Home / IL-17/IL-17A / Human IL-17 DuoSet ELISA

Human IL-17 DuoSet ELISA

Catalog #: DY317
72 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY317
DY317-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
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Human IL-17 / IL-17A ELISA Standard Curve
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Citations (72)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human IL-17 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-17. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human IL-17 / IL-17A ELISA Standard Curve View Larger

Human IL-17 / IL-17A ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

Human Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a 15-20 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines (1-5). Its alternate name, CTLA-8, originated from rodent studies where an activated hybridoma was created from the fusion of a mouse cytotoxic and a rat T cell lymphoma cell line. The molecule of interest in this study was assumed to have come from the mouse cytotoxic lymphocyte cell (thus the CTL designation), whereas, in fact, it was a rat lymphocyte molecule. Human IL-17/17A is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 133 aa mature region that possesses a cysteine-knot fold (4-6). In both human and mouse, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 32-38 kDa disulfide-linked homodimer, and a 40-45 kDa covalent heterodimer with IL-17F (7-9). Most secreted IL-17A is in the form of the IL-17A:F heterodimer, however, the IL-17A:A homodimer is the most bioactive of the two forms (8). Mature human IL-17A is 61%, 74%, and 99% aa identical to mouse, porcine, and chimpanzee IL-17A, respectively (10-12). Mammalian cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27- gamma δ T cells, CD1+NK1.1- iNKT cells, and CD3- CD4+ LTi-like cells (9, 13-17). 

A high affinity receptor for human IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1, 18). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 15). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (19, 20). Both receptors are needed for IL-17A and IL-17A:F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 21, 22). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (14, 15, 23, 24, 25). Its induction was initially described to be the result of antigen stimulation of dendritic cells, resulting in IL-23 secretion. In a T cell receptor-independent event, IL-23 induces T cell production of IL-17 (14). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and stem cell factor (membrane form), an effect that increases polymorphonuclear neutrophils (PMN) differentiation and production. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating PMN numbers (23). In the tissues, IL-17 would also seem to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha, IL-1b and IL-6 production (26). TNF-alpha and IL-1b then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (27). IL-17 further contributes to PMN influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (14, 28). IL-17 effects are not limited to inflammation. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (24). In conjunction with IL-4 and CD40L, IL-17A also promotes the generation of IgE secreting cells (29). And in white fat, IL-17A inhibits adipocyte differentiation from preadipocytes, and impairs glucose uptake by mature adipocytes (30).

Long Name:
Interleukin 17
Entrez Gene IDs:
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names:
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human IL-17 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

72 Citations: Showing 1 - 10
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  1. Effects of Combinatory In Vitro Treatment with Immune Checkpoint Inhibitors and Cytarabine on the Anti-Cancer Immune Microenvironment in De Novo AML Patients
    Authors: Bo?kun, ?;Starosz, A;Kr?towska-Grunwald, A;Wasiluk, T;Walewska, A;Wierzbowska, A;Moniuszko, M;Grubczak, K;
    Cancers
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Significance of Th17 and Treg in Treatment Efficacy and Outcome in Pediatric Acute Lymphoblastic Leukemia
    Authors: Kr?towska-Grunwald, A;Sawicka-?ukowska, M;Kowalska, M;Basaj, A;Krawczuk-Rybak, M;Moniuszko, M;Grubczak, K;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease
    Authors: Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Effect of exogenous IL-37 on immune cells from a patient carrying a potential IL37 loss-of-function variant: A case study
    Authors: LU Teufel, CI van der Ma, V Klück, A Simons, A Hoischen, V Vernimmen, LAB Joosten, RJW Arts
    Cytokine, 2022-12-05;162(0):156102.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis
    Authors: JS Park, SC Yang, HY Jeong, SY Lee, JG Ryu, JW Choi, HY Kang, SM Kim, SH Hwang, ML Cho, SH Park
    Arthritis Research & Therapy, 2022-11-17;24(1):254.
    Species: Human
    Sample Types: Serum
  6. T Lymphocyte Serotonin 5-HT7 Receptor Is Dysregulated in Natalizumab-Treated Multiple Sclerosis Patients
    Authors: F Reverchon, C Guillard, L Mollet, P Auzou, D Gosset, F Madouri, A Valéry, A Menuet, C Ozsancak, M Pallix-Guy, S Morisset-L
    Biomedicines, 2022-09-27;10(10):.
    Species: Human
    Sample Types: Plasma
  7. Interleukin-17, a salivary biomarker for COVID-19 severity
    Authors: FS Sharif-Ask, NS Sharif-Ask, S Hafezi, B Mdkhana, HAH Alsayed, AW Ansari, B Mahboub, AM Zakeri, MH Temsah, W Zahir, Q Hamid, R Halwani
    PLoS ONE, 2022-09-22;17(9):e0274841.
    Species: Human
    Sample Types: Plasma
  8. Exercise training-induced changes in immunometabolic markers in youth badminton athletes
    Authors: FE Rossi, AJ Maldonado, JM Cholewa, SLG Ribeiro, CA de Araújo, C Figueiredo, T Reichel, K Krüger, FS Lira, LG Minuzzi
    Scientific Reports, 2022-09-15;12(1):15539.
    Species: Human
    Sample Types: Plasma
  9. IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation.
    Authors: Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S
    Int J Mol Sci, 2022-08-29;23(17):.
    Species: Human
    Sample Types: Tissue Culture Supernates
  10. Lithocholic acid inhibits dendritic cell activation by reducing intracellular glutathione via TGR5 signaling
    Authors: J Hu, Y Zhang, S Yi, C Wang, X Huang, S Pan, J Yang, G Yuan, S Tan, H Li
    International journal of biological sciences, 2022-07-11;18(11):4545-4559.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. High-intensity intermittent exercise induces a potential anti-inflammatory response in healthy women across the menstrual cycle
    Authors: LG Minuzzi, FS Lira, RAB de Poli, VH Fialho Lop, AM Zagatto, K Suzuki, BM Antunes
    Cytokine, 2022-04-08;154(0):155872.
    Species: Human
    Sample Types: Serum
  12. A novel Streptococcus pneumoniae human challenge model demonstrates Treg lymphocyte recruitment to the infection site
    Authors: G Szylar, R Wysoczansk, H Marshall, DJB Marks, R José, ME Ehrenstein, JS Brown
    Scientific Reports, 2022-03-07;12(1):3990.
    Species: Human
    Sample Types: Blister Fluid
  13. Vitamin D3 and zinc synergistically induce regulatory T cells and suppress interferon-gamma production in mixed lymphocyte culture
    Authors: AK Schmitt, MA Puppa, I Wessels, L Rink
    The Journal of nutritional biochemistry, 2022-01-19;0(0):108942.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Skin Substitute Preparation Method Induces Immunomodulatory Changes in Co-Incubated Cells through Collagen Modification
    Authors: J Holl, C Pawlukiani, J Corton Rui, D Groth, K Grubczak, HR Hady, J Dadan, J Reszec, S Czaban, C Kowalewski, M Moniuszko, A Eljaszewic
    Pharmaceutics, 2021-12-15;13(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Bacterial genotoxins induce T�cell senescence
    Authors: SL Mathiasen, L Gall-Mas, IS Pateras, SDP Theodorou, MRJ Namini, MB Hansen, OCB Martin, CK Vadivel, K Ntostoglou, D Butter, M Givskov, C Geisler, AN Akbar, VG Gorgoulis, T Frisan, N Ødum, T Krejsgaard
    Cell Reports, 2021-06-08;35(10):109220.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. NLRP1 acts as a negative regulator of Th17 cell programming in mice and humans with autoimmune diabetes
    Authors: FRC Costa, JA Leite, DM Rassi, JF da Silva, J Elias-Oliv, JB Guimarães, MC Foss-Freit, NOS Câmara, A Pontillo, RC Tostes, JS Silva, D Carlos
    Cell Reports, 2021-05-25;35(8):109176.
