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Human beta -NGF Antibody

Catalog #: MAB2561
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2561-SP
MAB2561-500
MAB2561-100
Human beta ‑NGF ELISA Standard Curve.
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Product Details
Citations (5)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta -NGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -NGF in direct ELISAs. In sandwich immunoassays, cross-reactivity with recombinant mouse (rm) beta ‑NGF and recombinant rat (rr) beta ‑NGF has not been determined. In direct ELISAs, 100% cross-reactivity with rm beta ‑NGF and rr beta ‑NGF is observed, and no cross-reactivity with recombinant human (rh) BDNF, rhNT-3, or rhNT-4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 25617
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta -NGF
Ser122-Ala241
Accession # CAA36832
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human beta -NGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human beta ‑NGF Biotinylated Antibody (Catalog # BAF256)

Standard: Recombinant Human beta-NGF Protein (Catalog # 256-GF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA Capture (Matched Antibody Pair) View Larger

Human beta ‑NGF ELISA Standard Curve. Recombinant Human beta ‑NGF protein (256-GF) was serially diluted 2-fold and captured by Mouse Anti-Human beta ‑NGF Monoclonal Antibody (Catalog # MAB2561) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human beta ‑NGF Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF256) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

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Citations for Human beta -NGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Herpes Simplex Virus 2 Counteracts Neurite Outgrowth Repulsion during Infection in a Nerve Growth Factor-Dependent Manner
    Authors: Kai A. Kropp, Alberto Domingo López-Muñoz, Birgit Ritter, Rocío Martín, Alberto Rastrojo, Sangar Srivaratharajan et al.
    Journal of Virology
  2. The Use of TrkA-PathHunter Assay in High-Throughput Screening to Identify Compounds That Affect Nerve Growth Factor Signaling
    Authors: Pontus Forsell, Helena Almqvist, Per Hillertz, Tomas Åkerud, Magdalena Otrocka, Lina Eisele et al.
    J Biomol Screen
  3. The role of nerve growth factor in hyperosmolar stress induced apoptosis.
    Authors: Chang EJ, Im YS, Kay EP
    J. Cell. Physiol., 2008-07-01;216(1):69-77.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Nerve growth factor beta/pro-nerve growth factor and their receptors in normal human oral mucosa.
    Authors: Hayashi K, Storesund T, Schreurs O
    Eur. J. Oral Sci., 2007-10-01;115(5):344-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. A feed-forward loop involving Trib3, Akt and FoxO mediates death of NGF-deprived neurons
    Authors: N Zareen, S C Biswas, L A Greene
    Cell Death & Differentiation

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