Recombinant Mouse beta-NGF Protein

Name: Recombinant Mouse beta-NGF Protein
Brand: R&D Systems
SKU: 1156-NG
Rating: 5 (1 reviews)

Catalog #: 1156-NG
18 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1156-NG-100/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1156-NG-100

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Product Details

Citations (18)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse beta-NGF Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.1-1 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse beta-NGF protein
Ser122-Gly241

Accession #

Q6LDU8

N-terminal Sequence
Analysis

Ser122

Predicted Molecular Mass

13.5 kDa

Product Datasheets

1156-NG (with carrier)

Product Datasheet

COA

SDS

1156-NG/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1156-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1156-NG/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: beta-NGF

NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2). beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF (6).

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).

References

Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
Angeletti, R H. et al. (1973) Biochemistry 12:100.
Ullrich, A. et al. (1983) Nature 303:821.
Gotz, R. et al. (1994) Nature 372:266.
Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.

Long Name

beta Nerve Growth Factor

Entrez Gene IDs

4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)

Alternate Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

Related Research Areas

Citations for Recombinant Mouse beta-NGF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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GRT-X Stimulates Dorsal Root Ganglia Axonal Growth in Culture via TSPO and Kv7.2/3 Potassium Channel Activation
Authors: El Chemali, L;Boutary, S;Liu, S;Liu, GJ;Middleton, RJ;Banati, RB;Bahrenberg, G;Rupprecht, R;Schumacher, M;Massaad-Massade, L;
International journal of molecular sciences
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay
VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types
Authors: J Sarkar, Y Luo, Q Zhou, E Ivakhnitsk, D Lara, E Katz, MG Sun, V Guaiquil, M Rosenblatt
PLoS ONE, 2022-07-21;17(7):e0269818.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons
Authors: P Pagella, J Catón, CT Meisel, TA Mitsiadis
Cells, 2020-03-06;9(3):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture
A pivotal role of BEX1 in liver progenitor cell expansion in mice
Authors: Y Gu, W Wei, Y Cheng, B Wan, X Ding, H Wang, Y Zhang, M Jin
Stem Cell Res Ther, 2018-06-15;9(1):164.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Excessive nerve growth factor impairs bidirectional communication between the oocyte and cumulus cells resulting in reduced oocyte competence
Authors: Y Zhai, G Yao, F Rao, Y Wang, X Song, F Sun
Reprod. Biol. Endocrinol., 2018-03-27;16(1):28.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Epiregulin and EGFR interactions are involved in pain processing
Authors: LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko
J. Clin. Invest., 2017-08-07;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors
Authors: F Zeng, Z Li, Q Zhu, R Dong, C Zhao, G Li, G Li, W Gao, G Jiang, E Zheng, G Cai, S Moisyadi, J Urschitz, H Yang, D Liu, Z Wu
Sci Rep, 2017-01-24;7(0):41270.
Species: Human
Sample Types: N/A, Whole Cells
Applications: Bioassay, Western Blot
Laguerre Filter Analysis with Partial Least Square Regression Reveals a Priming Effect of ERK and CREB on c-FOS Induction
PLoS ONE, 2016-08-11;11(8):e0160548.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy.
Authors: Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R
PLoS ONE, 2014-02-26;9(2):e89881.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
Treating small fiber neuropathy by topical application of a small molecule modulator of ligand-induced GFRalpha/RET receptor signaling.
Authors: Hedstrom K, Murtie J, Albers K, Calcutt N, Corfas G
Proc Natl Acad Sci U S A, 2014-01-21;111(6):2325-30.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Knockdown of TrkA in cumulus oocyte complexes (COCs) inhibits EGF-induced cumulus expansion by down-regulation of IL-6.
Authors: Wang Y, Liang N, Yao G, Tian H, Zhai Y, Yin Y, Sun F
Mol Cell Endocrinol, 2013-11-08;382(2):804-13.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Oncogenic and drug-sensitive NTRK1 rearrangements in lung cancer.
Authors: Vaishnavi A, Capelletti M, Le A, Kako S, Butaney M, Ercan D, Mahale S, Davies K, Aisner D, Pilling A, Berge E, Kim J, Sasaki H, Park S, Kryukov G, Garraway L, Hammerman P, Haas J, Andrews S, Lipson D, Stephens P, Miller V, Varella-Garcia M, Janne P, Doebele R
Nat Med, 2013-10-27;19(11):1469-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75NTR-dependent mechanism.
Authors: Howard, Laura, Wyatt, Sean, Nagappan, Guhan, Davies, Alun M
Development, 2013-05-01;140(10):2108-17.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
J Neurosci, 2013-03-20;33(12):5399-410.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Regulation of innate CD8+ T-cell activation mediated by cytokines.
Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Mouse natural killer (NK) cells express the nerve growth factor receptor TrkA, which is dynamically regulated.
Authors: Ralainirina N, Brons NH, Ammerlaan W, Hoffmann C, Hentges F, Zimmer J
PLoS ONE, 2010-12-01;5(12):e15053.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Neurotrophic regulation of fibroblast dedifferentiation during limb skeletal regeneration in the axolotl (Ambystoma mexicanum).
Authors: Satoh A, Cummings GM, Bryant SV, Gardiner DM
Dev. Biol., 2009-11-24;337(2):444-57.
Species: Amphibian
Sample Types: In Vivo
Applications: In Vivo

SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
Development, 2007-09-01;134(18):3327-38.
Species: Mouse, Rat
Sample Types: Whole Cells, Whole Tissue
Applications: Bioassay