Glycinergic Neuronal Markers

This interactive graphic lists some of the most commonly used glycinergic neuronal markers including the Glycine Transporter 2 (GlyT2) and the Vesicular Inhibitory Amino Acid Transporter (VIAAT). Clicking on a marker will take you to the R&D Systems selection of antibodies directed against that molecule. Clicking on a marker that is designated by (novusbio.com) will take you to the Novus Biologicals’ website and their antibody offerings for that protein. Additionally, use the buttons below to see markers associated with different neuronal subtypes.

Cholinergic Dopaminergic GABAergic Glutamatergic Glycinergic Serotonergic

Glycinergic

GlyT2/SLC6A5

VIAAT/SLC32A1

Overview

Glycinergic neurons utilize the amino acid glycine as a neurotransmitter. Glycine is the main inhibitory neurotransmitter in the brainstem and spinal cord. Binding of glycine to its receptors opens Cl- channels, allowing for influx of Cl- and hyperpolarization. However, glycine has also been shown to have excitatory effects. It acts as a glutamate co-agonist and is required for NMDA receptor activity.

Data Examples

Immunohistochemistry-VIAAT/SLC32A1 in Human Brain

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VIAAT in Human Brain. The Vesicular Inhibitory Amino Acid Transporter (VIAAT), also called SLC32A1, was detected in immersion-fixed paraffin-embedded sections of human brain using a Sheep Anti-Human VIAAT/SLC32A1 Antigen Affinity-Purified Polyclonal Antibody (R&D Systems, Catalog # AF6847). Before incubation with the primary antibody, the tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (R&D Systems, Catalog # CTS013). The tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (R&D Systems, Catalog # CTS019; brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes.