes of acute respiratory distress syndrome.
    Authors: Chen CY, Yang KY, Chen MY, Chen HY, Lin MT, Lee YC, Perng RP, Hsieh SL, Yang PC, Chou TY
    Am. J. Respir. Crit. Care Med., 2009-07-30;180(8):751-60.
    Species: Human
    Sample Types: Plasma
  11. Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis.
    Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H
    Arthritis Rheum., 2009-06-01;60(6):1615-23.
    Species: Human
    Sample Types: Serum
  12. Correlation of serum-soluble triggering receptor expressed on myeloid cells-1 with clinical disease activity in inflammatory bowel disease.
    Authors: Park JJ, Cheon JH, Kim BY, Kim DH, Kim ES, Kim TI, Lee KR, Kim WH
    Dig. Dis. Sci., 2008-10-31;54(7):1525-31.
    Species: Human
    Sample Types: Serum
  13. Increased levels of soluble triggering receptor expressed on myeloid cells-1 in patients with acute pancreatitis.
    Authors: Yasuda T, Takeyama Y, Ueda T, Shinzeki M, Sawa H, Takahiro N, Kamei K, Ku Y, Kuroda Y, Ohyanagi H
    Crit. Care Med., 2008-07-01;36(7):2048-53.
    Species: Human
    Sample Types: Serum
  14. TREM-1 expression in tumor-associated macrophages and clinical outcome in lung cancer.
    Authors: Ho CC, Liao WY, Wang CY, Lu YH, Huang HY, Chen HY, Chan WK, Chen HW, Yang PC
    Am. J. Respir. Crit. Care Med., 2007-12-20;177(7):763-70.
    Species: Human
    Sample Types: Cell Culture Supernates

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