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Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

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Human IL-1 beta/IL-1F2 ELISA Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (10 uL)
Sensitivity
0.063 pg/mL
Assay Range
0.1 - 8 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human IL-1 beta. This assay also recognizes recombinant rhesus macaque IL-1 beta (aa 117-269).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine HS Human IL-1 beta Immunoassay is a 4 hour solid phase ELISA designed to measure human IL-1 beta levels in serum, plasma, and saliva. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1 beta. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural human IL-1 beta. Reports indicate that ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in serum, plasma, and saliva.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 0.315 1.3 2.52 0.27 1.36 2.6
Standard Deviation 0.014 0.05 0.06 0.029 0.101 0.163
CV% 4.4 3.9 2.4 10.7 7.4 6.3

Recovery

The recovery of human IL-1 beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 94 87-106
Heparin Plasma (n=4) 92 89-99
Serum (n=4) 91 86-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-1 beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IL-1 beta/IL-1F2 ELISA Linearity
Human IL-1 beta/IL-1F2 High Sensitivity ELISA Linearity

Scientific Data

N/A Human IL-1 beta/IL-1F2 ELISA Standard Curve View Larger

Human IL-1 beta/IL-1F2 ELISA Standard Curve

N/A Human IL-1 beta/IL-1F2 High Sensitivity Standard Curve View Larger

Human IL-1 beta/IL-1F2 High Sensitivity Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

The Interleukin 1 (IL-1) family of proteins consists of the classic members IL-1 alpha, IL-1 beta, and IL-1ra, plus IL-18, IL-33 and IL-1F5-F10. IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions. IL-1 is not produced by unstimulated cells of healthy individuals with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. However, in response to inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is observed. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. 


 IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into mature proteins of approximately 17.5 kDa. Cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. Neither IL-1 alpha nor IL-1 beta contains a typical hydrophobic signal peptide, but evidence suggests that these factors can be secreted by non-classical pathways. A portion of unprocessed IL-1 alpha can be presented on the cell membrane and may retain biological activity. The precursor form of IL-1 beta, unlike the IL-1 alpha precursor, shows little or no biological activity in comparison to the processed form. Both unprocessed and mature forms of IL-1 beta are exported from the cell. 

 IL-1 alpha and IL-1 beta exert their effects through immunoglobulin superfamily receptors that additionally bind IL-1ra. The 80 kDa transmembrane type I receptor (IL-1 RI) is expressed on T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes. The 68 kDa transmembrane type II receptor (IL-1 RII) is expressed on B cells, neutrophils, and bone marrow cells. The two IL-1 receptor types show approximately 28% homology in their extracellular domains but differ significantly in that the type II receptor has a cytoplasmic domain of only 29 amino acids (aa), whereas the type I receptor has a 213 aa cytoplasmic domain. IL-1 RII does not appear to signal in response to IL-1 and may function as a decoy receptor that attenuates IL-1 function. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction. IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1. Soluble forms of both IL-1 RI and IL-1 RII have been detected in human plasma, synovial fluids, and the conditioned media of several human cell lines. In addition, IL-1 binding proteins that resemble soluble IL-1 RII are encoded by vaccinia and cowpox viruses.

