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TGF-beta 3 Biotinylated Antibody

Catalog #: BAF243
6 Citations 1 Review Datasheet / COA / SDS
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BAF243
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

TGF-beta 3 Biotinylated Antibody Summary

Specificity
Detects TGF-beta 3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TGF‑ beta 1, rhTGF-beta 1.2, and rhTGF-beta 2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant chicken TGF‑ beta 3 and recombinant human TGF‑ beta 3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human TGF-beta 3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: TGF‑ beta 3 Antibody (Catalog # MAB643)

Standard: Recombinant Human TGF-beta 3 Protein (Catalog # 243-B3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 3

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1 - 7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1 - 4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8 - 10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity.(1) TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription ( 3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

References
  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1988) EMBO J. 7:3737.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name
Transforming Growth Factor beta 3
Entrez Gene IDs
7043 (Human); 21809 (Mouse); 25717 (Rat)
Alternate Names
ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3

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Citations for TGF-beta 3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype
    Authors: N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh
    Anticancer Res., 2018-04-01;38(4):2007-2014.
  2. Muscleblind-like 1 is required for normal heart valve development in vivo
    Authors: Ryan J. Coram, Samantha J. Stillwagon, Anuradha Guggilam, Michael W. Jenkins, Maurice S. Swanson, Andrea N. Ladd
    BMC Developmental Biology
  3. Blood pressure homeostasis is maintained by a P311–TGF-beta axis
    Authors: Kameswara Rao Badri, Ming Yue, Oscar A. Carretero, Sree Latha Aramgam, Jun Cao, Stephen Sharkady et al.
    Journal of Clinical Investigation
  4. Loss of muscleblind-like 1 promotes invasive mesenchyme formation in endocardial cushions by stimulating autocrine TGF beta 3
    Authors: Kathryn E LeMasters, Yotam Blech-Hermoni, Samantha J Stillwagon, Natalie A Vajda, Andrea N Ladd
    BMC Developmental Biology
  5. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
    Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Transforming growth factor beta expression in human placenta and placental bed during early pregnancy.
    Authors: Simpson H, 2019, Robson SC, Bulmer JN, Barber A, Lyall F
    6897, 2002-01-01;23(1):44-58.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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TGF-beta 3 Biotinylated Antibody
By Anonymous on 03/28/2019
Sample Tested: Recombinant protein Species: Human

Direct ELISA to confirm specificity using recombinant protein

TGF-beta 3 Biotinylated Antibody BAF243