Human beta-NGF Antibody Summary
Ser122-Ala241
Accession # CAA36832
Applications
Human beta -NGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/beta-NGF_MAB256_Block_Neutralize_9103.jpg)
Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. Recombinant Human beta -NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta -NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human beta -NGF Monoclonal Antibody (Catalog # MAB256). The ND50 is typically 0.1-0.4 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-NGF
NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.
NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
Product Datasheets
Citations for Human beta-NGF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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The Role of Nerve Growth Factor in Maintaining Proliferative Capacity, Colony-Forming Efficiency, and the Limbal Stem Cell Phenotype
Authors: S Kolli, S Bojic, AE Ghareeb, M Kurzawa-Ak, FC Figueiredo, M Lako
Stem Cells, 2019-01-01;37(1):139-149.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
RIP2 Gates TRAF6 Interaction with Death Receptor p75NTR to Regulate Cerebellar Granule Neuron Survival
Authors: L Kisiswa, D Fernández-, MC Sergaki, CF Ibáñez
Cell Rep, 2018-07-24;24(4):1013-1024.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: ICC, Western Blot -
Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
PLoS ONE, 2017-01-09;12(1):e0168565.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Functional Characterization of Human ProNGF and NGF Mutants: Identification of NGF P61SR100E as a "Painless" Lead Investigational Candidate for Therapeutic Applications.
Authors: Malerba F, Paoletti F, Bruni Ercole B, Materazzi S, Nassini R, Coppi E, Patacchini R, Capsoni S, Lamba D, Cattaneo A
PLoS ONE, 2015-09-15;10(9):e0136425.
Species: Human
Sample Types: Protein
Applications: Surface Plasmon Resonance -
Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
Authors: Samah B, Porcheray F, Gras G
Clin. Exp. Immunol., 2008-01-08;151(3):476-86.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Nerve growth factor induces increased airway inflammation via a neuropeptide-dependent mechanism in a transgenic animal model of allergic airway inflammation.
Authors: Quarcoo D, Schulte-Herbruggen O, Lommatzsch M, Schierhorn K, Hoyle GW, Renz H, Braun A
Clin. Exp. Allergy, 2004-07-01;34(7):1146-51.
Species: Transgenic Mouse
Sample Types: BALF
Applications: ELISA Development -
Nerve growth factor overexpression and autocrine loop in breast cancer cells.
Authors: Dolle L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H
Oncogene, 2003-08-28;22(36):5592-601.
Species: Human
Sample Types: Cell Culture Supernates, Cell Lysates, Whole Cells, Whole Tissue
Applications: ELISA Development, ICC, IHC-P, Neutralization, Western Blot
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