Human CCL5/RANTES Quantikine ELISA Kit Summary
Sample Values
Serum/Platelet-poor Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human RANTES in this assay. No medical histories were available for the donors used in this study.Sample Type | Mean (pg/mL) | % Detectable | Range (pg/mL) |
Serum (n=11) | 57,270 | 100% | 23,117-77,354 |
Platelet-poor EDTA plasma (n=11) | 849 | 100% | 262-1587 |
Platelet-poor heparin plasma (n=11) | 1340 | 100% | 308-3527 |
Platelet-poor citrate plasma (n=11) | 467 | 100% | 202-874 |
Urine (n=10) | 656 | 80% | ND-1540 |
Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
Unstimulated cells | 375 | 667 |
Stimulated cells | 4660 | 2830 |
Note: Sample collection and handling procedures have a significant impact on measured RANTES levels. Refer to the Sample Collection and Storage section for the recommended sample collection procedures.
Product Summary
Precision
Cell Culture Supernates, Serum, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 91.9 | 573 | 1120 | 92 | 511 | 1105 |
Standard Deviation | 2.3 | 10 | 32.4 | 6.2 | 32.7 | 70.7 |
CV% | 2.5 | 1.7 | 2.9 | 6.7 | 6.4 | 6.4 |
EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision |
---|---|
Sample | 1 |
n | 20 |
Mean (pg/mL) | 108 |
Standard Deviation | 3.9 |
CV% | 3.6 |
Recovery
The recovery of RANTES spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 101 | 95-110 |
Platelet-poor Citrate Plasma (n=4) | 99 | 93-105 |
Platelet-poor EDTA Plasma (n=4) | 98 | 93-103 |
Platelet-poor Heparin Plasma (n=4) | 99 | 93-105 |
Serum (n=5) | 101 | 94-107 |
Urine (n=10) | 102 | 88-114 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CCL5/RANTES
RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), also known as CCL5, is a member of the "CC" subfamily of chemokines. It plays a primary role in the inflammatory immune response via its ability to chemoattract leukocytes and modulate their function. The cDNA for RANTES was initially discovered by subtractive hybridization as a T cell specific sequence (1, 2). Human RANTES cDNA encodes a highly basic 91 amino acid (aa) residue precursor polypeptide with a 23 aa hydrophobic signal peptide that is cleaved to generate the 68 aa mature protein (1, 2). Human RANTES exhibits approximately 85% homology with mouse RANTES at the deduced aa level (3, 4).
Citations for Human CCL5/RANTES Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants
Authors: Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A;
Microorganisms
Species: Human
Sample Types: Cell Culture Supernates
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NR1D1 stimulates antitumor immune responses in breast cancer by activating cGAS-STING signaling
Authors: Ka, NL;Park, MK;Kim, SS;Jeon, Y;Hwang, S;Kim, SM;Lim, GY;Lee, H;Lee, MO;
Cancer research
Species: Human
Sample Types: Cell Culture Supernates
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Effect of stimulator of interferon genes (STING) signaling on radiation-induced chemokine expression in human osteosarcoma cells
Authors: SS Withers, CE Moeller, CN Quick, CC Liu, SM Baham, JS Looper, R Subramania, KG Kousoulas
PLoS ONE, 2023-04-20;18(4):e0284645.
Species: Human
Sample Types: Cell Culture Supernates
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Effect of stimulator of interferon genes (STING) signaling on radiation-induced chemokine expression in human osteosarcoma cells
Authors: SS Withers, CE Moeller, CN Quick, CC Liu, SM Baham, JS Looper, R Subramania, KG Kousoulas
PLoS ONE, 2023;18(4):e0284645.
Species: Human
Sample Types: Cell Culture Supernates
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Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis
Authors: RO Ali, GM Quinn, R Umarova, JA Haddad, GY Zhang, EC Townsend, L Scheuing, KL Hill, M Gewirtz, S Rampertaap, SD Rosenzweig, AT Remaley, JM Han, V Periwal, H Cai, PJ Walter, C Koh, EB Levy, DE Kleiner, O Etzion, T Heller
Nature Microbiology, 2022-12-15;0(0):.
