Home / Follistatin-like 1/FSTL1 / Human Follistatin-like 1/FSTL1 Antibody

Human Follistatin-like 1/FSTL1 Antibody

Catalog #: AF1694
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1694
AF1694-SP
Detection of Human Follistatin‑like 1/FSTL1 by Western Blot.
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Citations (12)
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Human Follistatin-like 1/FSTL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant mouse FSTL1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Follistatin-like 1/FSTL1
Met1-Ile308
Accession # Q12841
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
Immersion fixed paraffin-embedded sections of human placenta
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Follistatin‑like 1/FSTL1 antibody by Western Blot. View Larger

Detection of Human Follistatin‑like 1/FSTL1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Follistatin-like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Follistatin-like 1/FSTL1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Follistatin-like 1/FSTL1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Follistatin-like 1/FSTL1 in HeLa Human Cell Line. Follistatin-like 1/FSTL1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Follistatin-like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

Follistatin‑like 1/FSTL1 in Human Placenta. Follistatin‑like 1/FSTL1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human Follistatin‑like 1/FSTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1694) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in chorionic villi. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-like 1/FSTL1

Follistatin-like 1 (FSL1 or FSTL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The human FSTL1 cDNA encodes 308 amino acids (aa), including a 20 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently non-functional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature human FSTL1 shares 94%, 95%, 98%, and 99% aa identity with mouse, rat, bovine, and equine FSTL1, respectively. FSTL1 was first identified as a TGF-beta-induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In humans, FSTL1 is a common rheumatoid arthritis auto-antigen (2). It is reported to be either pro-inflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (6-9). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (10, 11). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (11, 12). FSTL1 also appears to be a tumor suppressor, showing down-regulated expression in many human cancers (4, 14, 15). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (14).

References
  1. Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
  2. Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
  3. Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
  4. Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
  5. Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
  6. Clutter, S.D. et al. (2009) J. Immunol. 182:234.
  7. Miyamae, T. et al. (2006) J. Immunol. 177:4758.
  8. Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
  9. Tanaka, M. et al. (2003) Int. Immunol. 15:71.
  10. Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
  11. Oshima, Y. et al. (2008) Circulation 117:3099.
  12. Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
  13. Widera, C. et al. (2009) Clin. Chem. 55:1794.
  14. Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
  15. Mashimo, J. et al. (1997) Cancer Lett. 113:213.
Entrez Gene IDs
11167 (Human); 14314 (Mouse); 79210 (Rat)
Alternate Names
Flik; FLJ50214; Follistatin-like 1; Follistatin-like protein 1; follistatin-related protein 1; Follistatin-related Protein; FRP; FRPFLJ52277; FSL1; FSTL1; TSC-36

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Citations for Human Follistatin-like 1/FSTL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Effects of FSTL1 on cell proliferation in breast cancer cell line MDA?MB?231 and its brain metastatic variant MDA?MB?231?BR
    Authors: J An, L Wang, Y Zhao, Q Hao, Y Zhang, J Zhang, C Yang, L Liu, W Wang, D Fang, T Lu, Y Gao
    Oncol. Rep., 2017-09-26;38(5):3001-3010.
  2. The Expression of Follistatin-like 1 Protein Is Associated with the Activation of the EMT Program in Sj�gren's Syndrome
    Authors: M Sisto, D Ribatti, G Ingravallo, S Lisi
    Journal of Clinical Medicine, 2022-09-13;11(18):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: IHC
  3. Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/beta-catenin signaling in obstructed kidneys in vivo
    Authors: Y Zhang, Y Wang, G Zheng, Y Liu, J Li, H Huang, C Xu, Y Zeng, X Zhang, J Qin, C Dai, HO Hambrock, U Hartmann, B Feng, KK Mak, Y Liu, HY Lan, Y Huang, ZH Zheng, Y Xia
    The Journal of Biological Chemistry, 2022-05-04;0(0):102010.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  4. FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival
    Authors: M Wu, Y Ding, N Wu, J Jiang, Y Huang, F Zhang, H Wang, Q Zhou, Y Yang, W Zhuo, L Teng
    American journal of cancer research, 2021-03-01;11(3):712-728.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  5. Deficiency of Follistatin-Like Protein 1 Accelerates the Growth of Breast Cancer Cells at Lung Metastatic Sites
    Authors: Ying Zhang, Xiaozhou Xu, Ying Yang, Jie Ma, Lulu Wang, Xiangzhi Meng et al.
    Journal of Breast Cancer
  6. Follistatin like-1 aggravates silica-induced mouse lung injury
    Authors: Y Fang, S Zhang, X Li, F Jiang, Q Ye, W Ning
    Sci Rep, 2017-03-24;7(1):399.
    Species: Human
    Sample Types: Serum, Whole Tissue
    Applications: ELISA Development (Capture), IHC
  7. Follistatin-like protein 1 enhances NLRP3 inflammasome-mediated IL-1beta secretion from monocytes and macrophages.
    Authors: Chaly Y, Fu Y, Marinov A, Hostager B, Yan W, Campfield B, Kellum J, Bushnell D, Wang Y, Vockley J, Hirsch R
    Eur J Immunol, 2014-02-20;44(5):1467-79.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Follistatin-like Protein 1 and the Ferritin/Erythrocyte Sedimentation Rate Ratio Are Potential Biomarkers for Dysregulated Gene Expression and Macrophage Activation Syndrome in Systemic Juvenile Idiopathic Arthritis
    Authors: Mark Gorelik, Ndate Fall, Mekibib Altaye, Michael G. Barnes, Susan D. Thompson, Alexei A. Grom et al.
    The Journal of Rheumatology
  9. Plasma Follistatin-Like Protein 1 is Elevated in Kawasaki Disease and May Predict Coronary Artery Aneurysm Formation
    Authors: Mark Gorelik, David C. Wilson, Yona K. Cloonan, Stanford T. Shulman, Raphael Hirsch
    The Journal of Pediatrics
  10. Follistatin-like Protein 1 Promotes Arthritis by Enhancing Inflammatory Cytokine/Chemokine Expression
    Authors: Yury Chaly, Anthony D. Marinov, Leif Oxburgh, Daniel S. Bushnell, Raphael Hirsch
    Arthritis & Rheumatism
  11. Follistatin-like protein 1 is a mesenchyme-derived inflammatory protein and may represent a biomarker for systemic-onset juvenile rheumatoid arthritis
    Authors: David C. Wilson, Anthony D. Marinov, Harry C. Blair, Daniel S. Bushnell, Susan D. Thompson, Yury Chaly et al.
    Arthritis & Rheumatism
  12. Tumor suppressor effect of follistatin-like 1 in ovarian and endometrial carcinogenesis: a differential expression and functional analysis.
    Authors: Chan QK, Ngan HY, Ip PP, Liu VW, Xue WC, Cheung AN
    Carcinogenesis, 2008-09-16;30(1):114-21.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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