Mouse Chordin Antibody

Catalog #: MAB7582 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7582-100
MAB7582-SP

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Mouse Chordin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Chordin in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 119726

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Chordin
Thr27-Ser948
Accession # Q9Z0E2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Chordin DuoSet ELISA Kit (Catalog # DY758) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Mouse Chordin ELISA Standard Curve. Recombinant Mouse Chordin protein was serially diluted 2-fold and captured by Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin

Chordin is a secreted glycoprotein that regulates dorsoventral patterning during gastrulation. Chordin functions as a bone morphogenetic protein (BMP) antagonist that blocks their ventralizing activity by binding to the BMPs and inhibiting their interaction with their receptors. Mouse Chordin cDNA encodes a 948 amino acid (aa) precursor protein with a putative 26 aa signal peptide. Chordin contains four internal cysteine-rich repeats (CRs) that are conserved in the spacing of their ten cysteine residues. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding. These conserved CRs are present in an expanding family of secreted molecules that antagonize BMP signaling. Xolloid (an extracellular zinc metalloproteinase) can cleave chordin at two specific sites resulting in chordin fragments with lower BMP-affinity. Cleavage of the chordin/BMP complex can reverse the BMP antagonist activity of chordin. Mouse Chordin is expressed at high levels in 7 day postcoitum mouse embryos. Chordin expression is also detected in multiple fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles for Chordin in organogenesis and homeostasis.

References