Home / CXCL1/GRO alpha/KC/CINC-1 / Human CXCL1/GRO alpha DuoSet ELISA

Human CXCL1/GRO alpha DuoSet ELISA

Catalog #: DY275
60 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY275
DY275-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human CXCL1 / GRO alpha / KC / CINC-1 ELISA Standard Curve
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Product Details
Procedure
Citations (60)
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Supplemental Products
Reviews (4)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CXCL1/GRO alpha DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CXCL1/GROalpha. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human CXCL1 / GRO alpha / KC / CINC-1 ELISA Standard Curve View Larger

Human CXCL1 / GRO alpha / KC / CINC-1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Entrez Gene IDs:
2919 (Human); 14825 (Mouse); 81503 (Rat)
Alternate Names:
CINC1; CINC-1; CXCL1; FSP; GRO alpha; GRO1; GROa; KC; MGSA; MGSA-a; MGSA-alpha; NAP-3; SCYB1

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human CXCL1/GRO alpha DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

60 Citations: Showing 1 - 10
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  1. Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing
    Authors: Reedy, JL;Jensen, KN;Crossen, AJ;Basham, KJ;Ward, RA;Reardon, CM;Brown Harding, H;Hepworth, OW;Simaku, P;Kwaku, GN;Tone, K;Willment, JA;Reid, DM;Stappers, MHT;Brown, GD;Rajagopal, J;Vyas, JM;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Dietary Polyphenols Decrease Chemokine Release by Human Primary Astrocytes Responding to Pro-Inflammatory Cytokines
    Authors: Grabarczyk, M;Ksiazek-Winiarek, D;Glabinski, A;Szpakowski, P;
    Pharmaceutics
    Species: Human
    Sample Types: Cell Culture Supernates
  3. c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities
    Authors: Liu, Y;Smith, MR;Wang, Y;D'Agostino, R;Ruiz, J;Lycan, T;Kucera, GL;Miller, LD;Li, W;Chan, MD;Farris, M;Su, J;Song, Q;Zhao, D;Chandrasekaran, A;Xing, F;
    Cancers
  4. Cannabidiol as a Promising Therapeutic Option in IC/BPS: In Vitro Evaluation of Its Protective Effects against Inflammation and Oxidative Stress
    Authors: T Kuret, ME Kreft, R Romih, P Verani?
    International Journal of Molecular Sciences, 2023-03-06;24(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. SCCA1/SERPINB3 promotes suppressive immune environment via STAT-dependent chemokine production, blunting the therapy-induced T cell responses
    Authors: L Chen, V Shi, S Wang, R Freeman, F Ruiz, K Jayachandr, J Zhang, P Cosper, L Sun, CJ Luke, C Spina, PW Grigsby, JK Schwarz, S Markovina
    bioRxiv : the preprint server for biology, 2023-02-03;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer
    Authors: J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi
    Cancer Research, 2022-11-15;0(0):OF1-OF14.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
    Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Journal of ovarian research, 2022-07-26;15(1):87.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli
    Authors: P Szpakowski, D Ksiazek-Wi, M Turniak-Ku, I Pacan, A Glabinski
    Biomedicines, 2022-07-22;10(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells
    Authors: E Filidou, L Kandilogia, G Tarapatzi, M Spathakis, P Steiropoul, D Mikroulis, K Arvanitidi, V Paspaliari, G Kolios
    International Journal of Molecular Sciences, 2022-04-20;23(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Microsatellite Status and IkappaBalpha Expression Levels Predict Sensitivity to Pharmaceutical Curcumin in Colorectal Cancer Cells
    Authors: L Lu, R Przybylla, Y Shang, M Dai, M Krohn, OH Krämer, CS Mullins, M Linnebache
    Cancers, 2022-02-17;14(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models
    Authors: J Queen, JC Domingue, JR White, C Stevens, B Udayasurya, TTD Nguyen, S Wu, H Ding, H Fan, M McMann, A Corona, TC Larman, SS Verbridge, F Housseau, DJ Slade, JL Drewes, CL Sears
    MBio, 2022-02-08;0(0):e0299121.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Differential expression profile of CXC-receptor-2 ligands as potential biomarkers in pancreatic ductal adenocarcinoma
    Authors: S Saxena, C Molczyk, A Purohit, E Ehrhorn, P Goel, DR Prajapati, P Atri, S Kaur, PM Grandgenet, MA Hollingswo, SK Batra, RK Singh
    American journal of cancer research, 2022-01-15;12(1):68-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Atypical response to bacterial co-infection and persistent neutrophilic broncho-alveolar inflammation distinguish critical COVID-19 from influenza
    Authors: S Cambier, M Metzemaeke, AC Carvalho, A Nooyens, C Jacobs, L Vanderbeke, B Malengier-, M Gouwy, E Heylen, P Meersseman, G Hermans, E Wauters, A Wilmer, C Consortium, D Schols, P Matthys, G Opdenakker, RE Marques, J Wauters, J Vandooren, P Proost
    JCI Insight, 2022-01-11;0(0):.
    Species: Human
    Sample Types: BAL Supernatant
  14. PAD4 controls chemoattractant production and neutrophil trafficking in malaria
    Authors: D Cela, SL Knackstedt, S Groves, CM Rice, JTW Kwon, B Mordmüller, B Amulic
    Journal of leukocyte biology, 2021-11-10;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. IL-6 promotes drug resistance through formation of polyploid giant cancer cells and stromal fibroblast reprogramming
    Authors: N Niu, J Yao, RC Bast, AK Sood, J Liu
    Oncogenesis, 2021-09-29;10(9):65.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade
    Authors: Z Yang, G Xu, B Wang, Y Liu, L Zhang, T Jing, M Tang, X Xu, K Jiao, L Xiang, Y Fu, D Tang, X Zhang, W Jin, G Zhuang, X Zhao, Y Liu
    Nature Communications, 2021-08-11;12(1):4852.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Caspase-8 mutations associated with head and neck cancer differentially retain functional properties related to TRAIL-induced apoptosis and cytokine induction
    Authors: Z Cui, H Dabas, BC Leonard, JV Shiah, JR Grandis, DE Johnson
    Cell Death & Disease, 2021-08-06;12(8):775.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis
    Authors: L Kandilogia, E Filidou, I Drygiannak, G Tarapatzi, S Didaskalou, M Koffa, K Arvanitidi, G Bamias, V Valatas, V Paspaliari, G Kolios
    Stem Cells International, 2021-07-27;2021(0):9929461.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Csnk1a1 inhibition modulates the inflammatory secretome and enhances response to radiotherapy in glioma
    Authors: G Liu, H Li, W Zhang, J Yu, X Zhang, R Wu, M Niu, X Liu, R Yu
    Journal of Cellular and Molecular Medicine, 2021-07-03;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells
    Authors: S Xia, J Wu, W Zhou, M Zhang, K Zhao, J Liu, D Tian, J Liao
    Cell Death & Disease, 2021-06-02;12(6):570.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration
    Authors: HK Skovdahl, S Gopalakris, TD Svendsen, AVB Granlund, I Bakke, ZG Ginbot, S Thorsvik, A Flatberg, B Sporsheim, J Ostrop, TE Mollnes, AK Sandvik, T Bruland
    Frontiers in Pharmacology, 2021-05-13;12(0):679741.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Gain-of-function variants in SYK cause immune dysregulation and systemic inflammation in humans and mice
    Authors: L Wang, D Aschenbren, Z Zeng, X Cao, D Mayr, M Mehta, M Capitani, N Warner, J Pan, L Wang, Q Li, T Zuo, S Cohen-Keda, J Lu, RC Ardy, DJ Mulder, D Dissanayak, K Peng, Z Huang, X Li, Y Wang, X Wang, S Li, S Bullers, AN Gammage, K Warnatz, AI Schiefer, G Krivan, V Goda, WHA Kahr, M Lemaire, Genomics E, CY Lu, I Siddiqui, MG Surette, D Kotlarz, KR Engelhardt, HR Griffin, R Rottapel, H Decaluwe, RM Laxer, M Proietti, S Hambleton, S Elcombe, CH Guo, B Grimbacher, I Dotan, SC Ng, SA Freeman, SB Snapper, C Klein, K Boztug, Y Huang, D Li, HH Uhlig, AM Muise
    Nature Genetics, 2021-03-29;53(4):500-510.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
    Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
    Biomolecules, 2021-02-21;11(2):.
    Species: Human
    Sample Types: Plasma
  24. Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist
    Authors: H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier
    Frontiers in Pharmacology, 2020-12-08;11(0):598702.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. New insights in gene expression alteration as effect of doxorubicin drug resistance in triple negative breast cancer cells
    Authors: CA Ciocan-Car, A Jurj, O Zanoaga, R Cojocneanu, LA Pop, A Moldovan, C Moldovan, AA Zimta, L Raduly, C Pop-Bica, M Buse, L Budisan, P Virag, A Irimie, SMG Diaz, I Berindan-N, C Braicu
    J Exp Clin Cancer Res, 2020-11-13;39(1):241.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1
    Authors: J Sellau, M Groneberg, H Fehling, T Thye, S Hoenow, C Marggraff, M Weskamm, C Hansen, S Stanelle-B, S Kuehl, J Noll, V Wolf, NG Metwally, SH Hagen, C Dorn, J Wernecke, H Ittrich, E Tannich, T Jacobs, I Bruchhaus, M Altfeld, H Lotter
    Nat Commun, 2020-07-10;11(1):3459.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Constitutive Interferon Attenuates RIPK1/3-Mediated Cytokine Translation
    Authors: HI Muendlein, J Sarhan, BC Liu, WM Connolly, SA Schworer, I Smirnova, AY Tang, V Ilyukha, J Pietruska, S Tahmasebi, N Sonenberg, A Degterev, A Poltorak
    Cell Rep, 2020-01-21;30(3):699-713.e4.
    Species: Human
    Sample Types: Cell Culture Media
  28. Bleomycin hydrolase regulates the release of chemokines important for inflammation and wound healing by keratinocytes
    Authors: R Riise, L Odqvist, J Mattsson, S Monkley, SM Abdillahi, C Tyrchan, D Muthas, LF Yrlid
    Sci Rep, 2019-12-31;9(1):20407.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Reduced expression of miR-146a in human bronchial epithelial cells alters neutrophil migration
    Authors: A Kivihall, A Aab, J Soja, K S?adek, M Sanak, A Altraja, B Jakiela, G Bochenek, A Rebane
    Clin Transl Allergy, 2019-11-27;9(0):62.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Regulation of CXCL1 chemokine and CSF3 cytokine levels in myometrial cells by the MAFF transcription factor
    Authors: J Saliba, B Coutaud, V Solovieva, F Lu, V Blank
    J. Cell. Mol. Med., 2019-01-22;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Neutrophil recruitment to inflamed joints can occur without cellular priming
    Authors: L Björkman, K Christenso, L Davidsson, J Mårtensson, F Amirbeagi, A Welin, H Forsman, A Karlsson, C Dahlgren, J Bylund
    J. Leukoc. Biol., 2018-12-20;0(0):.
    Species: Human
    Sample Types: Serum
  32. Rhinovirus and Bacteria Synergistically Induce IL-17C Release from Human Airway Epithelial Cells To Promote Neutrophil Recruitment
    Authors: KC Jamieson, SL Traves, C Kooi, S Wiehler, CJ Dumonceaux, BA Maciejewsk, JW Arnason, AN Michi, R Leigh, D Proud
    J. Immunol., 2018-11-30;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. The small molecule rhodomyrtone suppresses TNF-? and IL-17A-induced keratinocyte inflammatory responses: A potential new therapeutic for psoriasis
    Authors: J Chorachoo, S Lambert, T Furnholm, L Roberts, L Reingold, S Auepemkiat, SP Voravuthik, A Johnston
    PLoS ONE, 2018-10-15;13(10):e0205340.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Placental Ras Regulates Inflammation Associated with Maternal Obesity
    Authors: S Liong, G Barker, M Lappas
    Mediators Inflamm., 2018-10-08;2018(0):3645386.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018-08-15;9(1):3267.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. A bispecific antibody that targets IL-6 receptor and IL-17A for the potential therapy of patients with autoimmune and inflammatory diseases
    Authors: M Lyman, V Lieuw, R Richardson, A Timmer, C Stewart, S Granger, R Woods, M Silacci, D Grabulovsk, R Newman
    J. Biol. Chem., 2018-04-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Pathogenic role of glycan-specific IgG antibodies in IgA nephropathy
    Authors: YF Zhao, L Zhu, LJ Liu, SF Shi, JC Lv, H Zhang
    BMC Nephrol, 2017-09-29;18(1):301.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. The Proangiogenic Capabilities of Malignant Ascites Generated by Aggressive Ovarian Tumors
    Authors: J Miku?a-Pie, P Uruski, S Szubert, K Maksin, R Moszy?ski, D Szpurek, A Wo?niak, S Sajdak, A Tykarski, K Ksi??ek
    Biomed Res Int, 2017-09-20;2017(0):2592496.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017-02-16;65(4):715-729.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. The TRAIL-Induced Cancer Secretome Promotes a Tumor-Supportive Immune Microenvironment via CCR2
    Authors: T Hartwig, A Montinaro, S von Karste, A Sevko, S Surinova, A Chakravart, L Taraborrel, P Draber, E Lafont, F Arce Varga, MA El-Bahrawy, SA Quezada, H Walczak
    Mol. Cell, 2017-02-16;65(4):730-742.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells
    Authors: DM Baston-Bue, O Altergot-A, SJ Pour, JS Krüssel, UR Markert, TN Fehm, AP Bielfeld
    Mediators Inflamm., 2017-02-15;2017(0):8379256.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Serum from Varicose Patients Induces Senescence-Related Dysfunction of Vascular Endothelium Generating Local and Systemic Proinflammatory Conditions
    Oxid Med Cell Longev, 2016-11-23;2016(0):2069290.
    Species: Human
    Sample Types: Serum
  43. Inhibiting complex IL-17A and IL-17RA interactions with a linear peptide
    Sci Rep, 2016-05-17;6(0):26071.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Urinary CXCL1: a novel predictor of IgA nephropathy progression.
    Authors: Zhao, Yanfeng, Zhu, Li, Zhou, Tong, Zhang, Qingxian, Shi, Sufang, Liu, Lijun, Lv, Jicheng, Zhang, Hong
    PLoS ONE, 2015-03-27;10(3):e0119033.
    Species: Human
    Sample Types: Urine
  45. Necroptosis suppresses inflammation via termination of TNF- or LPS-induced cytokine and chemokine production.
    Authors: Kearney C, Cullen S, Tynan G, Henry C, Clancy D, Lavelle E, Martin S
    Cell Death Differ, 2015-01-23;22(8):1313-27.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.
    Authors: Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A
    PLoS ONE, 2015-01-15;10(1):e0116775.
    Species: Human
    Sample Types: BALF
  47. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014-05-12;9(5):e96925.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Chinese herbal medicine suppresses invasion-promoting capacity of cancer-associated fibroblasts in pancreatic cancer.
    Authors: Chen, Lianyu, Qu, Chao, Chen, Hao, Xu, Litao, Qi, Qi, Luo, Jianmin, Wang, Kun, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
    PLoS ONE, 2014-04-29;9(4):e96177.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Roles for the mitogen-activated protein kinase (MAPK) phosphatase, DUSP1, in feedback control of inflammatory gene expression and repression by dexamethasone.
    Authors: Shah S, King E, Chandrasekhar A, Newton R
    J Biol Chem, 2014-04-01;289(19):13667-79.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways.
    Authors: Lo H, Lai T, Li C, Wu W
    Acta Pharmacol Sin, 2014-02-03;35(3):339-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
    Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
    PLoS ONE, 2013-12-30;8(12):e82867.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 2011-12-21;5(2):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
    Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
    J. Biol. Chem., 2010-11-12;286(4):2636-47.
    Species: Human
    Sample Types: Cell Lysates
  54. Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines.
    Authors: Kisand K, Boe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Strobel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A
    J. Exp. Med., 2010-02-01;207(2):299-308.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. The inflammatory microenvironment of the aging prostate facilitates cellular proliferation and hypertrophy.
    Authors: Begley LA, Kasina S, Macdonald J, Macoska JA
    Cytokine, 2008-06-24;43(2):194-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Impact of tobacco-smoke on key signaling pathways in the innate immune response in lung macrophages.
    Authors: Birrell MA, Wong S, Catley MC, Belvisi MG
    J. Cell. Physiol., 2008-01-01;214(1):27-37.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses.
    Authors: Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG
    J. Biol. Chem., 2007-08-31;282(44):31882-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
    Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
    J. Pharmacol. Exp. Ther., 2006-05-11;318(2):741-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
  60. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004-03-25;104(2):314-20.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human CXCL1/GRO alpha DuoSet ELISA
By Anonymous on 10/20/2021
Sample Tested: human cell line conditioned media
Human CXCL1/GRO alpha DuoSet ELISA
By Anonymous on 11/25/2020
Sample Tested: cancer cell conditioned media
Human CXCL1/GRO alpha DuoSet ELISA DY275
Human CXCL1/GRO alpha DuoSet ELISA
By Anonymous on 11/14/2019
Sample Tested: Huh-7 human hepatoma cell line

Very sensitive!Perfect!

Human CXCL1/GRO alpha DuoSet ELISA DY275
Human CXCL1/GRO alpha DuoSet ELISA
By Anonymous on 08/08/2019
Sample Tested: Tissue Culture Media
Human CXCL1/GRO alpha DuoSet ELISA DY275