Human Pro-BMP‑2 Antibody

Name: Human Pro-BMP‑2 Antibody
Brand: R&D Systems
SKU: MAB2260
Rating: 5 (1 reviews)

Catalog #: MAB2260
4 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2260
MAB2260-SP

All BMP-2 Antibodies

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pro-BMP‑2 Antibody Summary

Species Reactivity

Human

Specificity

Detects the pro region of the human BMP-2 precursor in direct ELISAs and Western blots. This antibody does not recognize mature recombinant human BMP‑2.

Source

Monoclonal Mouse IgG_2A Clone # 253717

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP‑2
Ala22-Lys281 (Arg245Ser, Arg279Ser)
Accession # P12643

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human BMP‑2 (Pro Region)

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2

BMP-2, a member of the transforming growth factor beta (TGF-beta ) superfamily, is synthesized as an inactive precursor protein. The N-terminal pro region is removed via proteolytic cleavage to liberate a free 114 amino acid C-terminal fragment that forms a disulfide-linked active BMP-2 homodimer. BMP-2 modulates growth and differentiation of many cell types through its interaction with heterodimeric receptors composed of BMPR-II complexed with BMPR-IA or -IB.

Long Name

Bone Morphogenetic Protein 2

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Alternate Names

BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Pro-BMP‑2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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