Human GDNF Quantikine ELISA Kit Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
4.7 - 300 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human GDNF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human GDNF Immunoassay is a 4.0 hour solid phase ELISA designed to measure human GDNF levels in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant human GDNF and antibodies raised against the recombinant protein. Results obtained for naturally occurring human GDNF showed linear curves that were parallel to the standard curves obtained using the Quantikine® Human GDNF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human GDNF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess
intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess
inter-assay precision. Assays were performed by at least three technicians
Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, Plasma, EDTA Plasma, Heparin Plasma
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean (pg/mL) |
30.1 |
92.2 |
183 |
29.7 |
88.2 |
178 |
| Standard Deviation |
0.727 |
1.38 |
3.4 |
1.82 |
5.16 |
10.3 |
| CV% |
2.4 |
1.5 |
1.9 |
6.1 |
5.8 |
5.9 |
Recovery
The recovery of human GDNF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
102 |
85-112 |
| Cell Lysis Buffer (n=4) |
99 |
93-107 |
| EDTA Plasma (n=5) |
81 |
65-91 |
| Heparin Plasma (n=5) |
84 |
69-95 |
| Serum (n=5) |
86 |
72-99 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human GDNF were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Human GDNF Standard Curve
Product Datasheets
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Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: GDNF
GDNF (Glial Cell Line-derived Neurotrophic Factor) is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. It is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF binding to GFR alpha 1 induces the recruitment of Ret, NCAM-1/CD56, various integrins, Syndecan-3, or N-Cadherin. GDNF-based therapies show promise in several neurodegenerative disorders, particularly Parkinson’s disease.
Long Name:
Glial Cell line-derived Growth Factor
Entrez Gene IDs:
2668 (Human); 25453 (Rat)
Alternate Names:
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3
