Human/Mouse/Rat Sortilin Antibody Summary
Ser78-Asn755
Accession # Q99523
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human Sortilin antibody by Western Blot. Detection of Human Sortilin antibody by Western Blot.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Sortilin_MAB3154_Western_Blot_17568.jpg)
Detection of Human Sortilin by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Detection of Human Sortilin antibody by Simple Western<sup>TM</sup>. Detection of Human Sortilin antibody by Simple Western<sup>TM</sup>.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Sortilin_MAB3154_Western_Blot_17497.jpg)
Detection of Human Sortilin by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 87 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
![Detection of Mouse and Rat Sortilin antibody by Western Blot. Detection of Mouse and Rat Sortilin antibody by Western Blot.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Sortilin_MAB3154_Western_Blot_19991.jpg)
Detection of Mouse and Rat Sortilin by Western Blot. Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Western Blot Shows Human Sortilin Antibody Specificity by Using Knockout Cell Line. Western Blot Shows Human Sortilin Antibody Specificity by Using Knockout Cell Line.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Sortilin_MAB3154_Knockout_Validated_22533.jpg)
Western Blot Shows Human Sortilin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Sortilin knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sortilin
Sortilin, also known as neurotensin receptor 3, is a 100 kDa transmembrane receptor that is localized both in the endosomal compartment and on plasma membranes. It contains an extracellular/lumenal Vps10p (vacuolar protein-sorting 10 protein) domain and is one of five known members of the mammalian Vps10p-D receptor family. A Sortilin propeptide, cleaved by a furin, blocks premature ligand binding to uncleaved Sortilin and functions as a chaperone to facilitate trafficking in the Golgi compartments. Sortilin binds multiple ligands and has diverse functions. Mature human Sortilin extracellular domain shares 92% and 91% amino acid sequence identity with that of mouse and rat Sortilin, respectively.
Product Datasheets
Citation for Human/Mouse/Rat Sortilin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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A proteomic survey indicates sortilin as a secondary substrate of the ER translocation inhibitor cyclotriazadisulfonamide (CADA)
Authors: Victor Van Puyenb
Mol. Cell Proteomics, 2016-12-20;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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