    Species: Human
    Sample Types: Serum
  17. Higher CD4/CD8 ratio of pleural effusion predicts better survival for lung cancer patients receiving immune checkpoint inhibitors
    Authors: PH Lee, TY Yang, KC Chen, YH Huang, JS Tseng, KH Hsu, YC Wu, KJ Liu, GC Chang
    Scientific Reports, 2021-04-30;11(1):9381.
    Species: Human
    Sample Types: Pleural Effusion
  18. Electrophilic nitro-fatty acids suppress psoriasiform dermatitis: STAT3 inhibition as a contributory mechanism
    Authors: P Wang, ME Killeen, TL Sumpter, LK Ferris, LD Falo, BA Freeman, FJ Schopfer, AR Mathers
    Redox Biology, 2021-04-24;43(0):101987.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. New Biomarker in Chagas Disease: Extracellular Vesicles Isolated from Peripheral Blood in Chronic Chagas Disease Patients Modulate the Human Immune Response
    Authors: RP Madeira, LM Dal'Mas Ro, P de Cássia, C Mady, BM Ianni, AC Torrecilha
    Journal of Immunology Research, 2021-01-11;2021(0):6650670.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. SARS-CoV-2-specific IgA and limited inflammatory cytokines are present in the stool of select patients with acute COVID-19
    Authors: GJ Britton, A Chen-Liaw, F Cossarini, AE Livanos, MP Spindler, T Plitt, J Eggers, I Mogno, A Gonzalez-R, S Siu, M Tankelevic, L Grinspan, RE Dixon, D Jha, G Martinez-D, F Amanat, DA Hoagland, B tenOever, MC Dubinsky, M Merad, H van Bakel, F Krammer, G Bongers, S Mehandru, JJ Faith
    medRxiv : the preprint server for health sciences, 2020-12-09;0(0):.
    Species: Human
    Sample Types: Fecal Supernates
  21. The DHODH Inhibitor PTC299 Arrests SARS-CoV-2 Replication and Suppresses Induction of Inflammatory Cytokines
    Authors: J Luban, R Sattler, E Mühlberger, JD Graci, L Cao, M Weetall, C Trotta, JM Colacino, S Bavari, C Strambio-D, EL Suder, Y Wang, V Soloveva, K Cintron-Lu, NA Naryshkin, M Pykett, EM Welch, K O'Keefe, R Kong, E Goodwin, A Jacobson, S Paessler, S Peltz
    Virus Res, 2020-11-26;0(0):198246.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Monocytes differentiated into macrophages and dendritic cells in the presence of human IFN-&lambda3 or IFN-&lambda4 show distinct phenotypes
    Authors: M De, A Bhushan, S Chinnaswam
    J Leukoc Biol, 2020-11-17;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. The role of EMMPRIN/CD147 in regulating angiogenesis in patients with psoriatic arthritis
    Authors: MA Rahat, M Safieh, E Simanovich, E Pasand, T Gazitt, A Haddad, M Elias, D Zisman
    Arthritis Res Ther, 2020-10-14;22(1):240.
    Species: Human
    Sample Types: Serum
  24. Lung immunoglobulin A immunity dysregulation in cystic fibrosis
    Authors: AM Collin, M Lecocq, S Noel, B Detry, FM Carlier, F Aboubakar, C Bouzin, T Leal, M Vermeersch, V De Rose, L Regard, C Martin, PR Burgel, D Hoton, S Verleden, A Froidure, C Pilette, S Gohy
    EBioMedicine, 2020-09-11;60(0):102974.
    Species: Human
    Sample Types: Sputum Supernates
  25. Erythromycin inhibits neutrophilic inflammation and mucosal disease by upregulating DEL-1
    Authors: T Maekawa, H Tamura, H Domon, T Hiyoshi, T Isono, D Yonezawa, N Hayashi, N Takahashi, K Tabeta, T Maeda, M Oda, A Ziogas, V? Alexaki, T Chavakis, Y Terao, G Hajishenga
    JCI Insight, 2020-08-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. FTY720 ameliorates GvHD by blocking T lymphocyte migration to target organs and by skin fibrosis inhibition
    Authors: J Ryu, J Jhun, MJ Park, JA Baek, SY Kim, KH Cho, JW Choi, SH Park, JY Choi, ML Cho
    J Transl Med, 2020-06-06;18(1):225.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
    Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
    J Immunol Res, 2020-04-21;2020(0):8074183.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. 1,25-dihydroxy Vitamin D3 and Interleukin-6 Blockade Synergistically Regulate Rheumatoid Arthritis by Suppressing Interleukin-17 Production and Osteoclastogenesis
    Authors: H Kim, S Baek, SM Hong, J Lee, SM Jung, J Lee, M Cho, SK Kwok, SH Park
    J. Korean Med. Sci., 2020-02-17;35(6):e40.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Locally-secreted interleukin-6 is related with radiological severity in smear-negative pulmonary tuberculosis
    Authors: PX Losada, F Perdomo-Ce, M Castro, C Salcedo, A Salcedo, I DeLaura, G Lastra, CF Narváez
    Cytokine, 2019-12-25;127(0):154950.
    Species: Human
    Sample Types: BALF
  30. Replication of Genome-Wide Association Analysis Identifies New Susceptibility Loci at Long Noncoding RNA Regions for Vogt-Koyanagi-Harada Disease
    Authors: J Qi, L Du, J Deng, Y Qin, G Su, S Hou, M Lv, Q Zhang, A Kijlstra, P Yang
    Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4820-4829.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Type 1 diabetic mellitus patients with increased atherosclerosis risk display decreased CDKN2A/2B/2BAS gene expression in leukocytes
    Authors: S Martínez-H, V Sánchez-Ga, A Herrero-Ce, Á Vinué, JT Real, JF Ascaso, DJ Burks, H González-N
    J Transl Med, 2019-07-12;17(1):222.
    Species: Human
    Sample Types: Plasma
  32. PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia
    Authors: T Lopatina, E Favaro, C Grange, M Cedrino, A Ranghino, S Occhipinti, S Fallo, F Buffolo, DA Gaykalova, MM Zanone, R Romagnoli, G Camussi
    Sci Rep, 2018-12-04;8(1):17458.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Disabled-2 (DAB2) Overexpression Inhibits Monocyte-Derived Dendritic Cells' Function in Vogt-Koyanagi-Harada Disease
    Authors: S Yi, R Chang, J Hu, Y Qiu, Q Wang, Q Cao, G Yuan, G Su, C Zhou, Y Wang, A Kijlstra, P Yang
    Invest. Ophthalmol. Vis. Sci., 2018-09-04;59(11):4662-4669.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Association of genetic variations in PTPN2 and CD122 with ocular Behcet's disease
    Authors: Q Zhang, H Li, S Hou, H Yu, G Su, B Deng, J Qi, C Zhou, A Kijlstra, P Yang
    Br J Ophthalmol, 2018-03-03;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Cholera toxin enhances IL-17A production in both CD4and CD8cells via a cAMP/PKA-mediated IL-17A promoter activation
    Authors: HC Tsa, S Velichko, S Lee, R Wu
    Immunology, 2018-02-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Angiogenic T cell expansion correlates with severity of peripheral vascular damage in systemic sclerosis
    Authors: M Manetti, S Pratesi, E Romano, S Bellando-R, I Rosa, S Guiducci, BS Fioretto, L Ibba-Manne, E Maggi, M Matucci-Ce
    PLoS ONE, 2017-08-10;12(8):e0183102.
    Species: Human
    Sample Types: Serum
  37. IFN-?1 with Th17 axis cytokines and IFN-? define different subsets in systemic lupus erythematosus (SLE)
    Authors: V Oke, S Brauner, A Larsson, J Gustafsson, A Zickert, I Gunnarsson, E Svenungsso
    Arthritis Res. Ther., 2017-06-15;19(1):139.
