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Mouse IL-22 Antibody

Catalog #: AF582
23 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF582
AF582-SP
IL‑10 Secretion Induced by IL‑22 and Neutralization by Mouse IL‑22 Antibody.
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Citations (23)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-22 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-22 in ELISAs and Western blots. In sandwich immunoassays, approximately 75% cross-reactivity with recombinant rat IL‑22 is observed and less than 25% cross-reactivity with recombinant human IL-22 is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑22 (Catalog # 582-ML)

Mouse IL-22 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
1-4 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑22 Biotinylated Antibody (Catalog # BAF582)

Standard: Recombinant Mouse IL-22 Protein (Catalog # 582-ML)

Neutralization
Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑22.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑10 Secretion Induced by IL‑22 and Neutralization by Mouse IL‑22 Antibody. View Larger

IL‑10 Secretion Induced by IL‑22 and Neutralization by Mouse IL‑22 Antibody. Recombinant Mouse IL-22 (Catalog # 582-ML) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Mouse IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582). The ND50 is typically 0.5-2.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

Long Name
Interleukin 22
Entrez Gene IDs
50616 (Human); 50929 (Mouse); 500836 (Rat)
Alternate Names
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

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Citations for Mouse IL-22 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Antibiotics in neonatal life increase murine susceptibility to experimental psoriasis
    Authors: Peter Zanvit, Joanne E. Konkel, Xue Jiao, Shimpei Kasagi, Dunfang Zhang, Ruiqing Wu et al.
    Nature Communications
  2. IL-33 Signaling Regulates Innate IL-17A and IL-22 Production via Suppression of Prostaglandin E2 during Lung Fungal Infection
    Authors: JM Garth, KM Reeder, MS Godwin, JJ Mackel, CW Dunaway, JP Blackburn, C Steele
    J. Immunol., 2017-08-07;199(6):2140-2148.
  3. IL-22 exacerbates the severity of CVB3-induced acute viral myocarditis in IL-17A-deficient mice
    Authors: QING KONG, YIMIN XUE, WEIFENG WU, FAN YANG, YANLI LIU, MENGSHA GAO et al.
    Molecular Medicine Reports
  4. mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy
    Authors: Irene Olivera, Elixabet Bolaños, Jose Gonzalez-Gomariz, Sandra Hervas-Stubbs, Karina V. Mariño, Carlos Luri-Rey et al.
    Cell Reports Medicine
  5. Interleukin-22: implications for liver ischemia-reperfusion injury
    Authors: Paul J. Chestovich, Yoichiro Uchida, William Chang, Mark Ajalat, Charles Lassman, Robert Sabat et al.
    Transplantation
  6. TNF hampers intestinal tissue repair in colitis by restricting IL-22 bioavailability
    Authors: J Ninnemann, C Winsauer, M Bondareva, AA Kühl, L Lozza, P Durek, D Lissner, B Siegmund, SHE Kaufmann, MF Mashreghi, SA Nedospasov, AA Kruglov
    Mucosal Immunology, 2022-04-05;0(0):.
    Species: Mouse
    Sample Types: In-Vivo
  7. Host immunology and rational immunotherapy for carbapenem-resistant Klebsiella pneumoniae infection
    Authors: N Iwanaga, I Sandquist, A Wanek, J McCombs, K Song, JK Kolls
    JCI Insight, 2020-04-23;5(8):.
    Species: Mouse
    Sample Types: In Vivo
  8. Modulation of lymphocyte-mediated tissue repair by rational design of heterocyclic aryl hydrocarbon receptor agonists
    Authors: J Chen, CA Haller, FE Jernigan, SK Koerner, DJ Wong, Y Wang, JE Cheong, R Kosaraju, J Kwan, DD Park, B Thomas, S Bhasin, RC De La Rosa, AM Premji, L Liu, E Park, AC Moss, A Emili, M Bhasin, L Sun, EL Chaikof
    Sci Adv, 2020-01-15;6(3):eaay8230.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Commensal Lactobacillus Controls Immune Tolerance during Acute Liver Injury in Mice
