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Human GDNF Biotinylated Antibody

Catalog #: BAF212
9 Citations Datasheet / COA / SDS
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BAF212
Product Details
Citations (9)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GDNF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GDNF in ELISAs and Western blots. In sandwich immunoassays, less than 30% cross-reactivity with recombinant rat GDNF is observed and less than 0.1% cross-reativity with recombinant human (rh) BDNF, rhCNTF, rhNeurturin, rh beta -NGF, rhNT-3, and rhNT-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF
Arg109-Ile211
Accession # P39905
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GDNF (Catalog # 212-GD)
Recombinant Rat GDNF (Catalog # 512-GF)

Human GDNF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human GDNF Antibody (Catalog # MAB212)

Capture Reagent: Human GDNF Antibody (Catalog # MAB212R)

Standard: Recombinant Human GDNF Protein (Catalog # 212-GD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name
Glial Cell line-derived Growth Factor
Entrez Gene IDs
2668 (Human); 25453 (Rat)
Alternate Names
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3

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Citations for Human GDNF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Intermittent convection-enhanced delivery of GDNF into rhesus monkey putamen: absence of local or cerebellar toxicity
    Authors: Matthias Luz, Philip C. Allen, John Bringas, Chris Boiko, Diane E. Stockinger, Kristen J. Nikula et al.
    Archives of Toxicology
  2. Sleeping Beauty transposon system for GDNF overexpression of entrapped stem cells in fibrin hydrogel in a rat model of Parkinson’s disease
    Authors: Laura Stahn, Justyna Rasińska, Tilo Dehne, Stefanie Schreyer, Aileen Hakus, Manfred Gossen et al.
    Drug Delivery and Translational Research
  3. Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats
    Authors: Geneviève Gowing, Brandon Shelley, Kevin Staggenborg, Amanda Hurley, Pablo Avalos, Jesse Victoroff et al.
    NeuroReport
  4. A regulatable AAV vector mediating GDNF biological effects at clinically-approved sub-antimicrobial doxycycline doses
    Authors: Abdelwahed Chtarto, Marie Humbert-Claude, Olivier Bockstael, Atze T Das, Sébastien Boutry, Ludivine S Breger et al.
    Molecular Therapy - Methods & Clinical Development
  5. Transplantation of human neural progenitor cells secreting GDNF into the spinal cord of patients with ALS: a phase 1/2a trial
    Authors: RH Baloh, JP Johnson, P Avalos, P Allred, S Svendsen, G Gowing, K Roxas, A Wu, B Donahue, S Osborne, G Lawless, B Shelley, K Wheeler, C Prina, D Fine, T Kendra-Rom, H Stokes, V Manoukian, A Muthukumar, L Garcia, MG Bañuelos, M Godoy, C Bresee, H Yu, D Drazin, L Ross, R Naruse, H Babu, EA Macklin, A Vo, A Elsayegh, W Tourtellot, M Maya, M Burford, F Diaz, CG Patil, RA Lewis, CN Svendsen
    Nature Medicine, 2022-09-05;28(9):1813-1822.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. Intravitreal Co-Administration of GDNF and CNTF Confers Synergistic and Long-Lasting Protection against Injury-Induced Cell Death of Retinal Ganglion Cells in Mice
    Authors: S Dulz, M Bassal, K Flachsbart, K Riecken, B Fehse, S Schlichtin, S Bartsch, U Bartsch
    Cells, 2020-09-11;9(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. AAV2-mediated gene transfer of GDNF to the striatum of MPTP monkeys enhances the survival and outgrowth of co-implanted fetal dopamine neurons.
    Authors: Elsworth JD, Redmond DE, Leranth C, Bjugstad KB, Sladek JR, Collier TJ, Foti SB, Samulski RJ, Vives KP, Roth RH
    Exp. Neurol., 2008-02-15;211(1):252-8.
    Species: Primate - Chlorocebus sabaeus (Green Monkey)
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Transient striatal delivery of GDNF via encapsulated cells leads to sustained behavioral improvement in a bilateral model of Parkinson disease.
    Authors: Sajadi A, Bensadoun JC, Schneider BL, Lo Bianco C, Aebischer P
    Neurobiol. Dis., 2005-11-21;22(1):119-29.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Oxidative stress induced by sustained supraphysiological intrastriatal GDNF delivery is prevented by dose regulation
    Authors: Marcelo Duarte Azevedo, Naika Prince, Marie Humbert-Claude, Virginia Mesa-Infante, Cheryl Jeanneret, Valentine Golzne et al.
    Mol Ther Methods Clin Dev

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