Human BMP-1/PCP Antibody

Catalog #: MAB19271 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19271-100
MAB19271-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-1/PCP Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP-1/PCP in direct ELISA.

Source

Monoclonal Rat IgG_2A Clone # 264801

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP‑1/PCP
Ala121-Gln730
Accession # NP_001190

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of BMP‑1/PCP in Human Kidney. BMP‑1/PCP was detected in immersion fixed paraffin-embedded sections of Human Kidney using Rat Anti-Human BMP‑1/PCP Monoclonal Antibody (Catalog # MAB19271) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-1/PCP

Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5).

References

Wozney, J.M. et al. (1988) Science 242:1528.
Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
Leighton, M. and K.E. Kadler (2003) J. Biol. Chem. 278:18478.
Hartigan, N. et al. (2003) J. Biol. Chem. 278:18045.

Long Name

Bone Morphogenetic Protein 1

Entrez Gene IDs

649 (Human); 12153 (Mouse); 83470 (Rat)

Alternate Names

BMP1; BMP-1; bone morphogenetic protein 1; EC 3.4.24; EC 3.4.24.19; FLJ44432; Mammalian tolloid protein; mTld; PCOLC; PCP; PCP2; procollagen C-endopeptidase; Procollagen C-proteinase; TLD