Human SMURF1 Antibody

Catalog #: MAB9507 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9507-100
MAB9507-SP

Detection of Human SMURF1 by Western Blot.

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All SMURF1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SMURF1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SMURF1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 530131

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SMURF1
Met496-Glu757
Accession # Q9HCE7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SMURF1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMURF1 Monoclonal Antibody (Catalog # MAB9507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SMURF1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human SMURF1 by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SMURF1 at approximately 82 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human SMURF1 Monoclonal Antibody (Catalog # MAB9507). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMURF1

Smurf-1 is a 757 amino acids E3 ubiquitin-protein ligase encoded by the SMURF1 gene. Smurf-1 ubiquitinates receptor regulated SMAD proteins acting as a negative regulator of BMP signaling pathway, and it has been shown to inhibit osteoblast differentiation by targeting Runx2, the master regulator of osteoblast differentiation, for degradation.

Long Name

SMAD Ubiquitination Regulatory Factor 1

Entrez Gene IDs

57154 (Human); 75788 (Mouse); 690516 (Rat)

Alternate Names

E3 ubiquitin ligase SMURF1; EC 6.3.2; EC 6.3.2.-; hSMURF1; KIAA1625; KIAA1625E3 ubiquitin-protein ligase SMURF1; SMAD specific E3 ubiquitin protein ligase 1; SMAD ubiquitination regulatory factor 1; Smad-specific E3 ubiquitin ligase 1; SMAD-specific E3 ubiquitin-protein ligase 1; SMURF1