Human Sortilin Biotinylated Antibody Summary
Ser78-Asn755
Accession # Q99523
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sortilin
Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10p-D) family of sorting receptors (1, 2). Human preprosortilin is processed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pH-dependent high affinity binding that blocks the Sortilin ligand binding site both pre‑ and post-cleavage (3). The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10 CC) essential for ligand binding (2). The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, endocytosis and Golgi-endosome transport; as little as 10% may be found on the cell surface (4). Mature human Sortilin shares 91% aa identity with mouse and rat Sortilin and 93% aa identity with dog. During murine development, sortilin is mainly expressed in the nervous system (5) where it is a receptor for neuropeptides including neurotensin, nerve growth factor (NGF) and brain‑derived neurotrophic factor (BDNF) (6-9). ProNGF (or the NGF propeptide alone) binds sortilin with much higher affinity (KD ~5-8 nM) than does mature NGF (KD ~90 nM). The complex of sortilin, pro-NGF and the receptor p75ntr results in endocytosis of proNGF and induction of apoptosis (7). Similar results have been obtained with pro-BDNF and BDNF (8, 9). Sortilin is expressed in other tissues including testis, skeletal muscle and fat (1, 10). It is essential and sufficient for biogenesis of Glut4 storage vesicles necessary for insulin responsiveness in adipocytes (10). Sortilin also binds lipoprotein lipase (11), apoE (2) and RAP (1, 11). Binding is competitive, indicating that although unrelated, targets likely bind the same site.
- Petersen, C.M. et al. (1997) J. Biol. Chem. 272:3599.
- Westergaard, U.B. et al. (2004) J. Biol. Chem. 279:50221.
- Petersen, C.M. et al. (1998) EMBO J. 18:595.
- Nielsen, M.S. et al. (2001) EMBO J. 20:2180.
- Hermans-Borgmeyer, I. et al. (1999) Mol. Brain Res. 65:216.
- Mazella, J. et al. (1998) J. Biol. Chem. 273:26273.
- Nykjaer, A. et al. (2004) Nature 427:843.
- Teng, H.K. et al. (2005) J. Neurosci. 25:5455.
- Chen, Z-Y. et al. (2004) J. Neurosci. 25:6156.
- Shi, J. and K.V. Kandror (2005) Dev. Cell 9:99.
- Nielsen, M.S. et al. (1999) J. Biol. Chem. 274:8832.
Product Datasheets
Citation for Human Sortilin Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Anti-sortilin1 Antibody Up-Regulates Progranulin via Sortilin1 Down-Regulation
Authors: S Miyakawa, H Sakuma, D Warude, S Asanuma, N Arimura, T Yoshihara, D Tavares, A Hata, K Ida, Y Hori, Y Okuzono, S Yamamoto, K Iida, H Shimizu, S Kondo, S Sato
Frontiers in Neuroscience, 2020-12-15;14(0):586107.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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