Mouse beta -NGF Antibody

Catalog #: MAB11561 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11561-100
MAB11561-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse beta -NGF Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse beta ‑NGF in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2529A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived mouse beta ‑NGF
Ser122-Gly241
Accession # Q6LDU8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Mouse beta ‑NGF Monoclonal Antibody (Catalog # MAB1156).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Mouse beta ‑NGF ELISA Standard Curve. Recombinant Mouse beta -NGF protein was serially diluted 2-fold and captured by Rabbit Anti-Mouse beta -NGF Monoclonal Antibody (Catalog # MAB1156) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Mouse beta -NGF Monoclonal Antibody (Catalog # MAB11561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2). beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF (6).

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).

References

Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
Angeletti, R H. et al. (1973) Biochemistry 12:100.
Ullrich, A. et al. (1983) Nature 303:821.
Gotz, R. et al. (1994) Nature 372:266.
Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.

Long Name

beta Nerve Growth Factor

Entrez Gene IDs

4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)

Alternate Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428