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Human BDNF Antibody

Catalog #: MAB248
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB248-SP
MAB248-100
MAB248-500
BDNF in Human Spinal Cord.
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Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BDNF Antibody Summary

Species Reactivity
Human
Specificity
Detects human BDNF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross-reactivity with recombinant human (rh) beta -NGF and rr beta -NGF.
Source
Monoclonal Mouse IgG1 Clone # 35928
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
Arg128-Arg247
Accession # P23560
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BDNF (Catalog # 248-BD)
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry BDNF antibody in Human Spinal Cord by Immunohistochemistry (IHC-P). View Larger

BDNF in Human Spinal Cord. BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Human BDNF Monoclonal Antibody (Catalog # MAB248) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

References
  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Brain-derived Neurotrophic Factor
Entrez Gene IDs
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

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Citations for Human BDNF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Ultrasound Used for Diagnostic Imaging Facilitates Dendritic Branching of Developing Neurons in the Mouse Cortex
    Authors: T Papp, Z Ferenczi, B Szilagyi, M Petro, A Varga, E Kókai, E Berenyi, G Olah, G Halmos, P Szucs, Z Meszar
    Frontiers in Neuroscience, 2022-03-17;16(0):803356.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Requirements of Postnatal proBDNF in the Hippocampus for Spatial Memory Consolidation and Neural Function
    Authors: Wei Sun, Hong Cheng, Yang Yang, Dongxin Tang, Xiaolian Li, Lei An
    Frontiers in Cell and Developmental Biology
  3. Expression of degenerative markers in intervertebral discs of young and elderly asymptomatic individuals
    Authors: Josemberg S. Baptista, Vincent C. Traynelis, Edson A. Liberti, Ricardo B. V. Fontes
    PLOS ONE
  4. Leucine-rich repeat kinase-2 (LRRK2) modulates paraquat-induced inflammatory sickness and stress phenotype
    Authors: C Rudyk, Z Dwyer, S Hayley
    J Neuroinflammation, 2019-06-07;16(1):120.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. CD44v3+/CD24- cells possess cancer stem cell-like properties in human oral squamous cell carcinoma.
    Authors: Todoroki K, Ogasawara S, Akiba J, Nakayama M, Naito Y, Seki N, Kusukawa J, Yano H
    Int J Oncol, 2015-11-20;48(1):99-109.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Brain-derived neurotrophic factor and neurotrophin-4/5 are expressed in breast cancer and can be targeted to inhibit tumor cell survival.
    Authors: Vanhecke E, Adriaenssens E, Verbeke S, Meignan S, Germain E, Berteaux N, Nurcombe V, Le Bourhis X, Hondermarck H
    Clin. Cancer Res., 2011-02-24;17(7):1741-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Decreased BDNF levels are a major contributor to the embryonic phenotype of huntingtin knockdown zebrafish.
    Authors: Diekmann H, Anichtchik O, Fleming A, Futter M, Goldsmith P, Roach A, Rubinsztein DC
    J. Neurosci., 2009-02-04;29(5):1343-9.
    Species: Zebrafish
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
    Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
    Cancer Res., 2008-12-01;68(23):9996-10003.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Brain-derived neurotrophic factor (BDNF) and TrkB in the piglet brainstem after post-natal nicotine and intermittent hypercapnic hypoxia.
    Authors: Tang S, Machaalani R, Waters KA
    Brain Res., 2008-07-19;1232(0):195-205.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
    Authors: Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C
    J. Neuroimmunol., 2008-06-13;197(2):128-39.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  11. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
    Authors: Samah B, Porcheray F, Gras G
    Clin. Exp. Immunol., 2008-01-08;151(3):476-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Brain-derived neurotrophic factor mRNA and protein in the piglet brainstem and effects of Intermittent Hypercapnic Hypoxia.
    Authors: Peiris TS, Machaalani R, Waters KA
    Brain Res., 2004-12-10;1029(1):11-23.
    Species: Porcine
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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