Human SorCS1 Antibody

Catalog #: AF3457
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3457
AF3457-SP

All SorCS1 Antibodies

Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SorCS1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SorCS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse SorCS1 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS2 and rhSorCS3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SorCS1
Ser111-Ser1099 (Ser231Gly)
Accession # Q8WY21.3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human SorCS1 (Catalog # 3457-SR)

Immunocytochemistry

5-15 µg/mL

Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorCS1

SorCS1 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. Three splicing variants (SorCS1a, b and c) differ only in their cytoplasmic domains (3). All variants are predominantly expressed in the central nervous system, but SorCS1 can also be identified in heart, kidney and pancreatic islets (2‑5). SorCS1a mediates endocytosis, and only ~10% of it is expressed on the cell surface. SorCS1b shows higher surface expression (~45%) and is much less involved in endocytosis. SorCS1c is intermediate. Human SorCS1a is synthesized as a 1159 amino acid (aa) preproform with a 33 aa signal sequence and a 77 aa propeptide. After proteolytic processing at a furin-type consensus sequence, the mature SorCS1a is a 1049 aa, 130 kDa protein with a 989 aa extracellular/lumenal domain (ECD). Within the ECD, human SorCS1 shares 93%, 94%, 93% and 98% aa identity with mouse, rat, bovine and canine SorCS1, respectively. It also shares 70% and 46% aa identity with the ECD of human SorCS3 and SorCS2, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain and binds the growth factor PDGF-BB (1, 2, 6). Expression in the hippocampus indicates that SorCS1 may modulate PDGF-BB activity in this location (6). SorCS1 has also been identified as a susceptibility gene for type 2 diabetes in overweight females (4). Consequently, it has been proposed to affect insulin secretion by modifying PDGF-mediated growth of the islet vasculature (7). The 80 kDa ECD may be constitutively or inducibly shed, mainly via the metalloproteinase TACE/ADAM17 (6). The shed soluble form also binds PDGF. The cellular portion appears to undergo regulated intramembrane proteolysis (8).

References

Hampe, W. et al. (2001) Hum. Genet. 108:529.
Hermey, G. et al. (1999) Biochem. Biophys. Res. Commun. 266:347.
Hermey, G. et al. (2003) J. Biol. Chem. 278:7390.
Clee, S.M. et al. (2006) Nat. Genet. 38:688.
Hermey, G. et al. (2001) Neurosci. Lett. 313:83.
Hermey, G. et al. (2006) Biochem. J. 395:285.
Goodarzi, M.O. et al. (2007) Diabetes Apr 10 [Epub ahead of print].
Nyborg, A.C. et al. (2006) Mol. Neurodegen. 1:3.

Long Name

Sortilin-related VPS10 Domain Containing Receptor 1

Entrez Gene IDs

114815 (Human); 58178 (Mouse); 309533 (Rat)

Alternate Names

FLJ41758; FLJ43475; FLJ44957; hSorCS; SORCS receptor 1; SORCS; SorCS1; sortilin-related VPS10 domain containing receptor 1; VPS10 domain receptor protein SORCS 1; VPS10 domain receptor SorCS; VPS10 domain-containing receptor SorCS1

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human SorCS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Prions amplify through degradation of the VPS10P sorting receptor sortilin
Authors: K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi
PLoS Pathog., 2017-06-30;13(6):e1006470.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
SORCS1 alters amyloid precursor protein processing and variants may increase Alzheimer's disease risk.
Authors: Reitz C, Tokuhiro S, Clark LN, Conrad C, Vonsattel JP, Hazrati LN, Palotas A, Lantigua R, Medrano M, Z Jimenez-Velazquez I, Vardarajan B, Simkin I, Haines JL, Pericak-Vance MA, Farrer LA, Lee JH, Rogaeva E, George-Hyslop PS, Mayeux R
Ann. Neurol., 2011-01-01;69(1):47-64.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Targeted manipulation of the sortilin-progranulin axis rescues progranulin haploinsufficiency.
Authors: Lee WC, Almeida S, Prudencio M et al.
Hum Mol Genet.