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Human PREB Antibody

Catalog #: AF5557
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5557
AF5557-SP
Detection of Human PREB by Western Blot.
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Citations (2)
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Human PREB Antibody Summary

Species Reactivity
Human
Specificity
Detects human PREB in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PREB
Met1-Ser388
Accession # Q9HCU5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PREB antibody by Western Blot. View Larger

Detection of Human PREB by Western Blot. Western blot shows lysates of human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5557) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PREB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PREB

PREB (Prolactin regulatory element-binding protein; also mSEC12) is a 45 kDa member of the WD-repeat protein family. Although it is ubiquitously expressed, its expression level varies, tissue-to-tissue. It apparently acts as both a DNA-binding transcription factor, and a catalyst for vesicle formation from the ER. Human PREB is a 417 amino acid (aa) type II transmembrane protein. It is found in the ER and nuclear envelope, and may undergo splicing to generate a transcriptional factor. PREB contains a large cytoplasmic region (aa 1‑388) that displays three WD repeats (aa 152‑337), and an eight aa lumenal domain (aa 410‑417). The transcription factor region lies between aa 175‑417. Multiple splice forms exist. There is an alternate start site at Met73 that may be accompanied by either a 47 aa substitution for aa 388‑417, or a 14 aa substitution for aa 409‑417. Over aa 1‑388, human PREB shares 88% aa identity with mouse PREB.

Long Name
Prolactin Regulatory Element Binding
Entrez Gene IDs
10113 (Human); 50907 (Mouse)
Alternate Names
MGC3467; PREB; prolactin regulatory binding-element protein; prolactin regulatory element binding; prolactin regulatory element-binding protein; SEC12; SEC12Mammalian guanine nucleotide exchange factor mSec12

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Citations for Human PREB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The ER-Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis.
    Authors: Ge L, Melville D, Zhang M, Schekman R
    Elife, 2013-08-06;2(0):e00947.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Sec12 binds to Sec16 at transitional ER sites.
    Authors: Montegna EA, Bhave M, Liu Y
    PLoS ONE, 2012-02-08;7(2):e31156.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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