Mouse BAMBI/NMA Antibody

Name: Mouse BAMBI/NMA Antibody
Brand: R&D Systems
SKU: AF2387
Rating: 4.3 (3 reviews)

Catalog #: AF2387
3 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2387
AF2387-SP

Detection of Mouse BAMBI/NMA by Western Blot.

1 Image

All BAMBI/NMA Antibodies

Product Details

Citations (3)

FAQs

Supplemental Products

Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BAMBI/NMA Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse BAMBI/NMA in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human BAMBI is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BAMBI/NMA Ile28-Thr143 Accession # Q9D0L6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse BAMBI/NMA by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2387) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAMBI/NMA at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: BAMBI/NMA

BAMBI/NMA is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family protein but has a short cytoplasmic region that lacks the kinase domain. It functions as a membrane-bound decoy receptor by competing with type I receptors to form a heterodimer with type II receptors. Mouse BAMBI/NMA share approximately 93% amino acid sequence homology with human BAMBI/NMA.

Long Name

BMP and Activin Membrane-bound Inhibitor

Entrez Gene IDs

25805 (Human); 68010 (Mouse)

Alternate Names

BAMBI; BMP and activin membrane-bound inhibitor homolog (Xenopus laevis); BMP and activin membrane-bound inhibitor homolog; NMA; NMANon-metastatic gene A protein; Putative transmembrane protein NMA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse BAMBI/NMA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
PLoS ONE, 2010-09-24;5(9):e12995.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot
BAMBI (bone morphogenetic protein and activin membrane-bound inhibitor) reveals the involvement of the transforming growth factor-beta family in pain modulation.
Authors: Tramullas M, Lantero A, Diaz A, Morchon N, Merino D, Villar A, Buscher D, Merino R, Hurle JM, Izpisua-Belmonte JC, Hurle MA
J. Neurosci., 2010-01-27;30(4):1502-11.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
TLR4 enhances TGF-beta signaling and hepatic fibrosis.
Authors: Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF
Nat. Med., 2007-10-21;13(11):1324-32.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot