VisUCyte™ HRP Polymer - Cleaner, Faster IHC Results
VisUCyte™ HRP Polymer is a Biotin-free detection reagent that overcomes problems associated with Avidin-Biotin detection chemistry, such as the extra quenching steps required to inhibit endogenous Biotin and Avidin staining. R&D Systems offers secondary antibodies that are conjugated to the VisUCyte™ HRP Polymer and complete staining kits that contain VisUCyte™ HRP Polymer-conjugated secondary antibodies, the DAB chromogen, buffers, and blocking reagent.
VisUCyte HRP Polymer outperforms conventional Avidin-Biotin-HRP detection by providing the following advantages:
- High-sensitivity detection: Detect low-abundant protein targets, saving costs with reduced primary antibody usage.
- Lack of non-specific staining: Specific signal stays clear without interference from irrelevant staining profiles.
- Avidin and biotin-free: Skip the need to quench endogenous biotin, preventing non-specific tissue staining.
- Swift protocol: Our time-efficient staining procedure accelerates scientific data generation.
- Ready-to-use HRP polymer conjugated secondary antibodies: No hassle with staining optimization.
Compare IHC Staining Using the VisUCyte™ HRP Polymer versus Avidin-Biotin
GIT1 Detection in Human Brain Using the VisUCyte™ HRP Polymer. GIT1 was detected in immersion-fixed paraffin-embedded tissue sections of human brain (cerebral cortex) using a Rabbit Anti-Human GIT1 Antigen Affinity-Purified Polyclonal Antibody (Catalog #AF8508), followed by a Goat Anti-Rabbit IgG VisUCyte™ HRP Polymer Secondary Antibody (Catalog #VC003). The tissue was stained with DAB (brown) and counterstained with hematoxylin (blue).
GIT1 Detection in Human Brain Using the ABC Method. GIT1 was detected in immersion-fixed paraffin-embedded tissue sections of human brain (cerebral cortex) using a Rabbit Anti-Human GIT1 Antigen Affinity-Purified Polyclonal Antibody (Catalog #AF8508), followed by a Biotin-conjugated anti-rabbit secondary antibody. The tissue was stained using the ABC Method (brown) and counterstained with hematoxylin (blue).
Secondary Antibody Control for the VisUCyte™ HRP Polymer. Immersion-fixed paraffin-embedded tissue sections of human brain (cerebral cortex) were incubated with a Goat Anti-Rabbit IgG VisUCyte™ HRP Polymer Secondary Antibody (Catalog # VC003), followed by DAB. The tissue was counterstained with hematoxylin (blue). The Rabbit Anti-Human GIT1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF8508) was omitted from the staining procedure.
View Our Protocol for Using the VisUCyte™ HRP Polymer
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