    Authors: N Nakamoto, T Amiya, R Aoki, N Taniki, Y Koda, K Miyamoto, T Teratani, T Suzuki, S Chiba, PS Chu, A Hayashi, A Yamaguchi, S Shiba, R Miyake, T Katayama, W Suda, Y Mikami, N Kamada, H Ebinuma, H Saito, M Hattori, T Kanai
    Cell Rep, 2017-10-31;21(5):1215-1226.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Interleukin-22 level is negatively correlated with neutrophil recruitment in the lungs in a Pseudomonas aeruginosa pneumonia model
    Authors: A Broquet, C Jacqueline, M Davieau, A Besbes, A Roquilly, J Martin, J Caillon, L Dumoutier, JC Renauld, M Heslan, R Josien, K Asehnoune
    Sci Rep, 2017-09-08;7(1):11010.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Expression of IL-22 in the Skin Causes Th2-Biased Immunity, Epidermal Barrier Dysfunction, and Pruritus via Stimulating Epithelial Th2 Cytokines and the GRP Pathway
    Authors: H Lou, J Lu, EB Choi, MH Oh, M Jeong, S Barmettler, Z Zhu, T Zheng
    J. Immunol, 2017-02-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Lacrimal gland-derived IL-22 regulates IL-17-mediated ocular mucosal inflammation
    Authors: YW Ji, SK Mittal, HS Hwang, EJ Chang, JH Lee, Y Seo, A Yeo, H Noh, HS Lee, SK Chauhan, HK Lee
    Mucosal Immunol, 2017-01-04;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Surface-bound bovine serum albumin carrier protein as present in recombinant cytokine preparations amplifies T helper 17 cell polarization
    Sci Rep, 2016-11-03;6(0):36598.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
    Nature, 2016-07-13;535(7612):440-3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2011-10-28;80(1):410-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
    Authors: Li Z, Burns AR, Miller SB, Smith CW
    FASEB J., 2011-04-25;25(8):2659-68.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. STAT3-dependent IL-21 production from T helper cells regulates hematopoietic progenitor cell homeostasis.
    Authors: Kaplan MH, Glosson NL, Stritesky GL, Yeh N, Kinzfogl J, Rohrabaugh SL, Goswami R, Pham D, Levy DE, Brutkiewicz RR, Blum JS, Cooper S, Hangoc G, Broxmeyer HE
    Blood, 2011-04-19;117(23):6198-201.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
    Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
    J. Exp. Med., 2011-03-07;208(3):491-503.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  19. IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).
    Authors: Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW
    PLoS ONE, 2010-01-14;5(1):e8720.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  20. Thymic self-reactivity selects natural interleukin 17-producing T cells that can regulate peripheral inflammation.
    Authors: Marks BR, Nowyhed HN, Choi JY, Poholek AC, Odegard JM, Flavell RA, Craft J
    Nat. Immunol., 2009-09-06;10(10):1125-32.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. IL-22-dependent attenuation of T cell-dependent (ConA) hepatitis in herpes virus entry mediator deficiency.
    Authors: Wahl C, Wegenka UM, Leithauser F, Schirmbeck R, Reimann J
    J. Immunol., 2009-04-15;182(8):4521-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Increased Expressions of IL-22 and Th22 cells in the coxsackievirus B3-Induced mice acute viral myocarditis
    Authors: Qing Kong, Weifeng Wu, Fan Yang, Yanli Liu, Yimin Xue, Mengsha Gao et al.
    Virology Journal
  23. Promoting Effect of L-Fucose on the Regeneration of Intestinal Stem Cells through AHR/IL-22 Pathway of Intestinal Lamina Propria Monocytes
    Authors: Chen Tan, Gaichao Hong, Zhe Wang, Caihan Duan, Lingzhi Hou, Junhao Wu et al.
    Nutrients

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Mouse IL-22 Antibody
By Balaji Mahender on 02/14/2018
Application: ELISA Sample Tested: Citrate Plasma,EDTA Plasma,Heparin Plasma Species: Human