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Mouse BAFFR/TNFRSF13C Antibody

Catalog #: AF1357
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1357
AF1357-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BAFFR/TNFRSF13C Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BAFF R in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human BAFF R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BAFF R
Ser10-Ala71
Accession # Q9D8D0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse BAFF R/TNFRSF13C Fc Chimera (Catalog # 1357-BR)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 2106-BF) to immobilized Recombinant Mouse BAFF R/TNFRSF13C Fc Chimera (Catalog # 1357-BR) coated at 200 ng/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 

Mouse BAFF R/TNFRSF13C Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse BAFFR/TNFRSF13C Biotinylated Antibody (Catalog # BAF1357)

Standard: Recombinant Mouse BAFFR/TNFRSF13C Fc Chimera Protein, CF (Catalog # 1357-BR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFFR/TNFRSF13C

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. It is produced by macrophages, dendritic cells, and T lymphocytes. BAFF promotes the survival of B cells and is essential for B cell maturation (1 - 4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF-R selectively binds BAFF. Mouse BAFF-R cDNA encodes a 175 amino acid residue (aa) transmembrane protein with a 71 aa extracellular domain, a 21 aa transmembrane domain, and a 83 aa cytoplasmic region. A second isoform of BAFF R that has a 72 aa cytoplamic region can also be produced by alternative splicing. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cells, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells and has profound defects in antibody mediated immune responses. Similarly, A/WySnJ mice that are defective in BAFF R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses.

References
  1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
  2. Mackay, F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
  3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
  4. Thompson, J.S. et al. (2001) Science 14:2108.
Long Name
B cell Activating Factor Receptor
Entrez Gene IDs
115650 (Human); 72049 (Mouse)
Alternate Names
BAFF R; BAFFR; BAFFR;BAFF-R;BROMIX;CD268;CVID4;prolixin;Tumor necrosis factor receptor superfamily member 13C; BR3; CD268; TNFRSF13C

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Citations for Mouse BAFFR/TNFRSF13C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2015-07-03;30(1):163-72.
  2. Neutrophils contribute to excess serum BAFF levels and promote CD4+ T cell and B cell responses in lupus-prone mice.
    Authors: Coquery C, Wade N, Loo W, Kinchen J, Cox K, Jiang C, Tung K, Erickson L
    PLoS ONE, 2014-07-10;9(7):e102284.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
    Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
    J Immunol, 2014-03-19;192(8):3582-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture Treatment
  4. Functional implication of BAFF synthesis and release in gangliosides-stimulated microglia.
    Authors: Kim KS, Park JY, Jou I, Park SM
    J. Leukoc. Biol., 2009-04-30;86(2):349-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer
    Authors: Ding Jianyi, Gan Haili, Yin Bo, Yang Meiqin, Huang Baoyou, Hu Haoran et al.
    Cancer Immunology, Immunotherapy

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