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TGF-beta 3 Antibody

Catalog #: MAB643
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB643-SP
MAB643-500
MAB643-100
TGF-beta 3 in Human Heart.
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Product Details
Citations (3)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

TGF-beta 3 Antibody Summary

Specificity
Detects TGF-beta 3 from multiple species in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) TGF-beta 1or rhTGF-beta 2.
Source
Monoclonal Mouse IgG1 Clone # 44922
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TGF‑ beta 3
Ala301-Ser412 (Tyr340Phe)
Accession # NP_003230.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TGF-beta 3 (Catalog # 243-B3)
under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below

Human TGF-beta 3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: TGF‑ beta 3 Biotinylated Antibody (Catalog # BAF243)

Standard: Recombinant Human TGF-beta 3 Protein (Catalog # 243-B3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TGF-beta 3 antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

TGF-beta 3 in Human Heart. TGF-beta 3 was detected in immersion fixed paraffin-embedded sections of human heart using TGF-beta 3 Monoclonal Antibody (Catalog # MAB643) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 3

The active form of TGF-beta 3 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 3 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.

Long Name
Transforming Growth Factor beta 3
Entrez Gene IDs
7043 (Human); 21809 (Mouse); 25717 (Rat)
Alternate Names
ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3

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Citations for TGF-beta 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis.
    Authors: Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J
    Nat Commun, 2016-03-18;7(0):11012.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Transforming growth factor beta expression in human placenta and placental bed during early pregnancy.
    Authors: Simpson H, 2019, Robson SC, Bulmer JN, Barber A, Lyall F
    6897, 2002-01-01;23(1):44-58.
    Species: Human
    Sample Types: Tissue Homogenates
  3. Blood pressure homeostasis is maintained by a P311–TGF-beta axis
    Authors: Kameswara Rao Badri, Ming Yue, Oscar A. Carretero, Sree Latha Aramgam, Jun Cao, Stephen Sharkady et al.
    Journal of Clinical Investigation

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