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Rat TrkA Antibody

Catalog #: AF1056
43 Citations 2 Reviews Datasheet / COA / SDS
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AF1056
AF1056-SP
Detection of Rat TrkA by Western Blot.
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Product Details
Citations (43)
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Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat TrkA Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TrkA in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human TrkA and less than 1% cross-reactivity with recombinant mouse (rm) TrkB and rmTrkC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TrkA
Ala33-Pro418
Accession # P35739
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat TrkA antibody by Western Blot. View Larger

Detection of Rat TrkA by Western Blot. Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TrkA antibody in Rat Dorsal Root Ganglion by Immunohistochemistry (IHC-Fr). View Larger

TrkA in Rat Dorsal Root Ganglion. TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Detection of Porcine TrkA by Immunohistochemistry View Larger

Detection of Porcine TrkA by Immunohistochemistry Immunohistochemical characterization of ChR2-expressing DRG neurons. A, Immunolabeling of ChR2+ neurons (Venus; green) with either NF200, TrkC, CGRP, TrkA, TRPV1, or IB4 (red) in the L4 DRG contralateral and ipsilateral to PNI on day 14. B, Percentage of colocalization of each marker in ChR2+ DRG neurons [n = 4 rats (two to three slices per rat)]. Size-frequency histogram (inset) illustrating the distribution of the cross-sectional areas of ChR2+ NF200+ DRG neurons in the L4 DRG ipsilateral (371 cells) and contralateral (316 cells) to PNI on day 14. Values represent mean ± SEM. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29468190), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Rat TrkA by Immunohistochemistry View Larger

Detection of Rat TrkA by Immunohistochemistry Immunohistochemical characterization of ChR2-expressing DRG neurons. A, Immunolabeling of ChR2+ neurons (Venus; green) with either NF200, TrkC, CGRP, TrkA, TRPV1, or IB4 (red) in the L4 DRG contralateral and ipsilateral to PNI on day 14. B, Percentage of colocalization of each marker in ChR2+ DRG neurons [n = 4 rats (two to three slices per rat)]. Size-frequency histogram (inset) illustrating the distribution of the cross-sectional areas of ChR2+ NF200+ DRG neurons in the L4 DRG ipsilateral (371 cells) and contralateral (316 cells) to PNI on day 14. Values represent mean ± SEM. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29468190), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4, and BDNF and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat TrkA isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA-I is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the co-expression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

References
  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.
Long Name
Neurotrophic Tyrosine Kinase Receptor A
Entrez Gene IDs
4914 (Human); 18211 (Mouse); 59109 (Rat)
Alternate Names
DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

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Citations for Rat TrkA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Cartilage-binding antibodies induce pain through immune complex–mediated activation of neurons
    Authors: Alex Bersellini Farinotti, Gustaf Wigerblad, Diana Nascimento, Duygu B. Bas, Carlos Morado Urbina, Kutty Selva Nandakumar et al.
    Journal of Experimental Medicine
  2. Dissociation between the relief of skeletal pain behaviors and skin hypersensitivity in a model of bone cancer pain
    Authors: Jean-Marc G. Guedon, Geraldine Longo, Lisa A. Majuta, Michelle L. Thomspon, Michelle N. Fealk, Patrick W. Mantyh
    Pain
  3. Kv7.5 is the primary Kv7 subunit expressed in C-fibers
    Authors: Chih H. King, Steven S. Scherer
    The Journal of Comparative Neurology
  4. Breast Cancer-Induced Bone Remodeling, Skeletal Pain, and Sprouting of Sensory Nerve Fibers
    Authors: Aaron P. Bloom, Juan M. Jimenez-Andrade, Reid N. Taylor, Gabriela Castañeda-Corral, Magdalena J. Kaczmarska, Katie T. Freeman et al.
    The Journal of Pain
  5. Dynamic expression of transcription factor Brn3b during mouse cranial nerve development
    Authors: Szilard Sajgo, Seid Ali, Octavian Popescu, Tudor Constantin Badea
    Journal of Comparative Neurology
  6. The majority of myelinated and unmyelinated sensory nerve fibers that innervate bone express the tropomyosin receptor kinase A
    Authors: Gabriela Castañeda-Corral, Juan M. Jimenez-Andrade, Aaron P. Bloom, Reid N. Taylor, William G. Mantyh, Magdalena J. Kaczmarska et al.
    Neuroscience
  7. Mechanisms of nerve growth factor signaling in bone nociceptors and in an animal model of inflammatory bone pain
    Authors: Sara Nencini, Mitchell Ringuet, Dong-Hyun Kim, Yu-Jen Chen, Claire Greenhill, Jason J Ivanusic
    Mol Pain
  8. c-Maf-positive spinal cord neurons are critical elements of a dorsal horn circuit for mechanical hypersensitivity in neuropathy
    Authors: N Frezel, M Ranucci, E Foster, H Wende, P Pelczar, R Mendes, RP Ganley, K Werynska, S d'Aquin, C Beccarini, C Birchmeier, HU Zeilhofer, H Wildner
    Cell Reports, 2023-03-21;42(4):112295.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain
    Authors: DM Bogdan, K Studholme, A DiBua, C Gordon, MP Kanjiya, M Yu, M Puopolo, M Kaczocha
    Oncogene, 2022-06-02;12(1):9241.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch
    Authors: K Kanehisa, K Koga, S Maejima, Y Shiraishi, K Asai, M Shiratori-, MF Xiao, H Sakamoto, PF Worley, M Tsuda
    Nature Communications, 2022-05-02;13(1):2367.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons
    Authors: S Nencini, M Morgan, J Thai, AI Jobling, SB Mazzone, JJ Ivanusic
    Frontiers in Physiology, 2021-07-15;12(0):644929.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  12. Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis
    Authors: K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato
    Journal of pharmacological sciences, 2021-04-07;146(3):125-135.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  13. Loss of Prdm12 during development, but not in mature nociceptors, causes defects in pain sensation
    Authors: MA Landy, M Goyal, KM Casey, C Liu, HC Lai
    Cell Reports, 2021-03-30;34(13):108913.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
    Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
    Cell Rep, 2020-11-24;33(8):108425.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates
    Authors: L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab
    Nat Commun, 2020-08-21;11(1):4175.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain
    Authors: M Shaqura, L Li, DM Mohamed, X Li, S Treskatsch, C Buhrmann, M Shakibaei, A Beyer, SA Mousa, M Schäfer
    J Neuroinflammation, 2020-06-12;17(1):183.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  17. ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of A&delta and C fiber sensory neurons that innervate bone
    Authors: M Morgan, J Thai, P Trinh, M Habib, KN Effendi, JJ Ivanusic
    Molecular pain, 2020-01-01;16(0):1744806920975.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. A cell fitness selection model for neuronal survival during development
    Authors: Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend
    Nat Commun, 2019-09-12;10(1):4137.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity
    Authors: X Ding, H Wang, X Qian, X Han, L Yang, Y Cao, Q Wang, J Yang
    Cell Rep, 2019-06-25;27(13):3799-3807.e3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
    Authors: S Wiszniak, Q Schwarz
    BMC Neurosci, 2019-04-29;20(1):21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  21. PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis
    Authors: L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast
    Cell Rep, 2019-03-26;26(13):3484-3492.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis
    Authors: H Chen, B Hu, X Lv, S Zhu, G Zhen, M Wan, A Jain, B Gao, Y Chai, M Yang, X Wang, R Deng, L Wang, Y Cao, S Ni, S Liu, W Yuan, H Chen, X Dong, Y Guan, H Yang, X Cao
    Nat Commun, 2019-01-14;10(1):181.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Differential modulation of voltage-gated sodium channels by nerve growth factor in three major subsets of TrkA-expressing nociceptors
    Authors: I Schaefer, V Prato, A Arcourt, FJ Taberner, SG Lechner
    Mol Pain, 2018-11-02;14(0):1744806918814.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Optogenetic Activation of Non-Nociceptive A? Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats
    Authors: R Tashima, K Koga, M Sekine, K Kanehisa, Y Kohro, K Tominaga, K Matsushita, H Tozaki-Sai, Y Fukazawa, K Inoue, H Yawo, H Furue, M Tsuda
    eNeuro, 2018-02-15;5(1):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  25. The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur
    Authors: SR Chartier, SAT Mitchell, LA Majuta, PW Mantyh
    Neuroscience, 2018-02-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  26. [EXPRESS] Immunohistochemical localization of NGF, TrkA and p75 in the bone and articular cartilage of the mouse femur
    Authors: SR Chartier, SA Mitchell, LA Majuta, P Mantyth
    Mol Pain, 2017-11-22;0(0):1744806917745.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway
    Authors: EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze
    J. Neurosci. Methods, 2017-03-07;282(0):34-42.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  28. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2014-11-24;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.
    Authors: Acosta C, Djouhri L, Watkins R, Berry C, Bromage K, Lawson S
    J Neurosci, 2014-01-22;34(4):1494-509.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC
  30. Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain.
    Authors: Evans L, Loescher A, Boissonade F, Whawell S, Robinson P, Andrew D
    BMC Neurosci, 2014-01-01;15(0):1.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
    Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
    J Biol Chem, 2013-03-07;288(16):11144-54.
    Species: Human, Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
    Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR
    J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  33. Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2+ DRG sensory neurons
    Authors: NJ Yang, J Isensee, DV Neel, AU Quadros, HB Zhang, J Lauzadis, SM Liu, S Shiers, A Belu, S Palan, S Marlin, J Maignel, A Kennedy-Cu, VS Tong, M Moayeri, P Röderer, A Nitzsche, M Lu, BL Pentelute, O Brüstle, V Tripathi, KA Foster, TJ Price, RJ Collier, SH Leppla, M Puopolo, BP Bean, TM Cunha, T Hucho, IM Chiu
    Nature Neuroscience, 2021-12-20;0(0):.
  34. Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification
    Authors: Vladimir Vladimirovich Muzyka, Tudor Constantin Badea
    Neural Development
  35. Acute inflammation sensitizes knee-innervating sensory neurons and decreases mouse digging behavior in a TRPV1-dependent manner
    Authors: Sampurna Chakrabarti, Luke A. Pattison, Kaajal Singhal, James R.F. Hockley, Gerard Callejo, Ewan St. John Smith
    Neuropharmacology
  36. Secreted herpes simplex virus-2 glycoprotein G alters thermal pain sensitivity by modifying NGF effects on TRPV1
    Authors: Jorge Rubén Cabrera, Abel Viejo-Borbolla, Antonio Alcamí, Francisco Wandosell
    Journal of Neuroinflammation
  37. A Local Source of FGF Initiates Development of the Unmyelinated Lineage of Sensory Neurons
    Authors: Saïda Hadjab, Marina C. M. Franck, Yiqiao Wang, Ulrich Sterzenbach, Anil Sharma, Patrik Ernfors et al.
    The Journal of Neuroscience
  38. Effects of cerebrolysin on nerve growth factor system in the aging rat brain
    Authors: Mikhail Stepanichev, Mikhail Onufriev, Viktor Aniol, Sofia Freiman, Hemma Brandstaetter, Stefan Winter et al.
    Restorative Neurology and Neuroscience
  39. Sensitization of Cutaneous Primary Afferents in Bone Cancer Revealed by In Vivo Calcium Imaging
    Authors: Larissa de Clauser, Ana P. Luiz, Sonia Santana-Varela, John N. Wood, Shafaq Sikandar
    Cancers (Basel)
  40. Nociceptor subtypes are born continuously over DRG development.
    Authors: Mark A. Landy, Megan Goyal, Helen C. Lai
    Developmental Biology
  41. Preventive or late administration of anti-NGF therapy attenuates tumor-induced nerve sprouting, neuroma formation, and cancer pain
    Authors: Juan Miguel Jimenez-Andrade, Joseph R. Ghilardi, Gabriela Castañeda-Corral, Michael A. Kuskowski, Patrick W. Mantyh
    Pain
  42. Muscle-selective RUNX3 dependence of sensorimotor circuit development
    Authors: Yiqiao Wang, Haohao Wu, Pavel Zelenin, Paula Fontanet, Simone Wanderoy, Charles Petitpré et al.
    Development
  43. Inhibitory Kcnip2 neurons of the spinal dorsal horn control behavioral sensitivity to environmental cold
    Authors: Gioele W. Albisetti, Robert P. Ganley, Francesca Pietrafesa, Karolina Werynska, Marília Magalhaes de Sousa, Rebecca Sipione et al.
    Neuron

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Rat TrkA Antibody
By Anonymous on 10/31/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Bone Extracts Species: Mouse
Immunocytochemistry/Immunofluorescence Rat TrkA Antibody AF1056
Rat TrkA Antibody
By Anonymous on 11/22/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: E12 DRG neurons Species: Mouse

E12 mouse DRG neurons grown overnight with 10ng/ml NGF, then fixed in 4% PFA, blocked for 1 h with 5% BSA, and incubated overnight in 1% BSA containing AF1056 (1:300). A fluorophore-conjugated secondary antibody was used to visualise reactivity.

Immunocytochemistry/Immunofluorescence Rat TrkA Antibody AF1056