Species: Human
Sample Types: Serum
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CCL5 Levels Predict Stroke Volume Growth in Acute Ischemic Stroke and Significantly Diminish in Hemorrhagic Stroke Patients
Authors: FJ Julián-Vil, M Serrano-Po, E Ramalle-Gó, A Martínez, L Ochoa-Call
International Journal of Molecular Sciences, 2022-09-01;23(17):.
Species: Human
Sample Types: Serum
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Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes
Authors: J Baran, ? Niewiara, J Podolec, M Siedli?ski, E Józefczuk, A Bernacik, R Badacz, T Przew?ocki, P Pieni??ek, K ?mudka, J Legutko, A Kab?ak-Zie
Oncogene, 2022-06-17;9(6):.
Species: Human
Sample Types: Serum
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KIF4A promotes tumor progression of bladder cancer via CXCL5 dependent myeloid-derived suppressor cells recruitment
Authors: N Lin, L Chen, Y Zhang, Y Yang, L Zhang, L Chen, P Zhang, H Su, M Yin
Scientific Reports, 2022-04-10;12(1):6015.
Species: Human
Sample Types: Cell Culture Supernates
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IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer
Authors: NL Ka, GY Lim, S Hwang, SS Kim, MO Lee
Cell Reports, 2021-12-21;37(12):110138.
Species: Human
Sample Types: Cell Culture Supernates
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Metabolic modulation by CDK4/6 inhibitor promotes chemokine-mediated recruitment of T�cells into mammary tumors
Authors: RV Uzhachenko, V Bharti, Z Ouyang, A Blevins, S Mont, N Saleh, HA Lawrence, C Shen, SC Chen, GD Ayers, DG DeNardo, C Arteaga, A Richmond, AE Vilgelm
Cell Reports, 2021-04-06;35(1):108944.
Species: Human
Sample Types: Cell Culture Supernates
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NF&kapppaB-Activated COX2/PGE2/EP4 Axis Controls the Magnitude and Selectivity of BCG-Induced Inflammation in Human Bladder Cancer Tissues
Authors: OM Ibrahim, PH Basse, W Jiang, K Guru, G Chatta, P Kalinski
Cancers, 2021-03-16;13(6):.
Species: Human
Sample Types: Cell Culture Supernates
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In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
International Journal of Molecular Sciences, 2021-03-16;22(6):.
Species: Human
Sample Types: Plasma
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Tissue-specific endothelial cell heterogeneity contributes to unequal inflammatory responses
Authors: H Gunawardan, T Romero, N Yao, S Heidt, A Mulder, DA Elashoff, NM Valenzuela
Scientific Reports, 2021-01-21;11(1):1949.
Species: Human
Sample Types: Cell Culture Supernates
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Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH
Authors: J Dong, S Viswanatha, E Adami, BK Singh, SP Chothani, B Ng, WW Lim, J Zhou, M Tripathi, NSJ Ko, SG Shekeran, J Tan, SY Lim, M Wang, PM Lio, PM Yen, S Schafer, SA Cook, AA Widjaja
Nature Communications, 2021-01-04;12(1):66.
Species: Human
Sample Types: Cell Culture Supernates
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Loss of FOXF1 expression promotes human lung-resident mesenchymal stromal cell migration via ATX/LPA/LPA1 signaling axis
Authors: P Cao, NM Walker, RR Braeuer, S Mazzoni-Pu, Y Aoki, K Misumi, DS Wheeler, R Vittal, VN Lama
Scientific Reports, 2020-12-04;10(1):21231.
Species: Human
Sample Types: Cell Culture Supernates
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Proteomics of extracellular vesicles produced by Granulicatella adiacens, which causes infective endocarditis
Authors: SA Alkandari, RG Bhardwaj, A Ellepola, M Karched
PLoS ONE, 2020-11-20;15(11):e0227657.
Species: Human
Sample Types: Cell Culture Supernates
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Preconditioning mesenchymal stromal cells with flagellin enhances the anti?inflammatory ability of their secretome against lipopolysaccharide?induced acute lung injury
Authors: R Li, Y Li, X Dong
Molecular Medicine Reports, 2020-07-28;0(0):.
Species: Human
Sample Types: BALF
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Heatr9 is an infection responsive gene that affects cytokine production in alveolar epithelial cells
Authors: CJ Stairiker, M van Meurs, LG Leon, AA Brouwers-H, L Rijsbergen, YM Mueller, PD Katsikis
PLoS ONE, 2020-07-17;15(7):e0236195.
Species: Human
Sample Types: Cell Culture Supernates
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DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer
Authors: AA de Cubas, W Dunker, A Zaninovich, RA Hongo, A Bhatia, A Panda, KE Beckermann, G Bhanot, S Ganesan, J Karijolich, WK Rathmell
JCI Insight, 2020-06-04;5(11):.
Species: Human
Sample Types: Cell Culture Supernates
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Pharmacological inhibition of P2RX7 ameliorates liver injury by reducing inflammation and fibrosis
Authors: B Baeza-Raja, A Goodyear, X Liu, K Lam, L Yamamoto, Y Li, GS Dodson, T Takeuchi, T Kisseleva, DA Brenner, K Dabbagh
PLoS ONE, 2020-06-03;15(6):e0234038.
Species: Human
Sample Types: Cell Culture Supernates
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Diminished hepatic IFN response following HCV clearance triggers HBV reactivation in coinfection
Authors: X Cheng, T Uchida, Y Xia, R Umarova, CJ Liu, PJ Chen, A Gaggar, V Suri, MM Mücke, J Vermehren, S Zeuzem, Y Teraoka, M Osawa, H Aikata, K Tsuji, N Mori, S Hige, Y Karino, M Imamura, K Chayama, TJ Liang
J. Clin. Invest., 2020-06-01;0(0):.
Species: Mouse
Sample Types: Serum
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Plasma RANTES level is correlated with cardio-cerebral atherosclerosis burden in patients with ischemic cerebrovascular disease
Authors: Q Kong, X Ma, JX Lyu, C Wang, XY Du, YQ Guan
Chronic Dis Transl Med, 2020-03-10;6(1):46-54.
Species: Human
Sample Types: Plasma
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Defining the landscape of ATP-competitive inhibitor resistance residues in protein kinases
Authors: NS Persky, D Hernandez, M Do Carmo, L Brenan, O Cohen, S Kitajima, U Nayar, A Walker, S Pantel, Y Lee, J Cordova, M Sathappa, C Zhu, TK Hayes, P Ram, P Pancholi, TS Mikkelsen, DA Barbie, X Yang, R Haq, F Piccioni, DE Root, CM Johannesse
Nat. Struct. Mol. Biol., 2020-01-10;27(1):92-104.
Species: Human
Sample Types: Cell Culture Supernates
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Retinoblastoma inactivation induces a protumoral microenvironment via enhanced CCL2 secretion
Authors: F Li, S Kitajima, S Kohno, A Yoshida, S Tange, S Sasaki, N Okada, Y Nishimoto, H Muranaka, N Nagatani, M Suzuki, S Masuda, TC Thai, T Nishiuchi, T Tanaka, DA Barbie, N Mukaida, C Takahashi
Cancer Res., 2019-06-12;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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VSIG-3 as a ligand of VISTA inhibits human T-cell function.
Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
Immunology, 2018-10-10;156(1):74-85.
Species: Human
Sample Types: Cell Culture Supernates
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Suppression of STING associated with LKB1 loss in KRAS-driven lung cancer
Authors: S Kitajima, E Ivanova, S Guo, R Yoshida, M Campisi, SK Sundararam, S Tange, Y Mitsuishi, TC Thai, S Masuda, BP Piel, LM Sholl, PT Kirschmeie, CP Paweletz, H Watanabe, M Yajima, DA Barbie
Cancer Discov, 2018-10-08;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Induction of chemokine (C-C motif) ligand 5 by Epstein-Barr virus infection enhances tumor angiogenesis in nasopharyngeal carcinoma
Authors: W Ma, L Feng, S Zhang, H Zhang, X Zhang, X Qi, Y Zhang, Q Feng, T Xiang, YX Zeng
Cancer Sci., 2018-04-28;109(5):1710-1722.
Species: Human
Sample Types: Cell Culture Supernates
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C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
Authors: S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami
Cancer Sci., 2018-02-14;109(3):724-731.
