GABAergic Neuronal Markers

Overview

GABAergic neurons produce the neurotransmitter gamma-Aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. It is synthesized from glutamate by two isoforms of Glutamate Decarboxylase (GAD), GAD65 and GAD67. About twenty percent of neurons in the brain use GABA as a neurotransmitter, and they are widely distributed throughout the brain. GABA suppresses neural activity by inducing either Cl^- influx or K⁺ efflux, thereby hyperpolarizing neurons. GABA is involved in motor control, vision, and other cortical functions. It has also been shown to regulate anxiety. GABA dysfunction is associated with epilepsy, stiff-person syndrome, and schizophrenia.

Data Examples

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GAD67 in Human Spinal Cord. GAD67, also called GAD1, was detected in immersion-fixed paraffin-embedded sections of human spinal cord using a Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-Purified Polyclonal Antibody (R&D Systems, Catalog # AF2086). The tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems, Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

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GAD65 in Human Dorsal Root Ganglia. GAD65, also called GAD2, was detected in immersion-fixed paraffin-embedded sections of human dorsal root ganglia using a Goat Anti-Human GAD2/GAD65 Antigen Affinity-Purified Polyclonal Antibody (R&D Systems, Catalog # AF2247). The tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems, Catalog # CTS008; brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots.