Mouse Chordin Antibody

Catalog # Availability Size / Price Qty
MAB758
MAB758-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse Chordin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Chordin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Pref-1 or rmWIF-1 is observed.
Source
Monoclonal Rat IgG2A Clone # 119721
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Chordin
Thr27-Ser948
Accession # Q9Z0E2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Chordin (Catalog # 758-CN) under non-reducing conditions only

Mouse Chordin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Chordin Biotinylated Antibody (Catalog # BAF758)

Standard: Recombinant Mouse Chordin Protein (Catalog # 758-CN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin

Chordin is a secreted glycoprotein that regulates dorsoventral patterning during gastrulation. Chordin functions as a bone morphogenetic protein (BMP) antagonist that blocks their ventralizing activity by binding to the BMPs and inhibiting their interaction with their receptors. Mouse Chordin cDNA encodes a 948 amino acid (aa) precursor protein with a putative 26 aa signal peptide. Chordin contains four internal cysteine-rich repeats (CRs) that are conserved in the spacing of their ten cysteine residues. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding. These conserved CRs are present in an expanding family of secreted molecules that antagonize BMP signaling. Xolloid (an extracellular zinc metalloproteinase) can cleave chordin at two specific sites resulting in chordin fragments with lower BMP-affinity. Cleavage of the chordin/BMP complex can reverse the BMP antagonist activity of chordin. Mouse Chordin is expressed at high levels in 7 day postcoitum mouse embryos. Chordin expression is also detected in multiple fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles for Chordin in organogenesis and homeostasis.

References
  1. De Robertis, E.M. and Y. Sasai (1996) Nature 380:37.
  2. Larrain, J. et al. (2000) Development 127:821.
  3. Coffinier, C. et al. (2001) Mech. Dev. 100:119.
Entrez Gene IDs
8646 (Human); 12667 (Mouse)
Alternate Names
Chordin; CHRD; MGC133038; Sog

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Citation for Mouse Chordin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A concentration-dependent endocytic trap and sink mechanism converts Bmper from an activator to an inhibitor of Bmp signaling.
    Authors: Kelley R, Ren R, Pi X, Wu Y, Moreno I, Willis M, Moser M, Ross M, Podkowa M, Attisano L, Patterson C
    J. Cell Biol., 2009-02-16;184(4):597-609.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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