Species: Human
Sample Types: Cell Culture Supernates
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Rapid Generation of Multiple Loci-Engineered Marker-free Poxvirus and Characterization of a Clinical-Grade Oncolytic Vaccinia Virus
Authors: ZS Guo, Z Liu, M Sathaiah, J Wang, R Ravindrana, E Kim, S Huang, TW Kenniston, JC Bell, HJ Zeh, LH Butterfiel, A Gambotto, DL Bartlett
Mol Ther Methods Clin Dev, 2017-09-30;7(0):112-122.
Species: Human
Sample Types: Cell Culture Supernates
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Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women
Authors: A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin
Oncotarget, 2017-09-15;8(54):92134-92142.
Species: Human
Sample Types: Cell Culture Supernates
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Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
J Transl Med, 2017-05-31;15(1):121.
Species: Human
Sample Types: Plasma
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Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
Oncol Lett, 2017-04-21;13(6):4995-5001.
Species: Human
Sample Types: Cell Culture Supernates, Serum
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TWEAK/Fn14 promotes pro-inflammatory cytokine secretion in hepatic stellate cells via NF-?B/STAT3 pathways
Authors: A Wang, F Zhang, H Xu, M Xu, Y Cao, C Wang, Y Xu, M Su, M Zhang, Y Zhuge
Mol. Immunol., 2017-04-12;87(0):67-75.
Species: Human
Sample Types: Cell Culture Supernates
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CD8+ T cells promote proliferation of benign prostatic hyperplasia epithelial cells under low androgen level via modulation of CCL5/STAT5/CCND1 signaling pathway
Authors: Y Yang, S Hu, J Liu, Y Cui, Y Fan, T Lv, L Liu, J Li, Q He, W Han, W Yu, Y Sun, J Jin
Sci Rep, 2017-02-20;7(0):42893.
Species: Human
Sample Types: Cell Culture Supernates
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Tumor necrosis factor alpha inhibitors have no effect on a human T-lymphotropic virus type-I (HTLV-I)-infected cell line from patients with HTLV-I-associated myelopathy
Authors: S Fukui, H Nakamura, Y Takahashi, N Iwamoto, H Hasegawa, K Yanagihara, T Nakamura, A Okayama, A Kawakami
BMC Immunol, 2017-02-03;18(1):7.
Species: Human
Sample Types: Cell Culture Supernates
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-2518 A/G MCP-1 but not -403 G/A RANTES gene polymorphism is associated with enhanced risk of basal cell carcinoma
Authors: Roman Nowicki
Postepy Dermatol Alergol, 2016-10-21;33(5):381-385.
Species: Human
Sample Types: Serum
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Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions
Cytokine, 2016-05-18;84(0):17-24.
Species: Human
Sample Types: Plasma
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Loss of intraepidermal nerve fiber density during SIV peripheral neuropathy is mediated by monocyte activation and elevated monocyte chemotactic proteins.
Authors: Lakritz J, Robinson J, Polydefkis M, Miller A, Burdo T
J Neuroinflammation, 2015-12-18;12(0):237.
Species: Primate
Sample Types: Plasma
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Hypoxic Preconditioning Increases Survival and Pro-Angiogenic Capacity of Human Cord Blood Mesenchymal Stromal Cells In Vitro.
Authors: Bader A, Klose K, Bieback K, Korinth D, Schneider M, Seifert M, Choi Y, Kurtz A, Falk V, Stamm C
PLoS ONE, 2015-09-18;10(9):e0138477.
Species: Human
Sample Types: Cell Culture Supernates
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Serum levels of chemokines CCL4 and CCL5 in cirrhotic patients indicate the presence of hepatocellular carcinoma.
Authors: Sadeghi M, Lahdou I, Oweira H, Daniel V, Terness P, Schmidt J, Weiss K, Longerich T, Schemmer P, Opelz G, Mehrabi A
Br J Cancer, 2015-08-13;113(5):756-62.
Species: Human
Sample Types: Serum
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The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
J Immunol, 2015-07-24;195(5):2429-41.
Species: Human
Sample Types: Cell Culture Supernates
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Nuclear NF-kappaB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.
Authors: Yi B, Hu X, Zhang H, Huang J, Liu J, Hu J, Li W, Huang L
PLoS ONE, 2014-06-17;9(6):e99633.
Species: Human
Sample Types: Urine
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IL-10 up-regulates CCL5 expression in vascular smooth muscle cells from spontaneously hypertensive rats.
Authors: Kim H, Kim H
Cytokine, 2014-03-19;68(1):40-9.
Species: Rat
Sample Types: Cell Culture Supernates
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Chlorogenic acid inhibits human platelet activation and thrombus formation.
Authors: Fuentes E, Caballero J, Alarcon M, Rojas A, Palomo I
PLoS ONE, 2014-03-05;9(3):e90699.
Species: Human
Sample Types: Cell Culture Supernates
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Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
Br J Haematol, 2013-10-28;164(2):286-95.
Species: Human
Sample Types: Serum
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Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost., 2012-08-01;10(8):1616-23.
Species: Human
Sample Types: Serum
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Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue Fever.
Authors: de-Oliveira-Pinto LM, Marinho CF, Povoa TF, de Azeredo EL, de Souza LA, Barbosa LD, Motta-Castro AR, Alves AM, Avila CA, de Souza LJ, da Cunha RV, Damasco PV, Paes MV, Kubelka CF
PLoS ONE, 2012-07-16;7(7):e38527.
Species: Human
Sample Types: Plasma
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Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.
Authors: Tavoosidana G, Ronquist G, Darmanis S, Yan J, Carlsson L, Wu D, Conze T, Ek P, Semjonow A, Eltze E, Larsson A, Landegren UD, Kamali-Moghaddam M
Proc. Natl. Acad. Sci. U.S.A., 2011-05-09;108(21):8809-14.
Species: Human
Sample Types: Platelet Poor Plasma
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Persistently raised plasma levels of platelet-derived inflammatory mediators in HIV-infected patients during highly active anti-retroviral therapy.
Authors: Landro L, Ueland T, Otterdal K, Froland SS, Aukrust P
J. Thromb. Haemost., 2011-05-01;9(5):1075-7.
Species: Human
Sample Types: Platelet Poor Plasma
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Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
Authors: Tso GH, Law HK, Tu W
Stem Cells, 2010-05-01;28(5):939-54.
Species: Human
Sample Types: Cell Culture Supernates
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Human CD4- 8- T cells are a distinctive immunoregulatory subset.
Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
FASEB J, 2010-02-12;24(7):2558-66.
Species: Human
Sample Types: Cell Culture Supernates
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A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets.
Authors: Su Y, Amiri KI, Horton LW
Clin. Cancer Res., 2009-12-22;16(1):348-57.
Species: Human
Sample Types: Serum
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Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Authors: Renaud SJ, Sullivan R, Graham CH
Placenta, 2009-02-08;30(4):313-9.
Species: Human
Sample Types: Cell Culture Supernates
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HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
J. Exp. Med., 2009-01-12;206(1):169-81.
Species: Human
Sample Types: Cell Culture Supernates
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Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
Cancer Res., 2008-12-01;68(23):9996-10003.
Species: Human
Sample Types: Serum
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Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
Authors: Lee YR, Kweon SH, Kwon KB, Park JW, Yoon TR, Park BH
Biochem. Biophys. Res. Commun., 2008-11-11;378(1):90-4.
Species: Human
Sample Types: Cell Culture Supernates
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NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
J. Immunol., 2008-11-01;181(9):5857-64.
Species: Human
Sample Types: Cell Culture Supernates
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RANTES in exhaled breath condensate of stable and unstable asthma patients.
Authors: Zietkowski Z, Tomasiak MM, Skiepko R, Bodzenta-Lukaszyk A
Respir Med, 2008-07-07;102(8):1198-202.
Species: Human
Sample Types: Exhaled Breath Condensate (EBC)
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Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
J. Neuroimmunol., 2008-06-09;199(1):35-45.
Species: Human
Sample Types: Cell Culture Supernates
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In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
J. Interferon Cytokine Res., 2008-05-01;28(5):297-310.
Species: Human
Sample Types: Cell Culture Supernates
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Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
Life Sci., 2008-04-20;82(23):1203-9.
Species: Human
Sample Types: Cell Culture Supernates
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CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
Biochem. Pharmacol., 2008-04-18;76(1):79-90.
