Home / Follistatin-like 1/FSTL1 / Human Follistatin-like 1/FSTL1 Antibody

Human Follistatin-like 1/FSTL1 Antibody

Catalog #: MAB1694
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1694
MAB1694-SP
Product Details
Citations (6)
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Human Follistatin-like 1/FSTL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse FSTL1 is observed.
Source
Monoclonal Rat IgG2b Clone # 229007
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Follistatin-like 1/FSTL1 (Catalog # 1694-FN)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Follistatin-like 1/FSTL1

Follistatin-like 1 (FSL1 or FSTL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The human FSTL1 cDNA encodes 308 amino acids (aa), including a 20 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently non-functional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature human FSTL1 shares 94%, 95%, 98% and 99% aa identity with mouse, rat, bovine and equine FSTL1, respectively. FSTL1 was first identified as a TGF-beta-induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In humans, FSTL1 is a common rheumatoid arthritis auto-antigen (2). It is reported to be either pro-inflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (6-9). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (10, 11). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (11, 12). FSTL1 also appears to be a tumor suppressor, showing down-regulated expression in many human cancers (4, 14, 15). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (14).

References
  1. Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
  2. Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
  3. Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
  4. Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
  5. Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
  6. Clutter, S.D. et al. (2009) J. Immunol. 182:234.
  7. Miyamae, T. et al. (2006) J. Immunol. 177:4758.
  8. Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
  9. Tanaka, M. et al. (2003) Int. Immunol. 15:71.
  10. Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
  11. Oshima, Y. et al. (2008) Circulation 117:3099.
  12. Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
  13. Widera, C. et al. (2009) Clin. Chem. 55:1794.
  14. Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
  15. Mashimo, J. et al. (1997) Cancer Lett. 113:213.
Entrez Gene IDs
11167 (Human); 14314 (Mouse); 79210 (Rat)
Alternate Names
Flik; FLJ50214; Follistatin-like 1; Follistatin-like protein 1; follistatin-related protein 1; Follistatin-related Protein; FRP; FRPFLJ52277; FSL1; FSTL1; TSC-36

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Citations for Human Follistatin-like 1/FSTL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Follistatin-like 1 promotes proliferation of matured human hypoxic iPSC-cardiomyocytes and is secreted by cardiac fibroblasts
    Authors: Marijn C. Peters, Sofia Di Martino, Thomas Boelens, Jiabin Qin, Alain van Mil, Pieter A. Doevendans et al.
    Molecular Therapy - Methods & Clinical Development
  2. Follistatin like-1 aggravates silica-induced mouse lung injury
    Authors: Y Fang, S Zhang, X Li, F Jiang, Q Ye, W Ning
    Sci Rep, 2017-03-24;7(1):399.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  3. Epicardial FSTL1 reconstitution regenerates the adult mammalian heart.
    Authors: Wei K, Serpooshan V, Hurtado C, Diez-Cunado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider M, van den Hoff M, Butte M, Yang P, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P
    Nature, 2015-09-16;525(7570):479-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Follistatin-like Protein 1 and the Ferritin/Erythrocyte Sedimentation Rate Ratio Are Potential Biomarkers for Dysregulated Gene Expression and Macrophage Activation Syndrome in Systemic Juvenile Idiopathic Arthritis
    Authors: Mark Gorelik, Ndate Fall, Mekibib Altaye, Michael G. Barnes, Susan D. Thompson, Alexei A. Grom et al.
    The Journal of Rheumatology
  5. Plasma Follistatin-Like Protein 1 is Elevated in Kawasaki Disease and May Predict Coronary Artery Aneurysm Formation
    Authors: Mark Gorelik, David C. Wilson, Yona K. Cloonan, Stanford T. Shulman, Raphael Hirsch
    The Journal of Pediatrics
  6. Follistatin-like Protein 1 Promotes Arthritis by Enhancing Inflammatory Cytokine/Chemokine Expression
    Authors: Yury Chaly, Anthony D. Marinov, Leif Oxburgh, Daniel S. Bushnell, Raphael Hirsch
    Arthritis & Rheumatism

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