    Species: Human
    Sample Types: Serum
  38. TB lymphadenitis is associated with enhanced baseline and antigen - specific induction of Type 1 and Type 17 cytokines and reduced IL-1? and IL-18 at the site of infection
    Authors: GR Kathamuthu, K Moideen, D Baskaran, VV Banurekha, D Nair, G Sekar, R Sridhar, B Vidyajayan, G Gajendrara, DK Parandhama, A Srinivasan, S Babu
    Clin. Vaccine Immunol, 2017-05-05;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. The heterogeneity of systemic inflammation in bronchiectasis
    Authors: AD Saleh, JD Chalmers, A De Soyza, TC Fardon, SO Koustas, J Scott, AJ Simpson, JS Brown, JR Hurst
    Respir Med, 2017-04-17;127(0):33-39.
    Species: Human
    Sample Types: Serum
  40. Presence of IL-17 in synovial fluid identifies a potential inflammatory osteoarthritic phenotype
    Authors: SJ Snelling, S Bas, GJ Puskas, SG Dakin, D Suva, A Finckh, C Gabay, P Hoffmeyer, AJ Carr, A Lübbeke
    PLoS ONE, 2017-04-11;12(4):e0175109.
    Species: Human
    Sample Types: Serum
  41. Berberine exerts an anti-inflammatory role in ocular Behcet's disease
    Mol Med Rep, 2016-12-05;15(1):97-102.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion
    Authors: Mélissa Noack
    Arthritis Res Ther, 2016-06-23;18(0):148.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Analysis of receptor tyrosine kinase genetics identifies two novel risk loci in GAS6 and PROS1 in Beh�et's disease
    Sci Rep, 2016-05-25;6(0):26662.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation
    Authors: A Malecka, Q Wang, S Shah, RV Sutavani, I Spendlove, JM Ramage, J Greensmith, HA Franks, MJ Gough, A Saalbach, PM Patel, AM Jackson
    J Leukoc Biol, 2016-04-05;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Cooperation between IL-7 Receptor and Integrin alpha2beta1 (CD49b) Drives Th17-Mediated Bone Loss.
    Authors: El Azreq M, Arseneault C, Boisvert M, Page N, Allaeys I, Poubelle P, Tessier P, Aoudjit F
    J Immunol, 2015-09-25;195(9):4198-209.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Interleukin-23 Receptor Gene Polymorphism May Enhance Expression of the IL-23 Receptor, IL-17, TNF-alpha and IL-6 in Behcet's Disease.
    Authors: Jiang Z, Hennein L, Tao Y, Tao L
    PLoS ONE, 2015-07-29;10(7):e0134632.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Cytokine profiles at admission can be related to outcome in AIDS patients with cryptococcal meningitis.
    Authors: Mora D, Fortunato L, Andrade-Silva L, Ferreira-Paim K, Rocha I, Vasconcelos R, Silva-Teixeira D, Nascentes G, Silva-Vergara M
    PLoS ONE, 2015-03-23;10(3):e0120297.
    Species: Human
    Sample Types: Serum
  48. A functional variant of PTPN22 confers risk for Vogt-Koyanagi-Harada syndrome but not for ankylosing spondylitis.
    Authors: Zhang Q, Qi J, Hou S, DU L, Yu H, Cao Q, Zhou Y, Liao D, Kijlstra A, Yang P
    PLoS ONE, 2014-05-09;9(5):e96943.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. The role of interleukin-1 receptor-associated kinases in Vogt-Koyanagi-Harada disease.
    Authors: Sun M, Yang P, DU L, Yang Y, Ye J
    PLoS ONE, 2014-04-01;9(4):e93214.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. HIF-1alpha- and hypoxia-dependent immune responses in human CD4+CD25high T cells and T helper 17 cells.
    Authors: Bollinger T, Gies S, Naujoks J, Feldhoff L, Bollinger A, Solbach W, Rupp J
    J Leukoc Biol, 2014-03-24;96(2):305-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels.
    Authors: Arndts, Kathrin, Specht, Sabine, Debrah, Alexande, Tamarozzi, Francesc, Klarmann Schulz, Ute, Mand, Sabine, Batsa, Linda, Kwarteng, Alexande, Taylor, Mark, Adjei, Ohene, Martin, Coralie, Layland, Laura E, Hoerauf, Achim
    PLoS Negl Trop Dis, 2014-02-20;8(2):e2679.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014-02-18;44(5):1330-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. IFN- alpha blocks IL-17 production by peripheral blood mononuclear cells in patients with chronic active hepatitis B Infection.
