There is great interest in identifying downstream T cell responses following immune checkpoint blockade to direct more effective treatments and better-informed therapeutic decisions. R&D Systems and Tocris offer a wide selection of reagents, kits, and assays that will allow you to explore, modulate, and validate T cell responses downstream of immune checkpoint blockade.
Immune Checkpoint Blockade Homepage
Explore
Proteome Profiler™ Antibody Arrays
Analyze the expression levels or phosphorylation status of up to 111 proteins simultaneously. Imagine the time you’ll save investigating how your checkpoint blockade affects downstream responses!
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Cytokine Profile of TNF-alpha-treated Balb/3T3 Cells. Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of Recombinant Mouse TNF-alpha (Catalog # 410-MT) for 24 hours. Cell supernates were analyzed using the Mouse XL Cytokine Array Kit (Catalog # ARY028). View All Antibody Arrays |
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Signal Transduction Profile of IGF-I-treated MCF-7 Cells. MCF-7 human breast cancer cells were either left untreated or treated with 100 ng/mL of Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. Cell lysates were analyzed using the Human Phospho-Kinase Array Kit (Catalog # ARY003B). |
Luminex® Screening Assays
Utilize our Luminex® Screening Assays to analyze the expression levels of up to 100 analytes simultaneously! Our Screening Assays are cost-effective, accurate, flexible, and require only a small sample volume (‹50 µL).
| Luminex Screening Assays |  |
Luminex Assay Ordering Tool |
Modulate
Tocris provides high performance research tools that activate and inhibit key downstream mediators of T-cell signaling, allowing you to modulate and investigate key proteins involved in immune checkpoint blockade. Tocris small molecules and peptides are manufactured to industry leading standards of purity and quality.
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| Primary Target | Cat. No. | Name | Description |
| Akt (Protein Kinase B) | 4635 | SC 79 | Akt activator |
| AP-1 | 2476 | SR 11302 | AP-1 inhibitor |
| Brutons Tyrosine Kinase (BTK) | 5012 | PCI 29732 | Potent BTK inhibitor |
| c-FMS (CSF1R) | 4481 | Ki 20227 | c-Fms inhibitor |
| ERK | 3706 | FR 180204 | Selective ERK inhibitor |
| G Proteins (Small) | 4266 | ML 141 | Selective inhibitor of Cdc42 Rho family GTPase |
| Interleukin-2 inducible T cell kinase (ITK) | 4710 | PF 06465469 | Potent inhibitor of ITK |
| IκB Kinase | 4806 | BMS 345541 | Selective allosteric inhibitor of IKK; anti-inflammatory |
| MEK | 4192 | PD 0325901 | Potent inhibitor of MEK1/2 |
| mTOR | 5264 | Temsirolimus | mTOR inhibitor |
| NFAT | 3930 | NFAT Inhibitor | Inhibitor of calcineurin-mediated NFAT activation |
| p38 | 1402 | SB 203580 hydrochloride | Selective inhibitor of p38 MAPK |
| PI 3-Kinase | 4674 | CZC 24832 | Selective inhibitor of PI 3-kinase γ |
| PP1 and PP2A | 1336 | Calyculin A | Protein phosphatase 1 and 2A inhibitor |
| Src Family Kinases | 5413 | WH-4-023 | Potent and selective Lck and Src inhibitor; also inhibits SIK |
| Syk Kinase | 2471 | ER 27319 maleate | Selective Syk kinase inhibitor |
Learn More About Our Small Molecules
Validate
ELISAs
Trust the most-referenced ELISAs on the market to verify your antibody array and/or Luminex assay results. R&D Systems offers a wide variety of complete ELISA kits and ELISA Development Systems that have been exhaustively tested for superior quality and reproducibility.
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Linearity of the Human IFN-gamma Quantikine® ELISA Kit (Catalog # DIF50). To assess the linearity of the assay, five samples were spiked with high concentrations of IFN-gamma in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Learn More About Our ELISAs |
Antibodies
Verify antibody array and Luminex assay results and generate publication-quality data with antibodies from R&D Systems. Our antibodies are manufactured and carefully tested with quality and performance as the top priorities. Learn more about what makes our antibodies the best.
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| Detection of IFN-gamma in Human PBMCs. Human PBMCs were stimulated with PMA (Catalog # 1201) and ionomycin. IFN-gamma was detected using Human IFN-gamma Monoclonal Antibody (Catalog # MAB285). Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). | Detection of TNF-alpha in Mouse T Cells. TNF-alpha was detected in immersion fixed activated mouse T cells using Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). Cells were stained (red) and counterstained (green). | Detection of Phospho-p38 by Western blot. Lysates of HepG2 human hepatocellular carcinoma cells were untreated (-) or treated (+) with 20 ng/mL Recombinant Human IL-1 beta (Catalog # 201-LB) for the indicated times. The PVDF membrane was probed with Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). |
ELISpot Kits and Development Modules
Identify and measure the frequency of cytokine secreting cells with the most accurate and sensitive ELISpot kits available. Our single and dual-color complete ELISpot kits are ready-to-run out of the box and require no assay development. Alternatively, we offer ELISpot Development Modules that contain the basic components required to develop your own ELISpot assay.
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Identification of IFN-gamma- and IL-17-Secreting Cells. IFN-gamma (blue spots) and IL-17 (red spots) were secreted from mouse splenocytes stimulated with PMA/Ca2+ ionomycin. Spots of cytokine secretion were visualized using the Mouse IFN-gamma/IL-17 Dual-Color ELISpot Kit (Catalog # ELD5007). View All ELISpots |