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

75 Citations: Showing 1 - 10
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  1. IL-10 and IL-1? Serum Levels, Genetic Variants, and Metabolic Syndrome: Insights into Older Adults' Clinical Characteristics
    Authors: Freitas, RS;de Souza Silva, CM;Ferreira Fratelli, C;Ramos de Lima, L;Morato Stival, M;Schwerz Funghetto, S;Rodrigues da Silva, IC;Vieira de Andrade, R;
    Nutrients
    Species: Human hepegivirus
    Sample Types: Serum
  2. An IL-1?-driven neutrophil-stromal cell axis fosters a BAFF-rich protumor microenvironment in individuals with multiple myeloma
    Authors: de Jong, MME;Fokkema, C;Papazian, N;Czeti, Á;Appelman, MK;Vermeulen, M;van Heusden, T;Hoogenboezem, RM;van Beek, G;Tahri, S;Sanders, MA;van de Woestijne, PC;Gay, F;Moreau, P;Büttner-Herold, M;Bruns, H;van Duin, M;Broijl, A;Sonneveld, P;Cupedo, T;
    Nature immunology
    Species: Human hepegivirus
    Sample Types: Bone Marrow Plasma
  3. Cell-free fat extract promotes axon regeneration and retinal ganglion cells survival in traumatic optic neuropathy
    Authors: Sun, Y;Chen, D;Dai, T;Yu, Z;Xie, H;Wang, X;Zhang, W;
    Frontiers in cellular neuroscience
    Species: Human hepegivirus
    Sample Types: Cell Extracts
  4. HIV Infection Drives Foam Cell Formation via NLRP3 Inflammasome Activation
    Authors: Caocci, M;Niu, M;Fox, HS;Burdo, TH;
    International journal of molecular sciences
    Species: Human hepegivirus
    Sample Types: Cell Culture Supernates
  5. Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
    Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
    Cell reports
    Species: Human hepegivirus
    Sample Types: Ascites Fluid
  6. Interleukin-19 Levels Are Increased in Palmoplantar Pustulosis and Reduced following Apremilast Treatment
    Authors: K Wolk, D Wilsmann-T, K Witte, TC Brembach, C Kromer, S Gerdes, K Ghoreschi, K Reich, R Mössner, R Sabat
    International Journal of Molecular Sciences, 2023-01-09;24(2):.
    Species: Human
    Sample Types: Plasma
  7. Infection and Persistence of Coxiella burnetii Clinical Isolate in the Placental Environment
    Authors: SM Zarza, M Militello, L Gay, A Levasseur, H Lepidi, Y Bechah, S Mezouar, JL Mege
    International Journal of Molecular Sciences, 2023-01-07;24(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. A comprehensive approach to predicting weight gain and therapy response in psychopharmacologically treated major depressed patients: A cohort study protocol
    Authors: MS Simon, BB Barton, C Glocker, R Musil
    PLoS ONE, 2022-07-21;17(7):e0271793.
    Species: Human
    Sample Types: Plasma
  9. Suppressors of Cytokine Signaling Are Decreased in Major Depressive Disorder Patients
    Authors: N Kobayashi, S Shinagawa, T Nagata, M Shigeta, K Kondo
    Journal of personalized medicine, 2022-06-25;12(7):.
    Species: Human
    Sample Types: Serum
  10. Wheat Biscuits Enriched with Plant-Based Protein Contribute to Weight Loss and Beneficial Metabolic Effects in Subjects with Overweight/Obesity
    Authors: P Binou, AE Yanni, K Kartsioti, A Barmpagian, P Konstantop, VT Karathanos, A Kokkinos
    Nutrients, 2022-06-17;14(12):.
    Species: Human
    Sample Types: Plasma
  11. Nicotine regulates autophagy of human periodontal ligament cells through alpha7 nAchR that promotes secretion of inflammatory factors IL-1beta and IL-8
    Authors: Y Du, K Yang, Z Zhou, L Wu, L Wang, Y Chen, X Ge, X Wang
    BMC Oral Health, 2021-11-03;21(1):560.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Impact of mind-body intervention on proinflammatory cytokines interleukin 6 and 1beta: A three-arm randomized controlled trial for persons with sleep disturbance and depression
    Authors: SM Ng, MXC Yin, JSM Chan, CHY Chan, TCT Fong, A Li, KF So, LP Yuen, JP Chen, KF Chung, CLW Chan
    Brain, Behavior, and Immunity, 2021-10-08;99(0):166-176.
    Species: Human
    Sample Types: Plasma
  13. The proton ATPase inhibitor bafilomycin A1 reduces the release of rhinovirus C and cytokines from primary cultures of human nasal epithelial cells
    Authors: M Yamaya, X Deng, A Kikuchi, M Sugawara, N Saito, T Kubo, H Momma, T Kawase, K Nakagome, Y Shimotai, H Nishimura
    Virus research, 2021-08-21;304(0):198548.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men
    Authors: M Wiecek, Z Szygula, J Gradek, J Kusmierczy, J Szymura
    Biomolecules, 2021-07-16;11(7):.
    Species: Human
    Sample Types: Plasma
  15. The multiple myeloma microenvironment is defined by an inflammatory stromal cell landscape
    Authors: MME de Jong, Z Kellermaye, N Papazian, S Tahri, D Hofste Op, R Hoogenboez, MA Sanders, PC van de Woe, PK Bos, C Khandanpou, J Vermeulen, P Moreau, M van Duin, A Broijl, P Sonneveld, T Cupedo
    Nature Immunology, 2021-05-20;22(6):769-780.
    Species: Human
    Sample Types: Plasma
  16. Comparative evaluation of inflammatory parameters and substitute insulin resistance indices in elderly women with and without type 2 diabetes mellitus
    Authors: MM Leite, MT Dutra, MVG da Costa, SS Funghetto, AO Silva, LR de Lima, ICR da Silva, MR Mota, MM Stival
    Experimental gerontology, 2021-05-03;150(0):111389.
    Species: Human
    Sample Types: Plasma
  17. Macrophage migration inhibitory factor may play a protective role in osteoarthritis
    Authors: M Liu, Z Xie, G Sun, L Chen, D Qi, H Zhang, J Xiong, A Furey, P Rahman, G Lei, G Zhai
    Arthritis Research & Therapy, 2021-02-20;23(1):59.
    Species: Human
    Sample Types: Plasma
  18. Effects of brief stress management interventions on distress and leukocyte nuclear factor kappa B expression during primary treatment for breast cancer: A randomized trial
    Authors: A Diaz, CJ Taub, ME Lippman, MH Antoni, BB Blomberg
    Psychoneuroendocrinology, 2021-02-04;126(0):105163.
    Species: Human
    Sample Types: Serum
  19. Comparison of two lipid emulsions on interleukin-1&beta, interleukin-8 and fatty acid composition in infants post gastrointestinal surgery:�a randomized trial
    Authors: MH Hanindita, R Irawan, IDG Ugrasena, IGBA Hariastawa
    F1000Research, 2020-09-24;9(0):1168.
    Species: Human
    Sample Types: Serum
  20. The intestinal microbiome is a co-determinant of the postprandial plasma glucose response
    Authors: NB Søndertoft, JK Vogt, M Arumugam, M Kristensen, RJ Gøbel, Y Fan, L Lyu, MI Bahl, C Eriksen, L Ängquist, H Frøkiær, TH Hansen, S Brix, HB Nielsen, T Hansen, H Vestergaar, R Gupta, TR Licht, L Lauritzen, O Pedersen
    PLoS ONE, 2020-09-18;15(9):e0238648.
    Species: Human
    Sample Types: Serum
  21. Interferon and anti-TNF therapies differentially modulate amygdala reactivity which predicts associated bidirectional changes in depressive symptoms
    Authors: KA Davies, E Cooper, V Voon, J Tibble, M Cercignani, NA Harrison
    Mol. Psychiatry, 2020-05-26;0(0):.
    Species: Human
    Sample Types: Plasma
  22. Trust as a mediator in the relationship between childhood sexual abuse and IL-6 level in adulthood
    Authors: SM Ng, LL Leng, QW Xie, JSM Chan, CHY Chan, KF So, A Li, KKT Po, LP Yuen, KS Ku, AWM Choi, Z Chouliara, ACY Cheung, CLW Chan, C Emery
    PLoS ONE, 2020-05-15;15(5):e0232932.
    Species: Human
    Sample Types: Plasma
  23. SGLT2 inhibition modulates NLRP3 inflammasome activity via ketones and insulin in diabetes with cardiovascular disease
    Authors: SR Kim, SG Lee, SH Kim, JH Kim, E Choi, W Cho, JH Rim, I Hwang, CJ Lee, M Lee, CM Oh, JY Jeon, HY Gee, JH Kim, BW Lee, ES Kang, BS Cha, MS Lee, JW Yu, JW Cho, JS Kim, YH Lee
    Nat Commun, 2020-05-01;11(1):2127.
    Species: Human
    Sample Types: Serum
  24. Effects of an 8-Week Protein Supplementation Regimen with Hyperimmunized Cow Milk on Exercise-Induced Organ Damage and Inflammation in Male Runners: A Randomized, Placebo Controlled, Cross-Over Study
    Authors: S Ma, T Tominaga, K Kanda, K Sugama, C Omae, S Hashimoto, K Aoyama, Y Yoshikai, K Suzuki
    Biomedicines, 2020-03-04;8(3):.
    Species: Human
    Sample Types: Urine
  25. Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis
    Authors: TH Hung, SF Chen, CH Wu, CC Kao, CP Wu
    Mediators Inflamm., 2019-12-05;2019(0):8687120.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests
    Authors: N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch
    BMC Immunol., 2019-09-14;20(1):33.
    Species: Human
    Sample Types: Serum
  27. LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy
    Authors: J Doll, A Moghaddam, V Daniel, B Biglari, R Heller, G Schmidmaie, TF Raven
    J Orthop, 2019-08-13;17(0):208-214.
    Species: Human
    Sample Types: Serum
  28. Inflammasome-Driven Interleukin-1α and�Interleukin-1? Production in Atherosclerotic Plaques Relates to Hyperlipidemia and Plaque Complexity
    Authors: X Jiang, F Wang, Y Wang, A Gisterå, J Roy, G Paulsson-B, U Hedin, A Lerman, GK Hansson, J Herrmann, ZQ Yan
    JACC Basic Transl Sci, 2019-06-24;4(3):304-317.
    Species: Human
    Sample Types: Tissue Culture Supernates
  29. Galectin 3 and Galectin 3 Binding Protein Improve the Risk Stratification after Myocardial Infarction
    Authors: G Gagno, L Padoan, E Stenner, A Beleù, F Ziberna, C Hiche, A Paldino, G Barbati, G Biolo, N Fiotti, T Not, AP Beltrami, G Sinagra, A Aleksova
    J Clin Med, 2019-04-26;8(5):.
    Species: Human
    Sample Types: Plasma
  30. Inter-ictal assay of peripheral circulating inflammatory mediators in migraine patients under adjunctive cervical non-invasive vagus nerve stimulation (nVNS): A proof-of-concept study
    Authors: SR Chaudhry, IS Lendvai, S Muhammad, P Westhofen, J Kruppenbac, L Scheef, H Boecker, D Scheele, R Hurlemann, TM Kinfe
    Brain Stimul, 2019-01-19;0(0):.
    Species: Human
    Sample Types: Serum
  31. Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
    Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
    J Clin Med, 2018-11-08;7(11):.
    Species: Human
    Sample Types: Plasma
  32. Probiotic Lactobacillus Plantarum 299v decreases kynurenine concentration and improves cognitive functions in patients with major depression: A double-blind, randomized, placebo controlled study
    Authors: L Rudzki, L Ostrowska, D Pawlak, A Ma?us, K Pawlak, N Waszkiewic, A Szulc
    Psychoneuroendocrinology, 2018-10-16;100(0):213-222.
    Species: Human
    Sample Types: Plasma
  33. Association between baseline pro-inflammatory cytokines and brain activation during social exclusion in patients with vulnerability to suicide and depressive disorder
    Authors: I Conejero, I Jaussent, A Cazals, E Thouvenot, T Mura, E Le Bars, S Guillaume, S Squalli, P Courtet, E Olié
    Psychoneuroendocrinology, 2018-10-04;99(0):236-242.
    Species: Human
    Sample Types: Serum
  34. Prolonged mechanical ventilation worsens sepsis-induced diaphragmatic dysfunction in the rat
    Authors: M Le Dinh, S Carreira, J Obert, G Gayan-Rami, B Riou, M Beuvin, T Similowski, C Coirault, A Demoule
    PLoS ONE, 2018-08-01;13(8):e0200429.
    Species: Human
    Sample Types: Plasma
  35. Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression
    Authors: M Hollborn, C Ackmann, H Kuhrt, F Doktor, L Kohen, P Wiedemann, A Bringmann
    Mol. Vis., 2018-07-28;24(0):518-535.
    Species: Human
    Sample Types: Cell Lysates
  36. Acute effect of different concentrations of cayenne pepper cataplasm on sensory-motor functions and serum levels of inflammation-related biomarkers in healthy subjects
    Authors: N Sarabon, S Löfler, J Cvecka, W Hübl, S Zampieri
    Eur J Transl Myol, 2018-03-01;28(1):7333.
    Species: Human
    Sample Types: Serum
  37. Effects of the Ketogenic diet in overweight divers breathing Enriched Air Nitrox
    Authors: G Bosco, A Rizzato, S Quartesan, E Camporesi, D Mangar, M Paganini, L Cenci, S Malacrida, S Mrakic-Spo, S Moretti, A Paoli
    Sci Rep, 2018-02-08;8(1):2655.
    Species: Human
    Sample Types: Plasma
  38. Hemolysis and Inflammatory Response to Extracorporeal Circulation during On-Pump CABG: Comparison between Roller and Centrifugal Pump Systems
    Authors: AC Passaroni, ML Felicio, NLKL Campos, MAM Silva, WB Yoshida
    Braz J Cardiovasc Surg, 2018-01-01;33(1):64-71.
    Species: Human
    Sample Types: Plasma
  39. Interleukin-1? levels predict long-term mortality and need for heart transplantation in ambulatory patients affected by idiopathic dilated cardiomyopathy
    Authors: A Aleksova, AP Beltrami, C Carriere, G Barbati, P Lesizza, M Perrieri-M, M Isola, P Gentile, E Salvioni, T Not, P Agostoni, G Sinagra
    Oncotarget, 2017-04-11;8(15):25131-25140.
    Species: Human
    Sample Types: Plasma
  40. Effect of combined consumption of Lactobacillus brevis KB290 and ?-carotene on minor diarrhoea-predominant irritable bowel syndrome-like symptoms in healthy subjects: a randomised, double-blind, placebo-controlled, parallel-group trial
    Authors: N Fuke, K Aizawa, H Suganuma, T Takagi, Y Naito
    Int J Food Sci Nutr, 2017-04-09;0(0):1-14.
    Species: Human
    Sample Types: Serum
  41. Inflammation and Arterial Stiffness in Chronic Kidney Disease: Findings From the CRIC Study
    Authors: E Peyster, J Chen, HI Feldman, AS Go, J Gupta, N Mitra, Q Pan, A Porter, M Rahman, D Raj, M Reilly, MR Wing, W Yang, RR Townsend
    Am. J. Hypertens., 2017-04-01;30(4):400-408.
    Species: Human
    Sample Types: Plasma
  42. Ghrelin and hormonal markers under exercise training in patients with heart failure with preserved ejection fraction: results from the Ex-DHF pilot study
    Authors: TD Trippel, V Holzendorf, M Halle, G Gelbrich, K Nolte, A Duvinage, S Schwarz, T Rutscher, J Wiora, R Wachter, C Herrmann-L, HD Duengen, G Hasenfu beta, B Pieske, F Edelmann
    ESC Heart Fail, 2016-08-31;4(1):56-65.
    Species: Human
    Sample Types: Serum
  43. Aberrant Inflammasome Activation Characterizes Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome
    Authors: HY Tan, YK Yong, EM Shankar, G Paukovics, R Ellegård, M Larsson, A Kamarulzam, MA French, SM Crowe
    J Immunol, 2016-04-13;0(0):.
    Species: Human
    Sample Types: Plasma
  44. Effects of Acute Endurance Exercise Performed in the Morning and Evening on Inflammatory Cytokine and Metabolic Hormone Responses.
    Authors: Kim H, Konishi M, Takahashi M, Tabata H, Endo N, Numao S, Lee S, Kim Y, Suzuki K, Sakamoto S
    PLoS ONE, 2015-09-09;10(9):e0137567.
    Species: Human
    Sample Types: Plasma
  45. Association between Inflammation and Cardiac Geometry in Chronic Kidney Disease: Findings from the CRIC Study.
    Authors: Gupta J, Dominic E, Fink J, Ojo A, Barrows I, Reilly M, Townsend R, Joffe M, Rosas S, Wolman M, Patel S, Keane M, Feldman H, Kusek J, Raj D
    PLoS ONE, 2015-04-24;10(4):e0124772.
    Species: Human
    Sample Types: Plasma
  46. Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease.
    Authors: Singh R, Mackay A, Patel A, Garcha D, Kowlessar B, Brill S, Donnelly L, Barnes P, Donaldson G, Wedzicha J
    Respir Res, 2014-09-14;15(0):114.
    Species: Human
    Sample Types: Sputum
  47. High-salt intake induced visceral adipose tissue hypoxia and its association with circulating monocyte subsets in humans.
    Authors: Zhou X, Yuan F, Ji W, Guo Z, Zhang L, Lu R, Liu X, Liu H, Zhang W, Jiang T, Zhang Z, Li Y
    Obesity (Silver Spring), 2014-02-19;22(6):1470-6.
    Species: Human
    Sample Types: Serum
  48. Endothelial activation by platelets from sickle cell anemia patients.
    Authors: Proenca-Ferreira R, Brugnerotto A, Garrido V, Dominical V, Vital D, Ribeiro M, dos Santos M, Traina F, Olalla-Saad S, Costa F, Conran N
    PLoS ONE, 2014-02-13;9(2):e89012.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for "inflamm-aging"
    Authors: Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, Benatti S, Gibellini L, Cotichini R, Stazi M, Trenti T, Franceschi C, Cossarizza A
    Eur J Immunol, 2014-02-13;44(5):1552-62.