Species: Human
Sample Types: Cell Culture Supernates
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Differential chemokine expression in tubular cells in response to urinary proteins from patients with nephrotic syndrome.
Authors: Huang Z, Wen Q, Zhou SF, Yu XQ
Cytokine, 2008-03-24;42(2):222-33.
Species: Human
Sample Types: Urine
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Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
Int. J. Cancer, 2008-02-15;122(4):769-76.
Species: Human
Sample Types: Cell Culture Supernates
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Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
Blood, 2007-12-10;111(4):2339-46.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating interleukin-15 and RANTES chemokine in Parkinson's disease.
Authors: Rentzos M, Nikolaou C, Andreadou E, Paraskevas GP, Rombos A, Zoga M, Tsoutsou A, Boufidou F, Kapaki E, Vassilopoulos D
Acta Neurol. Scand., 2007-12-01;116(6):374-9.
Species: Human
Sample Types: Serum
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Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
J. Allergy Clin. Immunol., 2007-11-01;120(5):1118-25.
Species: Human
Sample Types: BALF
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Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis.
Authors: Barrera L, Mendoza F, Zuniga J, Estrada A, Zamora AC, Melendro EI, Ramirez R, Pardo A, Selman M
Am. J. Respir. Crit. Care Med., 2007-10-18;177(1):44-55.
Species: Human
Sample Types: BALF
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CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
Chest, 2007-10-09;133(1):26-33.
Species: Human
Sample Types: Saliva
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Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.
Authors: Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA
Nature, 2007-10-04;449(7162):557-63.
Species: Human
Sample Types: Cell Culture Supernates
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Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
Authors: Bolovan-Fritts CA, Spector SA
Blood, 2007-09-25;111(1):175-82.
Species: Human
Sample Types: Cell Culture Supernates
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Relationship between eosinophilia and levels of chemokines (CCL5 and CCL11) and IL-5 in bronchoalveolar lavage fluid of patients with mustard gas-induced pulmonary fibrosis.
Authors: Emad A, Emad Y
J. Clin. Immunol., 2007-07-10;27(6):605-12.
Species: Human
Sample Types: BALF
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Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
Authors: Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
J. Immunol., 2007-06-15;178(12):7678-85.
Species: Human
Sample Types: Cell Culture Supernates
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High T-cell response to human cytomegalovirus induces chemokine-mediated endothelial cell damage.
Authors: Bolovan-Fritts CA, Trout RN, Spector SA
Blood, 2007-05-22;110(6):1857-63.
Species: Human
Sample Types: Cell Culture Supernates
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An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
J. Exp. Med., 2006-11-20;203(12):2763-77.
Species: Human
Sample Types: Cell Culture Supernates
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Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
J. Am. Coll. Cardiol., 2006-09-27;48(8):1591-9.
Species: Human
Sample Types: Cell Culture Supernates
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Regulated upon activation, normal T cell expressed and secreted (RANTES) and monocyte chemotactic protein 1 in follicular fluid accumulate differentially in patients with and without endometriosis undergoing in vitro fertilization.
Authors: Schultze-Mosgau A, Agic A, Diedrich K, Hornung D
Fertil. Steril., 2006-09-25;86(6):1616-20.
Species: Human
Sample Types: Serum
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Interaction between the CCR5 chemokine receptors and microbial HSP70.
Authors: Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T
Eur. J. Immunol., 2006-09-01;36(9):2304-14.
Species: Human
Sample Types: Cell Culture Supernates
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RANTES and monocyte chemoattractant protein 1 as sensitive markers of disease activity in patients with juvenile rheumatoid arthritis: a six-year longitudinal study.
Authors: Yao TC, Kuo ML, See LC, Ou LS, Lee WI, Chan CK, Huang JL
Arthritis Rheum., 2006-08-01;54(8):2585-93.
Species: Human
Sample Types: Serum
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Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
Int. Immunol., 2006-06-01;18(8):1243-51.
Species: Human
Sample Types: Cell Culture Supernates
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Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
Authors: Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
J. Immunol., 2006-03-15;176(6):3788-95.
Species: Human
Sample Types: Cell Culture Supernates
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Increased ENA-78 in the follicular fluid of patients with endometriosis.
Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42.
