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Human GDF-11/BMP-11 Antibody

Catalog #: MAB19581
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB19581
MAB19581-SP
GDF‑11/BMP‑11 in Human Colon Cancer Tissue.
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Citations (19)
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Human GDF-11/BMP-11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GDF-11/BMP-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BMP-6 or recombinant mouse GDF-8 is observed.
Source
Monoclonal Mouse IgG1 Clone # 743833
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GDF-11/BMP-11
Asn299-Ser407
Accession # O95390
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GDF-11/BMP-11 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

GDF‑11/BMP‑11 in Human Colon Cancer Tissue. GDF-11/BMP-11 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human GDF-11/BMP-11 Monoclonal Antibody (Catalog # MAB19581) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to epithelia of the colon. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-11/BMP-11

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide which contains a signal sequence for secretion and an RXXR proteolytic processing site to yield a 109 aa residue carboxy-terminal mature protein (1). Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99‑100% identical. GDF-11 and GDF-8 share 90% amino acid sequence identity within the mature protein. As detected by in situ hybridization, GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1, 2). Likewise, a targeted deletion of GDF-11 in mice results in a spectrum of abnormalities including palatal malformation, vertebral defects, elongated trunks with a reduced or absent tail, missing or malformed kidneys, and an increased number of neurons in the olfactory epithelium (2-5). An intriguing finding in the knockout mice was that the trunk elongation was due to an increase in the number of thoracic vertebrae (4). This implicates GDF-11 as the first secreted factor to influence the specification of segmental identity in vertebrates (3). In fact, GDF-11 does regulate expression of segmental transcription factors, the Hox genes (6). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (7). Despite the strong expression in the limb throughout development, no limb abnormalities were found in the knockout mice. However, in vitro micromass studies indicate that GDF-11 inhibits myogenic and chondrogenic cell differentiation and may impact formation and development of the limb skeleton (6).

References
  1. Gamer, L.W. et al. (1999) Dev. Biol. 208: 222. 
  2. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
  3. Gad, J.M. and P.P.L. Tam (1999) Curr. Biol. 9:R783.  
  4. McPherron, A.C. et al. (1999) Nat. Genet. 22:260.
  5. Esquela, A.F. and S.J. Lee (2003) Dev. Biol. 257:356.
  6. Gamer, L.W. et al. (2001) Dev. Biol. 229:407.
  7. Oh, S.P. et al. (2002) Genes & Dev. 16:274.
Long Name
Growth Differentiation Factor 11
Entrez Gene IDs
10220 (Human)
Alternate Names
BMP11; BMP-11; BMP-11BMP11Bone morphogenetic protein 11; GDF11; GDF-11; growth differentiation factor 11; growth/differentiation factor 11

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Citations for Human GDF-11/BMP-11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease
    Authors: Marissa J Schafer
    Cell Metab, 2016-06-14;23(6):1207-15.
  2. Acute endurance exercise modulates growth differentiation factor 11 in cerebrospinal fluid of healthy young adults
    Authors: Martin Schön, Karin Marček Malenovská, Michal Nemec, Nikoleta Alchus Laiferová, Igor Straka, Zuzana Košutzká et al.
    Front Endocrinol (Lausanne)
  3. GDF11 promotes wound healing in diabetic mice via stimulating HIF-1?-VEGF/SDF-1?-mediated endothelial progenitor cell mobilization and neovascularization
    Authors: Y Zhang, YY Zhang, ZW Pan, QQ Li, LH Sun, X Li, MY Gong, XW Yang, YY Wang, HD Li, LN Xuan, YC Shao, MM Li, MY Zhang, Q Yu, Z Li, XF Zhang, DH Liu, YM Zhu, ZY Tan, YY Zhang, YQ Liu, Y Zhang, L Jiao, BF Yang
    Acta pharmacologica Sinica, 2022-11-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy
    Authors: J Sun, Y Li, X Yang, W Dong, J Yang, Q Hu, C Zhang, H Fang, A Liu
    Aging Cell, 2021-12-14;0(0):e13532.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. GDF11 inhibits cardiomyocyte pyroptosis and exerts cardioprotection in acute myocardial infarction mice by upregulation of transcription factor HOXA3
    Authors: Z Li, H Xu, X Liu, Y Hong, H Lou, H Liu, X Bai, L Wang, X Li, SM Monayo, JN Mokembo, NK Jha, B Yang, Y Zhang
    Cell Death Dis, 2020-10-25;11(10):917.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Endogenous GDF11 regulates odontogenic differentiation of dental pulp stem cells
    Authors: X Qi, Q Xiao, R Sheng, S Jiang, Q Yuan, W Liu
    J. Cell. Mol. Med., 2020-08-26;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Prostate tumor-derived GDF11 accelerates androgen deprivation therapy-induced sarcopenia
    Authors: C Pan, N Jaiswal Ag, Y Zulia, S Singh, K Sha, JL Mohler, KH Eng, J Chakkalaka, JJ Krolewski, KL Nastiuk
    JCI Insight, 2020-03-26;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Mutations in GDF11 and the extracellular antagonist, Follistatin, as a likely cause of Mendelian forms of orofacial clefting in humans
    Authors: Timothy C. Cox, Andrew C. Lidral, Jason C. McCoy, Huan Liu, Liza L. Cox, Ying Zhu et al.
    Human Mutation
  9. Effects of Exercise Training on Growth and Differentiation Factor 11 Expression in Aged Mice
    Authors: Minjung Lee, Satoshi Oikawa, Takashi Ushida, Katsuhiko Suzuki, Takayuki Akimoto
    Frontiers in Physiology
  10. A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice
    Authors: Q Jin, C Qiao, J Li, B Xiao, J Li, X Xiao
    Skelet Muscle, 2019-05-27;9(1):16.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice
    Authors: C Ozek, RC Krolewski, SM Buchanan, LL Rubin
    Sci Rep, 2018-11-23;8(1):17293.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  12. Neonatal Systemic AAV-Mediated Gene Delivery of GDF11 Inhibits Skeletal Muscle Growth
    Authors: Q Jin, C Qiao, J Li, J Li, X Xiao
    Mol. Ther., 2018-02-02;26(4):1109-1117.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  13. Modulation of GDF11 expression and synaptic plasticity by age and training
    Authors: E De Domenic, G D'Arcangel, I Faraoni, M Palmieri, V Tancredi, G Graziani, P Grimaldi, L Tentori
    Oncotarget, 2017-08-03;8(35):57991-58002.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Exogenous GDF11 induces cardiac and skeletal muscle dysfunction and wasting
    Authors: Teresa A. Zimmers, Yanling Jiang, Meijing Wang, Tiffany W. Liang, Joseph E. Rupert, Ernie D. Au et al.
    Basic Research in Cardiology
  15. Activin A more prominently regulates muscle mass in primates than does GDF8
    Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
    Nat Commun, 2017-04-28;8(0):15153.
    Species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Rat
    Sample Types: In Vivo, Serum
    Applications: ELISA Development, Neutralization
  16. GDF11 Attenuates Development of Type 2 Diabetes via Improvement of Islet ? cell Function and Survival
    Authors: H Li, Y Li, L Xiang, J Zhang, B Zhu, L Xiang, J Dong, M Liu, G Xiang
    Diabetes, 2017-04-27;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Supraphysiological levels of GDF11 induce striated muscle atrophy
    Authors: DW Hammers, M Merscham-B, JY Hsiao, S Engst, JJ Hartman, HL Sweeney
    EMBO Mol Med, 2017-04-01;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  18. The Immateriality of Circulating GDF11
    Authors: Buel D. Rodgers
    Circulation Research
  19. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer
    Authors: Bajikar SS, Wang CC, Borten MA et al.
    Dev. Cell.

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