Human beta-NGF Antibody

Catalog #: MAB256
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB256-SP
MAB256-100
MAB256-500

Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody.

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All beta-NGF Antibodies

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Citations (7)

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Human beta-NGF Antibody Summary

Specificity

Detects human beta ‑NGF in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) beta ‑NGF and recombinant rat (rr) beta ‑NGF is observed and less than 5% cross-reactivity with recombinant human (rh) BDNF, rhNT‑3, and rhNT‑4 is observed. This antibody neutralizes the bioactivity of rh beta -NGF, rm beta -NGF, and rr beta -NGF.

Source

Monoclonal Mouse IgG₁ Clone # 25623

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta -NGF
Ser122-Ala241
Accession # CAA36832

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human beta -NGF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human beta ‑NGF Biotinylated Antibody (Catalog # BAF256)

Standard: Recombinant Human beta-NGF Protein (Catalog # 256-GF)

Neutralization

Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 5 ng/mL Recombinant Human beta ‑NGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. Recombinant Human beta -NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta -NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human beta -NGF Monoclonal Antibody (Catalog # MAB256). The ND₅₀ is typically 0.1-0.4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name

beta Nerve Growth Factor

Entrez Gene IDs

4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)

Alternate Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

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Product Datasheet

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Related Research Areas

Citations for Human beta-NGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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The Role of Nerve Growth Factor in Maintaining Proliferative Capacity, Colony-Forming Efficiency, and the Limbal Stem Cell Phenotype
Authors: S Kolli, S Bojic, AE Ghareeb, M Kurzawa-Ak, FC Figueiredo, M Lako
Stem Cells, 2019-01-01;37(1):139-149.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
RIP2 Gates TRAF6 Interaction with Death Receptor p75NTR to Regulate Cerebellar Granule Neuron Survival
Authors: L Kisiswa, D Fernández-, MC Sergaki, CF Ibáñez
Cell Rep, 2018-07-24;24(4):1013-1024.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: ICC, Western Blot
Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
PLoS ONE, 2017-01-09;12(1):e0168565.
Species: Human
Sample Types: Serum
Applications: ELISA Development
Functional Characterization of Human ProNGF and NGF Mutants: Identification of NGF P61SR100E as a "Painless" Lead Investigational Candidate for Therapeutic Applications.
Authors: Malerba F, Paoletti F, Bruni Ercole B, Materazzi S, Nassini R, Coppi E, Patacchini R, Capsoni S, Lamba D, Cattaneo A
PLoS ONE, 2015-09-15;10(9):e0136425.
Species: Human
Sample Types: Protein
Applications: Surface Plasmon Resonance
Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
Authors: Samah B, Porcheray F, Gras G
Clin. Exp. Immunol., 2008-01-08;151(3):476-86.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Nerve growth factor induces increased airway inflammation via a neuropeptide-dependent mechanism in a transgenic animal model of allergic airway inflammation.
Authors: Quarcoo D, Schulte-Herbruggen O, Lommatzsch M, Schierhorn K, Hoyle GW, Renz H, Braun A
Clin. Exp. Allergy, 2004-07-01;34(7):1146-51.
Species: Transgenic Mouse
Sample Types: BALF
Applications: ELISA Development
Nerve growth factor overexpression and autocrine loop in breast cancer cells.
Authors: Dolle L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H
Oncogene, 2003-08-28;22(36):5592-601.
Species: Human
Sample Types: Cell Culture Supernates, Cell Lysates, Whole Cells, Whole Tissue
Applications: ELISA Development, ICC, IHC-P, Neutralization, Western Blot