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Human PDGF R beta APC-conjugated Antibody

Catalog #: FAB1263A
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FAB1263A
Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry.
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Human PDGF R beta APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF Receptor beta -subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF Receptor alpha ‑subunit. Detects the PDGF Receptor beta -subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.
Source
Monoclonal Mouse IgG1 Clone # PR7212
Purification
Protein A or G purified from ascites
Immunogen
Human skin fibroblast membrane extracts
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PDGF R beta antibody in MG-63 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta APC-conjugated Monoclonal Antibody (Catalog # FAB1263A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is a potent PDGF antagonist (4).

References
  1. Hart et al. (1987) J. Biol. Chem. 262:10780.
  2. Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
  3. Seifert et al. (1989) J. Biol. Chem. 264:8771.
  4. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
Long Name
Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs
5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

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Citation for Human PDGF R beta APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Platelet-Derived Growth Factor Receptor-Positive Pericytic Cells of White Adipose Tissue from Critical Limb Ischemia Patients Display Mesenchymal Stem Cell-Like Properties
    Authors: EJ Kim, SG Seo, HS Shin, DJ Lee, JH Kim, DY Lee
    Clin Orthop Surg, 2017-05-08;9(2):239-248.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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