    Authors: Cui F, Meng J, Luo P, Chen P
    BMC Infect Dis, 2014-02-01;14(0):55.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells.
    Authors: Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H
    Br J Haematol, 2014-01-15;165(1):134-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
    Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
    Nat Commun, 2014-01-01;5(0):3199.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Independent and interdependent immunoregulatory effects of IL-27, IFN-beta, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis.
    Authors: Fitzgerald D, Fonseca-Kelly Z, Cullimore M, Safabakhsh P, Saris C, Zhang G, Rostami A
    J Immunol, 2013-03-01;190(7):3225-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. CCR2+CCR5+ T Cells Produce Matrix Metalloproteinase-9 and Osteopontin in the Pathogenesis of Multiple Sclerosis.
    Authors: Sato W, Tomita A, Ichikawa D, Lin Y, Kishida H, Miyake S, Ogawa M, Okamoto T, Murata M, Kuroiwa Y, Aranami T, Yamamura T
    J Immunol, 2012-10-15;189(10):5057-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti.
    Authors: Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A
    PLoS Negl Trop Dis, 2012-04-03;6(4):e1611.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
    Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
    Proc. Natl. Acad. Sci. U.S.A., 2012-02-06;109(8):3001-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease.
    Authors: Chu M, Yang P, Hu R, Hou S, Li F, Chen Y, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2011-09-09;52(10):7084-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.
    Authors: Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y
    Mol. Vis., 2011-05-31;17(0):1425-30.
    Species: Human
    Sample Types: Serum
  62. IL-27 mediates the response to IFN-beta therapy in multiple sclerosis patients by inhibiting Th17 cells.
    Authors: Sweeney CM, Lonergan R, Basdeo SA, Kinsella K, Dungan LS, Higgins SC, Kelly PJ, Costelloe L, Tubridy N, Mills KH, Fletcher JM
    Brain Behav. Immun., 2011-03-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  63. A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
    Authors: Yu RY, Gallagher G
    J. Immunol., 2010-11-12;185(12):7302-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. IFN-alpha blocks IL-17 production by peripheral blood mononuclear cells in Behcet's disease.
    Authors: Liu X, Yang P, Wang C, Li F, Kijlstra A
    Rheumatology (Oxford), 2010-11-08;50(2):293-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2010-09-08;25(1):409-19.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009-05-01;39(5):1221-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009-03-02;119(10):1424-32.
    Species: Human
    Sample Types: Plasma
  68. Characterization of the interleukin-17 isoforms and receptors in lesional psoriatic skin.
    Authors: Johansen C, Usher PA, Kjellerup RB
    Br. J. Dermatol., 2008-10-21;160(2):319-24.
    Species: Human
    Sample Types: Tissue Homogenates
  69. Diminished frequency and function of CD4+CD25high regulatory T cells associated with active uveitis in Vogt-Koyanagi-Harada syndrome.
    Authors: Chen L, Yang P, Zhou H, He H, Ren X, Chi W, Wang L, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2008-04-17;49(8):3475-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Cutting edge: Human Th17 cells are identified as bearing CCR2+CCR5- phenotype.
    Authors: Sato W, Aranami T, Yamamura T
    J. Immunol., 2007-06-15;178(12):7525-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC.
    Authors: Meyers JA, Mangini AJ, Nagai T, Roff CF, Sehy D, van Seventer JM
    Cytokine, 2006-10-18;35(5):235-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004-07-15;173(2):1463-76.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human IL-17 DuoSet ELISA
By Anonymous on 11/15/2022
Sample Tested: Serum
Human IL-17 DuoSet ELISA
By James Williams on 03/16/2022
Sample Tested: Sputum

Easy to use protocol and great results

Human IL-17 DuoSet ELISA
By Sophie Millar on 01/16/2019
Sample Tested: Cell Culture Media
Human IL-17 DuoSet ELISA DY317