Species: Human
Sample Types: Follicular Fluid
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Airway inflammation during stable and acutely exacerbated chronic obstructive pulmonary disease.
Authors: Fujimoto K, Yasuo M, Urushibata K, Hanaoka M, Koizumi T, Kubo K
Eur. Respir. J., 2005-04-01;25(4):640-6.
Species: Human
Sample Types: Sputum
-
Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
Microbes Infect., 2005-02-24;7(3):427-36.
Species: Human
Sample Types: Serum
-
CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
Transplantation, 2004-11-15;78(9):1341-50.
Species: Human
Sample Types: Urine
-
Correlations between IL-4, IL-12 levels and CCL2, CCL5 levels in serum and cerebrospinal fluid of multiple sclerosis patients.
Authors: Bartosik-Psujek H, Stelmasiak Z
J Neural Transm, 2004-10-12;112(6):797-803.
Species: Human
Sample Types: Serum
-
Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
J. Gen. Virol., 2004-09-01;85(0):2603-13.
Species: Human
Sample Types: Cell Culture Supernates
-
Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
Am. J. Respir. Crit. Care Med., 2004-06-10;170(5):508-15.
Species: Human
Sample Types: Cell Culture Supernates
-
Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus.
Authors: Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, Walker C, Bhardwaj N
J. Virol., 2004-06-01;78(12):6151-61.
Species: Primate - Pan troglodytes (Chimpanzee)
Sample Types: Cell Culture Supernates
-
Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
J. Exp. Med., 2004-05-03;199(9):1213-21.
Species: Human
Sample Types: Cell Culture Supernates
-
Impact of NNRTI compared to PI-based highly active antiretroviral therapy on CCR5 receptor expression, beta-chemokines and IL-16 secretion in HIV-1 infection.
Authors: Burton CT, »V6þÌ™+ìZ, Hardy GA, Sullivan AK, Nelson MR, Gazzard B, Gotch FM, Imami N
2002-11-01;130(2):286-92.
Species: Human
Sample Types: Plasma
-
Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
J. Immunol., 2002-10-15;169(8):4172-82.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Culture Supernates
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CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
Blood, 2002-10-01;100(7):2321-9.
Species: Human
Sample Types: Cell Culture Supernates
-
TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
Eur. J. Immunol., 2002-07-01;32(7):2037-45.
Species: Human
Sample Types: Cell Culture Supernates
-
Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites.
Authors: Milliken D, Scotton C, Raju S, Balkwill F, Wilson J
Clin. Cancer Res., 2002-04-01;8(4):1108-14.
Species: Human
Sample Types: Serum
-
Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Authors: Binninger-Schinzel D, 2019, Norley S, Adler HS, Oberg HH, Kurth R
2002-03-01;294(1):209-21.
Species: Human
Sample Types: Cell Culture Supernates
-
Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Authors: Sato E, Koyama S
Eur. Respir. J., 2000-11-01;16(5):951-8.
Species: Human
Sample Types: Cell Culture Supernates
-
Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production.
Authors: Gauduin MC, Glickman RL, Ahmad S, Yilma T, Johnson RP
Proc. Natl. Acad. Sci. U.S.A., 1999-11-23;96(24):14031-6.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Culture Supernates
-
Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques.
Authors: Lehner T, —îÊëÖ“§, Wang Y, r¹oŽ&A½ýx, Cranage M, Ïï§Ù´âoÑ‘, Bergmeier LA, Mitchell E, Tao L, Hall G, Dennis M, Cook N, Brookes R, Klavinskis L, Jones I, Doyle C, Ward R
1996-07-01;2(7):767-75.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Culture Supernates
FAQs
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Can plasma be used as a sample in Human CCL5/RANTES Quantikine® ELISA kit (Catalog # DRN00B) if platelet-poor plasma is not available?
Platelet-poor plasma is the only type of plasma sample validated for Human CCL5/RANTES Quantikine® ELISA kit. CCL5/RANTES is present in platelet granules and is released upon platelet activation.
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Average Rating: 4.2 (Based on 5 Reviews)
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Measured urine at 2-fold dilution and plasma at 4-fold dilution from healthy controls & patients with disease. All urine samples were below the standard curve. Plasma samples were mostly above the standard curve and would need further dilution.
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