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Human beta-NGF Antibody

Catalog #: MAB256
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB256-SP
MAB256-100
MAB256-500
Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody.
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Citations (12)
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Human beta-NGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta ‑NGF in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) beta ‑NGF and recombinant rat (rr)  beta ‑NGF is observed and less than 5% cross-reactivity with recombinant human (rh) BDNF, rhNT‑3, and rhNT‑4 is observed. This antibody neutralizes the bioactivity of rh beta -NGF, rm beta -NGF, and rr beta -NGF.
Source
Monoclonal Mouse IgG1 Clone # 25623
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta -NGF
Ser122-Ala241
Accession # CAA36832
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human beta -NGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human beta ‑NGF Biotinylated Antibody (Catalog # BAF256)

Standard: Recombinant Human beta-NGF Protein (Catalog # 256-GF)

Neutralization
Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 5 ng/mL Recombinant Human beta ‑NGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. View Larger

Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. Recombinant Human beta -NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta -NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human beta -NGF Monoclonal Antibody (Catalog # MAB256). The ND50 is typically 0.1-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

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Citations for Human beta-NGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Extracellular vesicles from neurons promote neural induction of stem cells through cyclin D1
    Authors: Lu Song, Xinran Tian, Randy Schekman
    Journal of Cell Biology
  2. NGF and proNGF Reciprocal Interference in Immunoassays: Open Questions, Criticalities, and Ways Forward
    Authors: Francesca Malerba, Francesca Paoletti, Antonino Cattaneo
    Frontiers in Molecular Neuroscience
  3. Alterations of Tear Neuromediators in Dry Eye Disease
    Authors: Alessandro Lambiase, Alessandra Micera, Marta Sacchetti, Magdalena Cortes, Flavio Mantelli, Stefano Bonini
    Archives of Ophthalmology
  4. The Role of Nerve Growth Factor in Maintaining Proliferative Capacity, Colony-Forming Efficiency, and the Limbal Stem Cell Phenotype
    Authors: S Kolli, S Bojic, AE Ghareeb, M Kurzawa-Ak, FC Figueiredo, M Lako
    Stem Cells, 2019-01-01;37(1):139-149.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. RIP2 Gates TRAF6 Interaction with Death Receptor p75NTR to Regulate Cerebellar Granule Neuron Survival
    Authors: L Kisiswa, D Fernández-, MC Sergaki, CF Ibáñez
    Cell Rep, 2018-07-24;24(4):1013-1024.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: ICC, Western Blot
  6. Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
    Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
    PLoS ONE, 2017-01-09;12(1):e0168565.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  7. Functional Characterization of Human ProNGF and NGF Mutants: Identification of NGF P61SR100E as a "Painless" Lead Investigational Candidate for Therapeutic Applications.
    Authors: Malerba F, Paoletti F, Bruni Ercole B, Materazzi S, Nassini R, Coppi E, Patacchini R, Capsoni S, Lamba D, Cattaneo A
    PLoS ONE, 2015-09-15;10(9):e0136425.
    Species: Human
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  8. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
    Authors: Samah B, Porcheray F, Gras G
    Clin. Exp. Immunol., 2008-01-08;151(3):476-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Nerve growth factor induces increased airway inflammation via a neuropeptide-dependent mechanism in a transgenic animal model of allergic airway inflammation.
    Authors: Quarcoo D, Schulte-Herbruggen O, Lommatzsch M, Schierhorn K, Hoyle GW, Renz H, Braun A
    Clin. Exp. Allergy, 2004-07-01;34(7):1146-51.
    Species: Transgenic Mouse
    Sample Types: BALF
    Applications: ELISA Development
  10. Nerve growth factor overexpression and autocrine loop in breast cancer cells.
    Authors: Dolle L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H
    Oncogene, 2003-08-28;22(36):5592-601.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates, Whole Cells, Whole Tissue
    Applications: ELISA Development, ICC, IHC-P, Neutralization, Western Blot
  11. Changes in vitreal protein profile and retina mRNAs in Reeler mice: NGF, IL33 and Muller cell activation.
    Authors: Balzamino BO, Esposito G, Marino R et al.
    PLoS One.
  12. Stage-Specific Transcription Factors Drive Astrogliogenesis by Remodeling Gene Regulatory Landscapes
    Authors: N Tiwari, A Pataskar, S Péron, S Thakurela, SK Sahu, M Figueres-O, N Marichal, L López-Masc, VK Tiwari, B Berninger
    Cell Stem Cell, 2018-10-04;23(4):557-571.e8.

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