Human beta-NGF Antibody

Name: Human beta-NGF Antibody
Brand: R&D Systems
SKU: AF-256-NA
Rating: 4 (1 reviews)

Catalog #: AF-256-NA
6 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-256-NA
AF-256-SP

Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody.

2 Images

All beta-NGF Antibodies

Product Details

Citations (6)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta-NGF Antibody Summary

Specificity

Detects human beta -NGF in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant mouse
beta -NGF and recombinant rat beta -NGF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta -NGF
Ser122-Ala241
Accession # CAA36832

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human beta -NGF (Catalog # 256-GF)

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.02-0.08 µg/mL in the presence of 5 ng/mL Recombinant Human beta ‑NGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. Recombinant Human beta -NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta -NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-256-NA). The ND₅₀ is typically 0.02-0.08 µg/mL.

Immunohistochemistry

View Larger

beta ‑NGF in Human Brain. beta -NGF was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-256-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to axons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name

beta Nerve Growth Factor

Entrez Gene IDs

4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)

Alternate Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human beta-NGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants
Authors: AM Alhilou, A Shimada, CI Svensson, P Svensson, M Ernberg, BE Cairns, N Christidis
Scientific Reports, 2021-07-06;11(1):13873.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons
Authors: P Pagella, J Catón, CT Meisel, TA Mitsiadis
Cells, 2020-03-06;9(3):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
Sci Rep, 2017-03-09;7(0):44266.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
Mol. Ther., 2010-07-27;18(12):2164-72.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Nerve growth factor and artemin are paracrine mediators of pancreatic neuropathy in pancreatic adenocarcinoma.
Authors: Ceyhan GO, Schafer KH, Kerscher AG, Rauch U, Demir IE, Kadihasanoglu M, Bohm C, Muller MW, Buchler MW, Giese NA, Erkan M, Friess H
Ann. Surg., 2010-05-01;251(5):923-31.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Immunodepletion
Atropine-enhanced, antigen challenge-induced airway hyperreactivity in guinea pigs is mediated by eosinophils and nerve growth factor.
Authors: Verbout NG, Jacoby DB, Gleich GJ, Fryer AD
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-05-15;297(2):L228-37.
Species: Guinea Pig
Sample Types: In Vivo
Applications: Neutralization