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Recombinant Mouse IL-22 Protein

Catalog #: 582-ML
35 Citations Datasheet / COA / SDS

Carrier Free

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582-ML-010/CF

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582-ML-010
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Citations (35)
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Recombinant Mouse IL-22 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. Nagalakshmi, M.L. et al. (2004) International Immunopharmacology 4:679. The ED50 for this effect is 60-300 pg/mL.
Source
E. coli-derived mouse IL-22 protein
Leu34-Val179, with an N-terminal Met
Accession #
Q9JJY9
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
16.5 kDa

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582-ML (with carrier)

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582-ML/CF (carrier free)

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

582-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

582-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References
  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) PNAS 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Entrez Gene IDs
50616 (Human); 50929 (Mouse); 500836 (Rat)
Alternate Names
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

Citations for Recombinant Mouse IL-22 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Bi-directional signalling between the intestinal epithelium and type-3 innate lymphoid cells (ILC3) regulates secretory dynamics and IL-22
    Authors: Read, E;Peña-Cearra, A;Coman, D;Jowett, GM;W H Chung, M;Coales, I;Syntaka, S;Finlay, RE;Tachó-Piñot, R;van Der Post, S;Naizi, U;Roberts, LB;Hepworth, MR;Curtis, MA;Neves, JF;
    Mucosal immunology
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  2. Liposomal IL-22 ameliorates liver fibrosis through miR-let7a/STAT3 signaling in mice
    Authors: El-Shorbagy, AA;Shafaa, MW;Salah Elbeltagy, R;El-Hennamy, RE;Nady, S;
    International immunopharmacology
    Species: Mouse
    Sample Types: Liposomes, Whole Cells
    Applications: Bioassay
  3. Intermittent fasting promotes ILC3s secreting IL-22 contributing to the beigeing of white adipose tissue
    Authors: Chen, H;Sun, L;Feng, L;Han, X;Zhang, Y;Zhai, W;Zhang, Z;Mulholland, M;Zhang, W;Yin, Y;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Dihydromyricetin Protects Intestinal Barrier Integrity by Promoting IL-22 Expression in ILC3s through the AMPK/SIRT3/STAT3 Signaling Pathway
    Authors: J Zhou, J Yue, Y Yao, P Hou, T Zhang, Q Zhang, L Yi, M Mi
    Nutrients, 2023-01-10;15(2):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. miR-21-3p/IL-22 Axes Are Major Drivers of Psoriasis Pathogenesis by Modulating Keratinocytes Proliferation-Survival Balance and Inflammatory Response
    Authors: F Abdallah, E Henriet, A Suet, A Arar, R Clemençon, JM Malinge, G Lecellier, P Baril, C Pichon
    Cells, 2021-09-26;10(10):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Phospholipase C epsilon mediates cytokine cascade induced by acute disruption of epidermal permeability barrier in mice
    Authors: J Zhang, J Wu, M Sun, S Zhang, J Huang, M Man, L Hu
    Biochemistry and Biophysics Reports, 2020-12-09;24(0):100869.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Modulation of lymphocyte-mediated tissue repair by rational design of heterocyclic aryl hydrocarbon receptor agonists
    Authors: J Chen, CA Haller, FE Jernigan, SK Koerner, DJ Wong, Y Wang, JE Cheong, R Kosaraju, J Kwan, DD Park, B Thomas, S Bhasin, RC De La Rosa, AM Premji, L Liu, E Park, AC Moss, A Emili, M Bhasin, L Sun, EL Chaikof
    Sci Adv, 2020-01-15;6(3):eaay8230.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. T cell deletion of MyD88 connects IL-17 and IkBz to RAS oncogenesis
    Authors: C Cataisson, R Salcedo, AM Michalowsk, M Klosterman, S Naik, L Li, MJ Pan, A Sweet, JQ Chen, LG Kostecka, M Karwan, L Smith, RM Dai, CA Stewart, L Lyakh, WT Hsieh, A Khan, H Yang, M Lee, G Trinchieri, SH Yuspa
    Mol. Cancer Res., 2019-06-04;0(0):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. IL-1 and TNF? Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration
    Authors: H Katsura, Y Kobayashi, PR Tata, BLM Hogan
    Stem Cell Reports, 2019-03-28;0(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  10. Protection against Staphylococcus aureus Colonization and Infection by B- and T-Cell-Mediated Mechanisms
    Authors: F Zhang, O Ledue, M Jun, C Goulart, R Malley, YJ Lu
    MBio, 2018-10-16;9(5):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Interleukin 22 Expands Transit-Amplifying Cells While Depleting Lgr5+ Stem Cells via Inhibition of Wnt and Notch Signaling
    Authors: JM Zha, HS Li, Q Lin, WT Kuo, ZH Jiang, PY Tsai, N Ding, J Wu, SF Xu, YT Wang, J Pan, XM Zhou, K Chen, M Tao, MA Odenwald, A Tamura, S Tsukita, JR Turner, WQ He
    Cell Mol Gastroenterol Hepatol, 2018-09-18;7(2):255-274.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3+ T Cell through Modulation of CD11b+ Cell Function
    Authors: J Wu, J Gu, S Zhou, H Lu, Y Lu, L Lu, X Wang
    J Immunol Res, 2018-08-07;2018(0):1605341.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model
    Authors: B Zwarycz, AD Gracz, KR Rivera, IA Williamson, LA Samsa, J Starmer, MA Daniele, L Salter-Cid, Q Zhao, ST Magness
    Cell Mol Gastroenterol Hepatol, 2018-07-04;7(1):1-17.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  14. Administration of RANKL boosts thymic regeneration upon bone marrow transplantation
    Authors: N Lopes, H Vachon, J Marie, M Irla
    EMBO Mol Med, 2017-06-01;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. Antioxidant axis Nrf2-keap1-ARE in inhibition of alcoholic liver fibrosis by IL-22
    Authors: YH Ni, LJ Huo, TT Li
    World J. Gastroenterol., 2017-03-21;23(11):2002-2011.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
    Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
    Mucosal Immunol, 2017-03-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Intestinal commensal bacteria mediate lung mucosal immunity and promote resistance of newborn mice to infection
    Authors: J Gray, K Oehrle, G Worthen, T Alenghat, J Whitsett, H Deshmukh
    Sci Transl Med, 2017-02-08;9(376):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection
    Sci Rep, 2016-09-21;6(0):32927.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Goblet Cell Derived RELM-beta Recruits CD4+ T Cells during Infectious Colitis to Promote Protective Intestinal Epithelial Cell Proliferation.
    Authors: Bergstrom K, Morampudi V, Chan J, Bhinder G, Lau J, Yang H, Ma C, Huang T, Ryz N, Sham H, Zarepour M, Zaph C, Artis D, Nair M, Vallance B
    PLoS Pathog, 2015-08-18;11(8):e1005108.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Interleukin-22 induces interleukin-18 expression from epithelial cells during intestinal infection.
    Authors: Munoz M, Eidenschenk C, Ota N, Wong K, Lohmann U, Kuhl A, Wang X, Manzanillo P, Li Y, Rutz S, Zheng Y, Diehl L, Kayagaki N, van Lookeren-Campagne M, Liesenfeld O, Heimesaat M, Ouyang W
    Immunity, 2015-02-10;42(2):321-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  21. Interleukin-22 alleviates metabolic disorders and restores mucosal immunity in diabetes.
    Authors: Wang, Xiaoting, Ota, Naruhisa, Manzanillo, Paolo, Kates, Lance, Zavala-Solorio, Jose, Eidenschenk, Celine, Zhang, Juan, Lesch, Justin, Lee, Wyne P, Ross, Jed, Diehl, Lauri, van Bruggen, Nicholas, Kolumam, Ganesh, Ouyang, Wenjun
    Nature, 2014-08-06;514(7521):237-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M.
    Authors: Rabeony H, Petit-Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, Jegou J, Guillet G, Huguier V, Lecron J, Bernard F, Morel F
    PLoS ONE, 2014-07-10;9(7):e101937.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. Paneth cell extrusion and release of antimicrobial products is directly controlled by immune cell-derived IFN-gamma.
    Authors: Farin H, Karthaus W, Kujala P, Rakhshandehroo M, Schwank G, Vries R, Kalkhoven E, Nieuwenhuis E, Clevers H
    J Exp Med, 2014-06-30;211(7):1393-405.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  24. Candida albicans airway exposure primes the lung innate immune response against Pseudomonas aeruginosa infection through innate lymphoid cell recruitment and interleukin-22-associated mucosal response.
    Authors: Mear J, Gosset P, Kipnis E, Faure E, Dessein R, Jawhara S, Fradin C, Faure K, Poulain D, Sendid B, Guery B
    Infect Immun, 2013-10-28;82(1):306-15.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  25. IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection.
    Authors: Turner, Jan-Eric, Stockinger, Brigitta, Helmby, Helena
    PLoS Pathog, 2013-10-10;9(10):e1003698.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  26. Differential induction of antimicrobial REGIII by the intestinal microbiota and Bifidobacterium breve NCC2950.
    Authors: Natividad J, Hayes C, Motta J, Jury J, Galipeau H, Philip V, Garcia-Rodenas C, Kiyama H, Bercik P, Verdu E
    Appl Environ Microbiol, 2013-10-04;79(24):7745-54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
    Authors: Li Z, Burns AR, Miller SB, Smith CW
    FASEB J., 2011-04-25;25(8):2659-68.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  28. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010-09-29;84(24):12703-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  29. IL-1RL2 and its ligands contribute to the cytokine network in psoriasis.
    Authors: Blumberg H, Dinh H, Dean C, Trueblood ES, Bailey K, Shows D, Bhagavathula N, Aslam MN, Varani J, Towne JE, Sims JE
    J. Immunol., 2010-09-10;185(7):4354-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  30. IL-22 defines a novel immune pathway of antifungal resistance.
    Authors: De Luca A, Zelante T, D'Angelo C
    Mucosal Immunol, 2010-05-05;3(4):361-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
    Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
    J. Immunol., 2010-03-24;184(0):5263-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  32. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia.
    Authors: Aujla SJ, Chan YR, Zheng M, Fei M, Askew DJ, Pociask DA, Reinhart TA, McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM, Myerburg MM, Mason CA, Iwakura Y, Kolls JK
    Nat. Med., 2008-02-10;14(3):275-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease.
    Authors: Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R
    J. Immunol., 2007-05-01;178(9):5973-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  34. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis.
    Authors: Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2006-05-01;36(5):1309-23.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  35. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004-08-01;21(2):241-54.
    Species: Human, Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo

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