LI-COR Data Examples for Human Adipokine Array Kit

Data Examples

Figure 1. The Human Adipokine Array detects multiple analytes in cell culture supernates. Cells were either untreated or treated as indicated below. Each array was incubated with 500 µL of cell culture supernate. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. Peripheral blood mononuclear cells were either untreated or treated with 10 µg/mL of PHA for 24 h. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 h.

Figure 2. The Human Adipokine Array detects multiple analytes in cell lysates. Cells were either untreated or treated as indicated below. Each array was incubated with 200 µg of cell lysate. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. THP-1 human acute monocytic leukemia cells were either untreated or treated with 1 µg/mL of recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 h, followed by 1 µg/mL of LPS for 16 h. MG63 human osteosarcoma cells were untreated or treated with 1 ng/mL of recombinant human IL-1 beta (R&D Systems, Catalog # 201-LB) for 48 h.

Integrated Intensity Pre-adipocytes 3 Week Old Adipocytes Adipose Tissue 1 Pentraxin-3/TSG-14 29635 20454 840 2 IGFBP-3 45746 25432 924 3 IGFBP-4 13638 16169 1196 4 IGFBP-7/IGFBP-rp1 34614 44713 2232 5 MIF 9223 2804 23910 6 IL-8/CXCL8 49400 1406 494 7 Adiponectin/ACRP30 524 40844 10324 8 Leptin 634 13814 435 9 Complement Factor D 2067 21045 14931 10 Cathepsin D 6395 16089 33705 11 Serpin E1/PAI-1 16774 23591 702 12 ICAM-I 1065 554 10883 13 C-Reactive Protein/CRP 669 550 8819 14 Lipocalin-2/NGAL 749 554 10658 15 DPPIV/CD26 1459 805 6118 16 FGF basic 1617 1234 2953 17 Nidogen-1/Entactin 5908 47401 7067 18 Fibrinogen 1885 1113 11631

Figure 3. The Human Adipoline Array detects multiple analytes in cultured human adipocytes and adipose tissue lysate. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. Undifferentiated human pre-adipocytes; 500 µL of cell culture supernate per array. 3 week old human adipocytes; 500 µL of cell culture supernate per array. Adipose tissue; 200 µg tissue lysate per array.

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