    Species: Human
    Sample Types: Plasma
  50. Race modifies the association between adiposity and inflammation in patients with chronic kidney disease: findings from the chronic renal insufficiency cohort study.
    Authors: Wing M, Yang W, Teal V, Navaneethan S, Tao K, Ojo A, Guzman N, Reilly M, Wolman M, Rosas S, Cuevas M, Fischer M, Lustigova E, Master S, Xie D, Appleby D, Joffe M, Kusek J, Feldman H, Raj D
    Obesity (Silver Spring), 2014-02-11;22(5):1359-66.
    Species: Human
    Sample Types: Plasma
  51. Echocardiography, spirometry, and systemic acute-phase inflammatory proteins in smokers with COPD or CHF: an observational study.
    Authors: Beghe B, Verduri A, Bottazzi B, Stendardo M, Fucili A, Balduzzi S, Leuzzi C, Papi A, Mantovani A, Fabbri L, Ceconi C, Boschetto P
    PLoS ONE, 2013-11-11;8(11):e80166.
    Species: Human
    Sample Types: Plasma
  52. An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
    Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
    J Neuroinflammation, 2013-02-21;10(0):29.
    Species: Human
    Sample Types: Serum
  53. The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
    Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
    BMC Pulm Med, 2012-02-14;12(0):3.
    Species: Human
    Sample Types: Plasma
  54. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
    Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
    PLoS ONE, 2011-07-28;6(7):e22748.
    Species: Human
    Sample Types: Plasma
  55. Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes.
    Authors: Verma S, Kumar M, Nerurkar VR
    J. Gen. Virol., 2010-11-24;92(0):507-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis.
    Authors: Antonelli A, Ferri C, Ferrari SM, De Marco S, Di Domenicantonio A, Centanni M, Pupilli C, Villa E, Menichetti F, Fallahi P
    J. Interferon Cytokine Res., 2010-10-07;30(11):835-42.
    Species: Human
    Sample Types: Serum
  57. Folic acid treatment normalizes NOS-dependence of vascular tone in the metabolic syndrome.
    Authors: Schneider MP, Schlaich MP, Harazny JM, Raff U, Ritt M, Ott C, Schmieder RE
    Obesity (Silver Spring), 2010-09-23;19(5):960-7.
    Species: Human
    Sample Types: Plasma
  58. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
    Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
    Atherosclerosis, 2009-12-02;210(2):536-41.
    Species: Human
    Sample Types: Plasma
  59. Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
    Authors: Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D
    Inflamm. Res., 2009-10-06;59(5):339-47.
    Species: Human
    Sample Types: Serum
  60. The proinflammatory environment in potential heart and lung donors: prevalence and impact of donor management and hormonal therapy.
    Authors: Venkateswaran RV, Dronavalli V, Lambert PA, Steeds RP, Wilson IC, Thompson RD, Mascaro JG, Bonser RS
    Transplantation, 2009-08-27;88(4):582-8.
    Species: Human
    Sample Types: Serum
  61. Positive affect and inflammation during radiation treatment for breast and prostate cancer.
    Authors: Sepah SC, Bower JE
    Brain Behav. Immun., 2009-06-25;23(8):1068-72.
    Species: Human
    Sample Types: Serum
  62. Maternal IL-1beta production prevents lung injury in a mouse model of bronchopulmonary dysplasia.
    Authors: Backstrom E, Lappalainen U, Bry K
    Am. J. Respir. Cell Mol. Biol., 2009-05-01;42(2):149-60.
    Species: Human
    Sample Types: Plasma
  63. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
    Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
    Cancer Lett., 2009-04-17;283(1):29-35.
    Species: Human
    Sample Types: Serum
  64. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
    Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
    J. Exp. Med., 2009-04-13;206(5):1029-36.
    Species: Human
    Sample Types: Plasma
  65. Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
    Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
    J. Cardiothorac. Vasc. Anesth., 2008-10-22;23(3):316-23.
  66. Correlation between salivary IL-1beta levels and periodontal clinical status.
    Authors: Tobon-Arroyave S, Jaramillo-Gonzalez P, Isaza-Guzman D
    Arch Oral Biol, 2007-12-21;53(4):346-52.
    Species: Human
    Sample Types: Saliva
  67. The influence of anger expression on wound healing.
    Authors: Gouin JP, Kiecolt-Glaser JK, Malarkey WB, Glaser R
    Brain Behav. Immun., 2007-12-19;22(5):699-708.
    Species: Human
    Sample Types: Tissue Secretion
  68. Defining cancer cachexia in head and neck squamous cell carcinoma.
    Authors: Richey LM, George JR, Couch ME, Kanapkey BK, Yin X, Cannon T, Stewart PW, Weissler MC, Shores CG
    Clin. Cancer Res., 2007-11-15;13(22):6561-7.
    Species: Human
    Sample Types: Serum
  69. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006-01-15;12(2):376-82.
    Species: Human
    Sample Types: Plasma
  70. Increased interleukin-18 in patients with juvenile idiopathic arthritis and early attachment loss.
    Authors: Miranda LA, Fischer RG, Sztajnbok FR, Johansson A, Figueredo CM, Gustafsson A
    J. Periodontol., 2005-01-01;76(1):75-82.
    Species: Human
    Sample Types: Serum
  71. Markers of inflammation in exhaled breath condensate of young healthy smokers.
    Authors: Garey KW, Neuhauser MM, Robbins RA, Danziger LH, Rubinstein I
    Chest, 2004-01-01;125(1):22-6.
    Species: Human
    Sample Types: Exhaled Breath Condensate (EBC)
  72. Pathogenesis of Ebola hemorrhagic fever in cynomolgus macaques: evidence that dendritic cells are early and sustained targets of infection.
    Authors: Geisbert TW, Hensley LE, Larsen T, Young HA, Reed DS, Geisbert JB, Scott DP, Kagan E, Jahrling PB, Davis KJ
    Am. J. Pathol., 2003-12-01;163(6):2347-70.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Serum
  73. The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action.
    Authors: Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE
    Immunity, 2003-01-01;18(1):87-96.
    Species: Human
    Sample Types: Serum
  74. Impact of an exercise-induced increase in cardiac troponin I in chronic heart failure secondary to ischemic or idiopathic dilated cardiomyopathy.
    Authors: Schulz O, 2019, Sigusch HH
    e0216744, 2002-09-01;90(5):547-50.
    Species: Human
    Sample Types: Serum
  75. Cytokine production in rhesus monkeys infected with Plasmodium coatneyi.
    Authors: Yang C, Xiao L, Tongren JE, Sullivan J, Lal AA, Collins WE
    Am. J. Trop. Med. Hyg., 1999-08-01;61(2):226-9.
    Species: Primate
    Sample Types: Serum

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Reviews for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

Average Rating: 4.7 (Based on 7 Reviews)

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Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Anonymous on 11/13/2020
Sample Tested: Serum

Standards all the wells in 1 and 2.
R&D QC Low in well 3A and R&D QC High in well 12H.
Rest are serum samples.

Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Anonymous on 11/09/2020
Sample Tested: EDTA Plasma

Good standard curve signal and clear protocol.

Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Lindsie Morin on 10/27/2020
Sample Tested: Serum

Great assay with very easy to follow protocol.

Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Anonymous on 01/30/2020
Sample Tested: Plasma

Easy to use, good results!

Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Evan Williams on 01/30/2020
Sample Tested: Purified Standard
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Anonymous on 01/20/2020
Sample Tested: Serum and Plasma
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Anonymous on 08/04/2017
Sample Tested: Peripheral blood neutrophils

100uL of sample (neat for my experiments) is required per well. Easy to follow instructions.

Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit HSLB00D