Human/Primate BMP-4 Antibody

Catalog #: MAB7571
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7571-SP
MAB7571-100
MAB7571-500

All BMP-4 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate BMP-4 Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human and primate BMP-4 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) BMP‑2, rhBMP‑3, rhBMP‑5, rhBMP‑6, or rhBMP‑7 under non-reducing conditions.

Source

Monoclonal Mouse IgG_2B Clone # 66110

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP‑4
Ser293-Arg408
Accession # P12644

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human BMP‑4 (Catalog # 314-BP) under non-reducing conditions only

Human/Primate BMP-4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human/Primate BMP‑4 Biotinylated Antibody (Catalog # BAM7572)

Standard: Recombinant Human BMP-4 Protein (Catalog # 314-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

Long Name

Bone Morphogenetic Protein 4

Entrez Gene IDs

652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

Alternate Names

BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Primate BMP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
Blood, 2012-04-03;119(21):5037-47.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: IHC, Immunoprecipitation, Western Blot
Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade.
Authors: Du Y, Xiao Q, Yip HK
Invest. Ophthalmol. Vis. Sci., 2010-02-03;51(7):3764-73.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot
Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
J. Biol. Chem., 2008-03-13;283(20):13874-88.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
Authors: Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
Cancer Res., 2005-09-15;65(18):8274-85.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot