Temsirolimus

Biological Activity

Temsirolimus is a mTOR inhibitor; inhibits tumor growth in breast cancer cell lines (IC₅₀ values are 1.6 and 4.3 nM for SKBr3 and BT474, respectively). Inhibits HIF-1α-mediated VEGF production in breast cancer cell lines (BT474 and MDA-MB-231). Directly inhibits serum and VEGF mediated endothelial cell proliferation and morphogenesis in vitro and vessel formation in vivo. Causes G₁/S cell cycle arrest in multiple cancer cell lines. Antiangiogenic. Activates autophagy.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Background References

1. Inhibition of mammalian target of rapamycin signaling by CCI-779 (temsirolimus) induces growth inhibition and cell cycle arrest in Cashmere goat fetal fibroblasts (Capra hircus).
   Wang et al.
   DNA Cell Biol., 2012;31:1095
2. Effects of the mammalian target of rapamycin inhibitor CCI-779 used alone or with chemotherapy on human prostate cancer cells and xenografts.
   Wu L et al.
   Cancer Res., 2005;65:2825
3. The novel mTOR inhibitor CCI-779 (temsirolimus) induces antiproliferative effects through inhibition of mTOR in Bel-7402 liver cancer cells.
   Li et al.
   Cancer Cell Int., 2013;13
4. Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles.
   Galluzzi et al.
   Nat.Rev.Drug.Discov., 2017;
5. Antiangiogenic potential of the Mammalian target of rapamycin inhibitor temsiro.
   Del Bufalo et al.
   Cancer Res., 2006;66:5549

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