Rat beta-NGF Antibody

Catalog #: AF-556-NA
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-556-NA
AF-556-SP

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All beta-NGF Antibodies

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat beta-NGF Antibody Summary

Specificity

Detects rat beta -NGF in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) beta ‑NGF, rhBDNF, recombinant rat (rr) GDNF, rhCNTF, rrGDNF R alpha, rrCNTF, rhGDNF, rhNT-3 and rhNT‑4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat beta -NGF (R&D Systems, Catalog # 556-NG)
Ser122-Gly241
Accession # P25427

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat beta -NGF (Catalog # 556-NG)

Immunohistochemistry

5-15 µg/mL

See below

Rat beta -NGF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Rat beta-NGF Biotinylated Antibody (Catalog # BAF556)

Standard: Recombinant Rat beta-NGF Protein (Catalog # 556-NG)

Neutralization

Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND₅₀) is typically 0.02-0.1 µg/mL in the presence of 3 ng/mL Recombinant Rat beta ‑NGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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beta -NGF in Rat Brain. beta -NGF was detected in perfusion fixed frozen sections of rat brain using Rat beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization

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Cell Proliferation Induced by beta ‑NGF and Neutralization by Rat beta ‑NGF Antibody. Recombinant Rat beta -NGF (Catalog # 556-NG) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat beta -NGF (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA). The ND₅₀ is typically 0.02-0.1 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta -NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name

beta Nerve Growth Factor

Entrez Gene IDs

4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)

Alternate Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Rat beta-NGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Wogonin, a Bioactive Ingredient from Huangqi Guizhi Formula, Alleviates Discogenic Low Back Pain via Suppressing the Overexpressed NGF in Intervertebral Discs
Authors: J Li, W Duan, S Chai, Y Luo, Y Ma, N Yang, M Liu, W He
Mediators of Inflammation, 2023-02-20;2023(0):4436587.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Intranasal Administration of Forskolin and Noopept Reverses Parkinsonian Pathology in PINK1 Knockout Rats
Authors: RK Dagda, RY Dagda, E Vazquez-Ma, B Martinez, A Gallahue
International Journal of Molecular Sciences, 2022-12-30;24(1):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Knockdown of astrocytic Grin2a aggravates beta-amyloid-induced memory and cognitive deficits through regulating nerve growth factor
Authors: Z Du, Y Song, X Chen, W Zhang, G Zhang, H Li, L Chang, Y Wu
Aging Cell, 2021-07-22;0(0):e13437.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot
Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
J Neuroinflammation, 2012-07-23;9(0):181.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr
Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness).
Authors: Murase S, Terazawa E, Queme F, Ota H, Matsuda T, Hirate K, Kozaki Y, Katanosaka K, Taguchi T, Urai H, Mizumura K
J. Neurosci., 2010-03-10;30(10):3752-61.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization
Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR
J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization