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Human Free BDNF ELISA Kit - Quantikine

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Human Free BDNF ELISA Cell Culture Supernate Standard Curve
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Summary Sample Values Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Free BDNF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Citrate Plasma (50 uL)
Sensitivity
20 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human BDNF
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human free BDNF in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum* (n=33)27,7931006186-42,580
Platelet-poor EDTA plasma (n=33)42856ND-4137
Platelet-poor heparin plasma (n=33)19655ND-528
Platelet-poor citrate plasma (n=33)40194ND-1838
*Samples were diluted prior to assay.
 ND=Non-detectable

Cell Culture Supernates - Human peripheral blood  mononuclear cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate and stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed on days 1 and 5 and assayed for levels of human free BDNF.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
Unstimulated132109
Stimulated160104



Product Summary

The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.

Recovery

The recovery of BDNF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 96 83-105
Platelet-poor Citrate Plasma (n=5) 99 87-120
Platelet-poor EDTA Plasma (n=5) 96 89-115
Platelet-poor Heparin Plasma (n=5) 93 81-119
Serum (n=5) 100 85-116

Linearity

To assess the linearity of the assay, five samples containing or spiked with high concentrations of BDNF in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Free BDNF ELISA Linearity

Scientific Data

N/A Human Free BDNF ELISA Cell Culture Supernate Standard Curve View Larger

Human Free BDNF ELISA Cell Culture Supernate Standard Curve

N/A Human Free BDNF ELISA Serum/Plasma Standard Curve View Larger

Human Free BDNF ELISA Serum/Plasma Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Long Name:
Brain-derived Neurotrophic Factor
Entrez Gene IDs:
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names:
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    Go to the next step. Do not wash.
    5. 100 µL Conjugate
  5.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  6.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    7. 200 µL Substrate Solution
  7.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    8. 50 µL Stop Solution
  8.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Free BDNF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

104 Citations: Showing 1 - 10
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  1. Relationship between inflammatory markers in human olfactory neural progenitor cells and antidepressant response
    Authors: Mónica, FR;Gerardo Bernabé, RR;Rodrigo, GZ;Melissa, SB;Lorena, RB;
    Journal of psychiatric research
    Species: Human
    Sample Types: Serum, Cell Culture Supernates
  2. The Effects of Polyphenol Supplementation on BDNF, Cytokines and Cognition in Trained Male Cyclists following Acute Ozone Exposure during High-Intensity Cycling
    Authors: Morton, L;Paton, C;Braakhuis, A;
    Nutrients
    Species: Human
    Sample Types: Serum
  3. Reduced Levels of Lacrimal Glial Cell Line-Derived Neurotrophic Factor (GDNF) in Patients with Focal Epilepsy and Focal Epilepsy with Comorbid Depression: A Biomarker Candidate
    Authors: Shpak, AA;Rider, FK;Druzhkova, TA;Zhanina, MY;Popova, SB;Guekht, AB;Gulyaeva, NV;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  4. Stress-induced red nucleus attenuation induces anxiety-like behavior and lymph node CCL5 secretion
    Authors: Shi, DD;Zhang, YD;Zhang, S;Liao, BB;Chu, MY;Su, S;Zhuo, K;Hu, H;Zhang, C;Wang, Z;
    Nature communications
    Species: Human
    Sample Types: Plasma
  5. Elevated Plasma Levels of Mature Brain-Derived Neurotrophic Factor in Major Depressive Disorder Patients with Higher Suicidal Ideation
    Authors: Li, H;Zhao, M;Jiang, C;Zhao, H;Wu, C;Li, Y;Zhang, S;Xu, P;Mou, T;Xu, Y;Huang, M;
    Brain sciences
    Species: Human
    Sample Types: Plasma
  6. The role of acute changes in mBDNF, cortisol and pro-BDNF in predicting cognitive performance in old age
    Authors: Nilsson, J;Ekblom, M;Moberg, M;Lövdén, M;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  7. Association between Psychopathological Symptoms and Aggression and Selected Biochemical Parameters in Adolescents with Behavioural and Emotional Disturbances
    Authors: M ?miarowska, M Pawlicka, A Boro?, A Grzywacz, K Safranow, D Chlubek, V Dziedziejk
    International Journal of Molecular Sciences, 2023-04-12;24(8):.
    Species: Human
    Sample Types: Plasma
  8. Blood-Brain Barrier Biomarkers before and after Kidney Transplantation
    Authors: L Hernandez, LJ Ward, S Arefin, P Barany, L Wennberg, M Söderberg, S Bruno, V Cantaluppi, P Stenvinkel, K Kublickien
    International Journal of Molecular Sciences, 2023-04-01;24(7):.
    Species: Human
    Sample Types: Plasma
  9. Relationship between Brain-Derived Neurotrophic Factor and Cognitive Decline in Patients with Mild Cognitive Impairment and Dementia
    Authors: M Nikolac Pe, F Borovecki, I Filipcic, B Vuic, T Milos, G Nedic Erja, M Konjevod, L Tudor, N Mimica, S Uzun, O Kozumplik, D Svob Strac, N Pivac
    Biomolecules, 2023-03-21;13(3):.
    Species: Human
    Sample Types: Plasma
  10. Multidomain cognitive training increases physical activity in people with Parkinson's disease with mild cognitive impairment
    Authors: M Bode, P Sulzer, C Schulte, S Becker, K Brockmann, S Elben, AK Folkerts, A Ophey, C Schlensted, K Witt, L Wojtecki, J Evers, W Maetzler, E Kalbe, I Liepelt-Sc
    Parkinsonism & related disorders, 2023-02-17;0(0):105330.
    Species: Human
    Sample Types: Serum
  11. Brain-derived neurotrophic factor associated with kidney function
    Authors: CY Hsu, WH Sheu, IT Lee
    Diabetology & metabolic syndrome, 2023-02-13;15(1):16.
    Species: Human
    Sample Types: Serum
  12. C5L2 modulates BDNF production in human dental pulp stem cells via p38alpha pathway
    Authors: M Irfan, S Chung
    Scientific Reports, 2023-01-02;13(1):74.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Preliminary results of the cross-sectional associations of sedentary behavior and physical activity with serum brain-derived neurotrophic factor in adults with coronary heart disease
    Authors: A Ullrich, K Wenzel, M Bahls, L Voigt, S Könemann, M Dörr, S Wurm, S Ulbricht
    Scientific Reports, 2022-11-16;12(1):19685.
    Species: Human
    Sample Types: Serum
  14. Multi-Strain Probiotic Mixture Affects Brain Morphology and Resting State Brain Function in Healthy Subjects: An RCT
    Authors: J Rode, HMT Edebol Car, J König, AN Hutchinson, P Thunberg, J Persson, RJ Brummer
    Cells, 2022-09-19;11(18):.
    Species: Human
    Sample Types: Serum
  15. Impact of Freeze-Drying on Cord Blood (CB), Serum (S), and Platelet-Rich Plasma (CB-PRP) Preparations on Growth Factor Content and In Vitro Cell Wound Healing
    Authors: V Sabrina, C Carmen, A Gloria, B Elisa, C Nico, B Marina, F Luigi, V Piera
    International Journal of Molecular Sciences, 2022-09-14;23(18):.
    Species: Human
    Sample Types: Serum
  16. Acute Normobaric Hypoxia Lowers Executive Functions among Young Men despite Increase of BDNF Concentration
    Authors: M Chroboczek, S Kujach, M ?uszczyk, T Grzywacz, H Soya, R Laskowski
    International Journal of Environmental Research and Public Health, 2022-08-30;19(17):.
    Species: Human
    Sample Types: Serum
  17. Judo training program improves brain and muscle function and elevates the peripheral BDNF concentration among the elderly
    Authors: S Kujach, M Chroboczek, J Jaworska, A Sawicka, M Smaruj, P Winklewski, R Laskowski
    Scientific Reports, 2022-08-16;12(1):13900.
    Species: Human
    Sample Types: Serum
  18. A Novel Cre/lox71-Based System for Inducible Expression of Recombinant Proteins and Genome Editing
    Authors: M Karagyaur, D Dyikanov, P Tyurin-Kuz, S Dzhauari, M Skryabina, M Vigovskiy, A Primak, N Kalinina, V Tkachuk
    Cells, 2022-07-07;11(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Urokinase-Type Plasminogen Activator Enhances the Neuroprotective Activity of Brain-Derived Neurotrophic Factor in a Model of Intracerebral Hemorrhage
    Authors: S Dzhauari, S Litvinova, A Efimenko, N Aleksandru, N Basalova, M Abakumov, N Danilova, P Malkov, V Balabanyan, T Bezuglova, V Balayants, M Mnikhovich, M Gulyaev, M Skryabina, V Popov, D Stambolsky, T Voronina, V Tkachuk, M Karagyaur
    Biomedicines, 2022-06-08;10(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Prospective associations of multimodal serum biomarkers with 12-week and 12-month remission in patients with depressive disorders receiving stepwise psychopharmacotherapy
    Authors: JM Kim, HJ Kang, JW Kim, M Jhon, W Choi, JY Lee, SW Kim, IS Shin, MG Kim, R Stewart
    Brain, Behavior, and Immunity, 2022-05-23;104(0):65-73.
    Species: Human
    Sample Types: Serum
  21. Impact of fasting on stress systems and depressive symptoms in patients with major depressive disorder: a cross-sectional study
    Authors: B Stapel, D Fraccaroll, M Westhoff-B, J Bauersachs, R Lichtingha, K Jahn, A Burkert, V Buchholz, S Bleich, H Frieling, XQ Ding, KG Kahl
    Scientific Reports, 2022-05-10;12(1):7642.
    Species: Human
    Sample Types: Serum
  22. Interaction effects of diabetes and brain-derived neurotrophic factor on suicidal ideation in patients with acute coronary syndrome
    Authors: W Choi, JW Kim, HJ Kang, HK Kim, HC Kang, JY Lee, SW Kim, YJ Hong, Y Ahn, MH Jeong, R Stewart, JM Kim
    Scientific Reports, 2022-04-22;12(1):6602.
    Species: Human
    Sample Types: Serum
  23. The Importance of Endoplasmic Reticulum Stress as a Novel Antidepressant Drug Target and Its Potential Impact on CNS Disorders
    Authors: M Jó?wiak-B?, P Soko?owska, M Siatkowska, CA Panek, P Komorowski, E Kowalczyk, A Wiktorowsk
    Pharmaceutics, 2022-04-12;14(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Blood-brain barrier and gut barrier dysfunction in chronic kidney disease with a focus on circulating biomarkers and tight junction proteins
    Authors: L Hernandez, LJ Ward, S Arefin, T Ebert, A Laucyte-Ci, GOING-FWD, O Heimbürger, P Barany, L Wennberg, P Stenvinkel, K Kublickien
    Scientific Reports, 2022-03-15;12(1):4414.
    Species: Human
    Sample Types: Serum
  25. Methylation Status of Exon IV of the Brain-Derived Neurotrophic Factor (BDNF)-Encoding Gene in Patients with Non-Diabetic Hyperglycaemia (NDH) before and after a Lifestyle Intervention
    Authors: HA Fachim, N Malipatil, K Siddals, R Donn, GY Cortés, CF Dalton, JM Gibson, AH Heald
    Epigenomes, 2022-02-18;6(1):.
    Species: Human
    Sample Types: Serum
  26. Association between brain-derived neurotropic factor (BDNF), high-sensitivity C-reactive protein (hs-CRP) and psychiatric symptoms in medicated and unmedicated patients
    Authors: H Soloey-Nil, K Nygaard-Od, MG Kristianse, OL Brekke, TE Mollnes, SK Reitan, T Oiesvold
    BMC Psychiatry, 2022-02-03;22(1):84.
    Species: Human
    Sample Types: Serum
  27. TGF-beta1 in plasma and cerebrospinal fluid can be used as a biological indicator of chronic pain in patients with osteoarthritis
    Authors: YC Liu, HT Hsiao, JC Wang, TC Wen, SL Chen
    PLoS ONE, 2022-01-21;17(1):e0262074.
    Species: Human
    Sample Types: Plasma
  28. Serum and plasma brain-derived neurotrophic factor concentration are elevated by systemic but not local passive heating
    Authors: T Ogawa, SP Hoekstra, YI Kamijo, VL Goosey-Tol, JJ Walsh, F Tajima F, CA Leicht
    PLoS ONE, 2021-12-09;16(12):e0260775.
    Species: Human
    Sample Types: Serum
  29. Plasma BDNF and Cytokines Correlated with Protein Biomarkers for Bipolar II Disorder
    Authors: SY Lee, TY Wang, RB Lu, LJ Wang, CH Chang, YC Chiang, CC Pan, KW Tsai
    Journal of personalized medicine, 2021-12-02;11(12):.
    Species: Human
    Sample Types: Plasma
  30. Randomized Controlled Trial of a Cognitive Intervention to Improve Memory in Heart Failure
    Authors: SJ Pressler, M Jung, I Gradus-Piz, M Titler, D Smith, S Gao, KR Lake, H Burney, D Clark, K Wierenga, SG Dorsey, B Giordani
    Journal of cardiac failure, 2021-11-08;0(0):.
    Species: Human
    Sample Types: Serum
  31. The contribution of platelets to peripheral BDNF elevation in children with autism spectrum disorder
    Authors: CA Farmer, AE Thurm, B Honnekeri, P Kim, SE Swedo, JC Han
    Scientific Reports, 2021-09-13;11(1):18158.
    Species: Human
    Sample Types: Serum
  32. Tai chi improves psychoemotional state, cognition, and motor learning in older adults during the COVID-19 pandemic
    Authors: R Solianik, D Mickevi?ie, L Žlibinait?, A ?ekanauska
    Experimental gerontology, 2021-04-19;150(0):111363.
    Species: Human
    Sample Types: Serum
  33. Beneficial effects of whole-body cryotherapy on glucose homeostasis and amino acid profile are associated with a reduced myostatin serum concentration
    Authors: M Koz?owska, J Kortas, M ?ychowska, J Antosiewic, K ?uczek, S Perego, G Lombardi, E Ziemann
    Scientific Reports, 2021-03-29;11(1):7097.
    Species: Human
    Sample Types: Serum
  34. Reinforcement of Self-Regulated Brain Activity in Schizophrenia Patients Undergoing Rehabilitation
    Authors: R Markiewicz, B Dobrowolsk
    BioMed Research International, 2021-03-26;2021(0):8030485.
    Species: Human
    Sample Types: Serum
  35. Kinetics of serum brain-derived neurotrophic factor (BDNF) concentration levels in epileptic patients after generalized tonic-clonic seizures
    Authors: ?A Poniatowsk, A Cudna, K Kurczych, E Bronisz, I Kurkowska-
    Epilepsy research, 2021-03-11;173(0):106612.
    Species: Human
    Sample Types: Serum
  36. Peripheral BDNF correlated with miRNA in BD-II patients
    Authors: SY Lee, TY Wang, RB Lu, LJ Wang, CH Chang, YC Chiang, KW Tsai
    Journal of psychiatric research, 2021-02-13;136(0):184-189.
    Species: Human
    Sample Types: Plasma
  37. Is Serum BDNF Altered in Acute, Short- and Long-Term Recovered Restrictive Type Anorexia Nervosa?
    Authors: JL Steinhäuse, JA King, FI Tam, M Seidel, R Biemann, ML Wronski, D Geisler, V Roessner, S Ehrlich
    Nutrients, 2021-01-29;13(2):.
    Species: Human
    Sample Types: Serum
  38. Circulating neurotrophins and hemostatic risk factors of atherothrombotic cardiovascular disease at baseline and during sympathetic challenge: the SABPA study
    Authors: R von Känel, M Hamer, A Wentzel, L Malan
    Scientific Reports, 2021-01-27;11(1):2297.
    Species: Human
    Sample Types: Serum
  39. The BDNF rs6265 Polymorphism is a Modifier of Cardiomyocyte Contractility and Dilated Cardiomyopathy
    Authors: FJ Raucci, AP Singh, J Soslow, LW Markham, L Zhong, W Aljafar, N Lessiohadi, CP Awgulewits, P Umbarkar, Q Zhang, PL Cannon, M Buchowski, JT Roland, EJ Carrier, WB Burnette, AK Hatzopoulo, H Lal, CL Galindo
    Int J Mol Sci, 2020-10-10;21(20):.
    Species: Human
    Sample Types: Plasma
  40. BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma
    Authors: M Tylicka, E Matuszczak, A Hermanowic, W D?bek, M Karpi?ska, J Kami?ska, OM Koper-Lenk
    Brain sciences, 2020-09-24;10(10):.
    Species: Human
    Sample Types: Plasma
  41. Exercise-Induced Elevated BDNF Level Does Not Prevent Cognitive Impairment Due to Acute Exposure to Moderate Hypoxia in Well-Trained Athletes
    Authors: Z Piotrowicz, M Chalimoniu, K P?oszczyca, M Czuba, J Langfort
    Int J Mol Sci, 2020-08-04;21(15):.
    Species: Human
    Sample Types: Serum
  42. Physical exercise interventions have no effect on serum BDNF concentration in older adults living in long-term nursing homes
    Authors: C Rezola-Par, G Hervás, H Arrieta, AH Diego, F Ruiz-Litag, SM Gil, A Rodriguez-, J Irazusta
    Exp. Gerontol., 2020-07-18;139(0):111024.
    Species: Human
    Sample Types: Serum
  43. Lack of antidepressant effects of burst-suppressing isoflurane anesthesia in adult male Wistar outbred rats subjected to chronic mild stress
    Authors: W Theilmann, M Rosenholm, P Hampel, W Löscher, T Rantamäki
    PLoS ONE, 2020-06-24;15(6):e0235046.
    Species: Rat
    Sample Types: Tissue Homogenates
  44. A 10-week yoga practice has no effect on cognition, but improves balance and motor learning by attenuating brain-derived neurotrophic factor levels in older adults
    Authors: A ?ekanauska, A Skurvydas, L Žlibinait?, D Mickevi?ie, S Kilikevi?i, R Solianik
    Exp. Gerontol., 2020-06-13;138(0):110998.
    Species: Human
    Sample Types: Serum
  45. Tyrosine triple mutated AAV2-BDNF gene therapy in an inner retinal injury model induced by intravitreal injection of N-methyl-D-aspartate (NMDA)
    Authors: AL Shiozawa, T Igarashi, M Kobayashi, K Nakamoto, S Kameya, S Fujishita, H Takahashi, T Okada
    Mol. Vis., 2020-06-03;26(0):409-422.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
  46. Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedonia
    Authors: C Wu, J Lu, S Lu, M Huang, Y Xu
    J Psychiatr Res, 2020-05-12;126(0):92-97.
    Species: Human
    Sample Types: Plasma
  47. Tai Chi is an effective form of exercise to reduce markers of frailty in older age
    Authors: NF Kasim, JV van Zanten, S Aldred
    Exp. Gerontol., 2020-03-14;135(0):110925.
    Species: Human
    Sample Types: Plasma
  48. Acute increases in brain-derived neurotrophic factor in plasma following physical exercise relates to subsequent learning in older adults
    Authors: J Nilsson, Ö Ekblom, M Ekblom, A Lebedev, O Tarassova, M Moberg, M Lövdén
    Sci Rep, 2020-03-10;10(1):4395.
    Species: Human
    Sample Types: Plasma
  49. Assessing BDNF as a mediator of the effects of exercise on depression
    Authors: KL Szuhany, MW Otto
    J Psychiatr Res, 2020-02-08;123(0):114-118.
    Species: Human
    Sample Types: Serum
  50. Hypoxic Preconditioning Enhances Survival and Proangiogenic Capacity of Human First Trimester Chorionic Villus-Derived Mesenchymal Stem Cells for Fetal Tissue Engineering
    Authors: D Hao, C He, B Ma, L Lankford, L Reynaga, DL Farmer, F Guo, A Wang
    Stem Cells Int, 2019-11-12;2019(0):9695239.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. The impact of physical exercise on cognitive and affective functions and serum levels of brain-derived neurotrophic factor in nursing home residents: A randomized controlled trial
    Authors: H Arrieta, C Rezola-Par, M Kortajaren, G Hervás, J Gil, JJ Yanguas, M Iturburu, SM Gil, J Irazusta, A Rodriguez-
    Maturitas, 2019-11-05;131(0):72-77.
    Species: Human
    Sample Types: Serum
  52. Altered temporal sensitivity in obesity is linked to pro-inflammatory state
    Authors: F Scarpina, P Marzullo, S Mai, A Mauro, M Scacchi, M Costantini
    Sci Rep, 2019-10-29;9(1):15508.
    Species: Human
    Sample Types: Serum
  53. Sex-Specific Associations of Brain-Derived Neurotrophic Factor and Cardiorespiratory Fitness in the General Population
    Authors: ML Schmalhofe, MRP Markus, JC Gras, J Kopp, D Janowitz, HJ Grabe, S Gro beta, R Ewert, S Gläser, D Albrecht, I Eiffler, H Völzke, N Friedrich, M Nauck, A Steveling, S Könemann, K Wenzel, SB Felix, M Dörr, M Bahls
    Biomolecules, 2019-10-20;9(10):.
    Species: Human
    Sample Types: Serum
  54. Preclinical Evaluation of Long-Term Neuroprotective Effects of BDNF-Engineered Mesenchymal Stromal Cells as Intravitreal Therapy for Chronic Retinal Degeneration in Rd6 Mutant Mice
    Authors: R Lejkowska, MP Kawa, E Pius-Sadow, D Rogi?ska, K ?uczkowska, B Machali?sk, A Machali?sk
    Int J Mol Sci, 2019-02-12;20(3):.
    Species: Transgenic Mouse
    Sample Types: Cell Culture Supernates
  55. Comparison between Polybutylcyanoacrylate Nanoparticles with Either Surface-Adsorbed or Encapsulated Brain-Derived Neurotrophic Factor on the Neural Differentiation of iPSCs
    Authors: MH Lin, CY Chung, KT Chen, JC Yeh, TH Lee, MH Lee, IN Lee, WC Huang, JT Yang
    Int J Mol Sci, 2019-01-06;20(1):.
  56. A 2-Week Specific Volleyball Training Supported by the Whole Body Cryostimulation Protocol Induced an Increase of Growth Factors and Counteracted Deterioration of Physical Performance
    Authors: J Jaworska, K Micielska, M Koz?owska, K Wnorowski, J Skrobecki, L Radzimi?sk, A Babi?ska, E Rodziewicz, G Lombardi, E Ziemann
    Front Physiol, 2018-11-28;9(0):1711.
    Species: Human
    Sample Types: Serum
  57. Adipose Tissue-Derived Biomarkers of Intestinal Barrier Functions for the Characterization of Diarrhoea-Predominant IBS
    Authors: F Russo, G Chimienti, G Riezzo, M Linsalata, B D'Attoma, C Clemente, A Orlando
    Dis. Markers, 2018-11-28;2018(0):1827937.
    Species: Human
    Sample Types: Serum
  58. Effects of aerobic training, resistance training, or both on brain-derived neurotrophic factor in adolescents with obesity: The hearty randomized controlled trial
    Authors: Gary S Goldfield, Glen P Kenny, Denis Prud'homme, Martin Holcik, Angela S Alberga, Margaret Fahnestock, Jameason D Cameron, Steve Doucette, Stasia Hadjiyannakis, Heather Tulloch, Mark S Tremblay, Jeremy Walsh, Eva Guerin, Katie E Gunnell, Amedeo D'Angiulli, Ronald J Sigal
    Physiol Behav
    Species: Human
    Sample Types: Serum
  59. A single exercise bout and locomotor learning after stroke: physiological, behavioral, and computational outcomes
    Authors: CC Charalambo, CC Alcantara, MA French, X Li, KS Matt, HE Kim, SM Morton, DS Reisman
    J. Physiol. (Lond.), 2018-04-17;0(0):.
    Species: Human
    Sample Types: Serum
  60. Changes in fatigue, autonomic functions, and blood biomarkers due to sitting isometric yoga in patients with chronic fatigue syndrome
    Authors: T Oka, T Tanahashi, N Sudo, B Lkhagvasur, Y Yamada
    Biopsychosoc Med, 2018-04-10;12(0):3.
    Species: Human
    Sample Types: Plasma
  61. Engineered 3D vascular and neuronal networks in a microfluidic platform
    Authors: T Osaki, V Sivathanu, RD Kamm
    Sci Rep, 2018-03-26;8(1):5168.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Physical Exercise and Spatial Training: A Longitudinal Study of Effects on Cognition, Growth Factors, and Hippocampal Plasticity
    Authors: L Woost, PL Bazin, M Taubert, R Trampel, CL Tardif, A Garthe, G Kempermann, U Renner, G Stalla, DVM Ott, V Rjosk, H Obrig, A Villringer, E Roggenhofe, TA Klein
    Sci Rep, 2018-03-09;8(1):4239.
    Species: Human
    Sample Types: Serum
  63. Effects and mechanism of the HECT study (hybrid exercise-cognitive trainings) in mild ischemic stroke with cognitive decline: fMRI for brain plasticity, biomarker and behavioral analysis
    Authors: TT Yeh, KC Chang, CY Wu, YY Lee, PY Chen, JW Hung
    Contemp Clin Trials Commun, 2018-02-17;9(0):164-171.
    Species: Human
    Sample Types: Serum
  64. Improvement of cognitive functions in response to a regular Nordic walking training in elderly women - A change dependent on the training experience
    Authors: A Gmi?t, J Jaworska, K Micielska, J Kortas, K Prusik, K Prusik, M Lipowski, A Radulska, N Szupryczy?, J Antosiewic, E Ziemann
    Exp. Gerontol., 2018-02-09;104(0):105-112.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  65. Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic stroke
    Authors: YR Kim, SM Ahn, ME Pak, HJ Lee, DH Jung, YI Shin, HK Shin, BT Choi
    Sci Rep, 2018-02-01;8(1):2044.
    Species: Mouse
    Sample Types: Tissue Homogenates
  66. Serum nerve growth factor beta, brain- and glial-derived neurotrophic factor levels and psychopathology in unmedicated patients with schizophrenia
    Authors: CS Chu, CL Chu, CC Wu, T Lu
    J Chin Med Assoc, 2018-02-01;0(0):.
    Species: Human
    Sample Types: Serum
  67. A noradrenergic lesion aggravates the effects of systemic inflammation on the hippocampus of aged rats
    Authors: KL Bharani, R Derex, AC Granholm, A Ledreux
    PLoS ONE, 2017-12-19;12(12):e0189821.
    Species: Rat
    Sample Types: Serum
  68. Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
    Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
    Sci Rep, 2017-07-31;7(1):6871.
    Species: Human
    Sample Types: Serum
  69. Obesity promotes oxidative stress and exacerbates blood-brain barrier disruption after high-intensity exercise
    Authors: Hee-Tae Roh, Su-Youn Cho, and Wi-Young So
    J Sport Health Sci
    Species: Human
    Sample Types: Serum
  70. BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults
    Authors: MS Rahman, V Millischer, Z Zeebari, Y Forsell, C Lavebratt
    J Psychiatr Res, 2017-05-22;93(0):50-58.
    Species: Human
    Sample Types: Serum
  71. The Effects of Taekwondo Training on Peripheral Neuroplasticity-Related Growth Factors, Cerebral Blood Flow Velocity, and Cognitive Functions in Healthy Children: A Randomized Controlled Trial
    Authors: SY Cho, WY So, HT Roh
    Int J Environ Res Public Health, 2017-04-25;14(5):.
    Species: Human
    Sample Types: Serum
  72. The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
    Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
    Transl Psychiatry, 2017-04-04;7(4):e1079.
    Species: Human
    Sample Types: Serum
  73. Affective alterations in patients with Cushing's syndrome in remission are associated with decreased BDNF and cortisone levels
    Authors: E Valassi
    Eur. J. Endocrinol, 2017-02-01;176(2):221-231.
    Species: Human
    Sample Types: Serum
  74. Cross-sectional associations of objectively-measured physical activity with brain-derived neurotrophic factor in adolescents
    Authors: Tao Huang
    Physiol. Behav, 2016-12-24;0(0):.
    Species: Human
    Sample Types: Serum
  75. Stress-induced change in serum BDNF is related to quantitative family history of alcohol use disorder and age at first alcohol use
    Authors: Shobhit Sharma
    Pharmacol. Biochem. Behav, 2016-12-07;153(0):12-17.
    Species: Human
    Sample Types: Serum
  76. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
    Stem Cells Int, 2016-10-20;2016(0):6104780.
    Species: Human
    Sample Types: Cell Culture Supernates
  77. Paracrine Mechanisms of Intravenous Bone Marrow-Derived Mononuclear Stem Cells in Chronic Ischemic Stroke
    Authors: Rohit Bhatia
    Cerebrovasc Dis Extra, 2016-10-19;6(3):107-119.
    Species: Human
    Sample Types: Serum
  78. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016-07-04;6(0):29029.
    Species: Human
    Sample Types: Cell Culture Supernates
  79. Exploring the Association between Serum BDNF and Attempted Suicide
    Sci Rep, 2016-04-28;6(0):25229.
    Species: Human
    Sample Types: Serum
  80. Serum brain-derived neurotrophic factor levels and personality traits in patients with major depression.
    Authors: Nomoto H, Baba H, Satomura E, Maeshima H, Takebayashi N, Namekawa Y, Suzuki T, Arai H
    BMC Psychiatry, 2015-03-04;15(0):33.
    Species: Human
    Sample Types: Serum
  81. Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
    Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
    PLoS ONE, 2015-02-06;10(2):e0116256.
    Species: Human
    Sample Types: Serum
  82. The BDNF Val66Met polymorphism and plasma brain-derived neurotrophic factor levels in Han Chinese heroin-dependent patients.
    Authors: Chen, Shiou-La, Lee, Sheng-Yu, Chang, Yun-Hsua, Wang, Tzu-Yun, Chen, Shih-Hen, Chu, Chun-Hsi, Chen, Po See, Yang, Yen Kuan, Hong, Jau-Shyo, Lu, Ru-Band
    Sci Rep, 2015-02-02;5(0):8148.
    Species: Human
    Sample Types: Plasma
  83. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
    Authors: Chou M, Burnouf T, Wang T
    PLoS ONE, 2014-06-19;9(6):e99145.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. Serum BDNF levels in relation to illness severity, suicide attempts, and central serotonin activity in patients with major depressive disorder: a pilot study.
    Authors: Park Y, Lee B, Um T, Kim S
    PLoS ONE, 2014-03-24;9(3):e91061.
    Species: Human
    Sample Types: Serum
  85. A longitudinal study of alterations of hippocampal volumes and serum BDNF levels in association to atypical antipsychotics in a sample of first-episode patients with schizophrenia.
    Authors: Rizos E, Papathanasiou M, Michalopoulou P, Laskos E, Mazioti A, Kastania A, Vasilopoulou K, Nikolaidou P, Margaritis D, Papageorgiou C, Liappas I
    PLoS ONE, 2014-02-13;9(2):e87997.
    Species: Human
    Sample Types: Serum
  86. Measurement of the levels of leptin, BDNF associated with polymorphisms LEP G2548A, LEPR Gln223Arg and BDNF Val66Met in Thai with metabolic syndrome.
    Authors: Suriyaprom, Kanjana, Tungtrongchitr, Rungsunn, Thawnasom, Kittisak
    Diabetol Metab Syndr, 2014-01-21;6(1):6.
    Species: Human
    Sample Types: Serum
  87. Brain-derived neurotrophic factor in human subjects with function-altering melanocortin-4 receptor variants.
    Authors: Hohenadel M, Thearle M, Grice B, Huang H, Dai M, Tao Y, Hunter L, Palaguachi G, Mou Z, Kim R, Tsang M, Haack K, Voruganti V, Cole S, Butte N, Comuzzie A, Muller Y, Baier L, Krakoff J, Knowler W, Yanovski J, Han J
    Int J Obes (Lond), 2013-11-26;38(8):1068-74.
    Species: Human
    Sample Types: Serum
  88. Bortezomib-induced peripheral neuropathy is related to altered levels of brain-derived neurotrophic factor in the peripheral blood of patients with multiple myeloma.
    Authors: Azoulay, David, Lavie, David, Horowitz, Netanel, Suriu, Celia, Gatt, Moshe E, Akria, Luiza, Perlman, Riki, Braester, Andrei, Ben-Yehuda, Dina
    Br J Haematol, 2013-10-25;164(3):454-6.
    Species: Human
    Sample Types: Serum
  89. A randomized controlled trial of multicomponent exercise in older adults with mild cognitive impairment.
    Authors: Suzuki T, Shimada H, Makizako H, Doi T, Yoshida D, Ito K, Shimokata H, Washimi Y, Endo H, Kato T
    PLoS ONE, 2013-04-09;8(4):e61483.
    Species: Human
    Sample Types: Serum
  90. Daily serum and salivary BDNF levels correlate with morning-evening personality type in women and are affected by light therapy.
    Authors: Tirassa P, Iannitelli A, Sornelli F, Cirulli F, Mazza M, Calza A, Alleva E, Branchi I, Aloe L, Bersani G, Pacitti F
    Riv Psichiatr, 2012-11-01;47(6):527-34.
    Species: Human
    Sample Types: Saliva
  91. Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria.
    Authors: Rossing K, Novak N, Mommert S, Pfab F, Gehring M, Wedi B, Kapp A, Raap U
    Clin. Exp. Allergy, 2011-06-16;41(10):1392-1399.
    Species: Human
    Sample Types: Serum
  92. CD34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans.
    Authors: Sobrino T, Arias S, Perez-Mato M, Agulla J, Brea D, Rodriguez-Yanez M, Castillo J
    J. Neurosci. Res., 2011-04-12;89(7):979-85.
    Species: Human
    Sample Types: Serum
  93. Exercise training increases size of hippocampus and improves memory.
    Authors: Erickson KI, Voss MW, Prakash RS
    Proc. Natl. Acad. Sci. U.S.A., 2011-01-31;108(7):3017-22.
    Species: Human
    Sample Types: Serum
  94. BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
    Int. J. Oncol., 2010-06-01;36(6):1469-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  95. Endurance training enhances BDNF release from the human brain.
    Authors: Seifert T, Brassard P, Wissenberg M, Rasmussen P, Nordby P, Stallknecht B, Adser H, Jakobsen AH, Pilegaard H, Nielsen HB, Secher NH
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009-11-18;298(2):R372-7.
    Species: Human
    Sample Types: Plasma
  96. Androgen regulates brain-derived neurotrophic factor in spinal motoneurons and their target musculature.
    Authors: Verhovshek T, Cai Y, Osborne MC, Sengelaub DR
    Endocrinology, 2009-10-30;151(1):253-61.
    Species: Rat
    Sample Types: Tissue Homogenates
  97. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
    Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
    Cancer Res., 2008-12-01;68(23):9996-10003.
    Species: Human
    Sample Types: Serum
  98. Spinal adenosine A2a receptor activation elicits long-lasting phrenic motor facilitation.
    Authors: Golder FJ, Ranganathan L, Satriotomo I, Hoffman M, Lovett-Barr MR, Watters JJ, Baker-Herman TL, Mitchell GS
    J. Neurosci., 2008-02-27;28(9):2033-42.
    Species: Rat
    Sample Types: Tissue Homogenates
  99. Decreased brain-derived neurotrophic factor (BDNF)- and beta-thromboglobulin (beta-TG)- blood levels in Alzheimer's disease.
    Authors: Laske C, Stransky E, Leyhe T, Eschweiler GW, Schott K, Langer H, Gawaz M
    Thromb. Haemost., 2006-07-01;96(1):102-3.
    Species: Human
    Sample Types: Serum
  100. Transcranial magnetic stimulation and BDNF plasma levels in amyotrophic lateral sclerosis.
    Authors: Angelucci F, Oliviero A, Pilato F, Saturno E, Dileone M, Versace V, Musumeci G, Batocchi AP, Tonali PA, Di Lazzaro V
    Neuroreport, 2004-03-22;15(4):717-20.
    Species: Human
    Sample Types: Serum
  101. Perinatal changes of brain-derived neurotrophic factor in pre- and fullterm neonates.
    Authors: Malamitsi-Puchner A, Economou E, Rigopoulou O, Boutsikou T
    Early Hum. Dev., 2004-01-01;76(1):17-22.
    Species: Human
    Sample Types: Serum
  102. Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury.
    Authors: Mahmood A, Lu D, Chopp M
    J. Neurotrauma, 2004-01-01;21(1):33-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  103. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
    Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
    Exp. Neurol., 2002-06-01;175(0):318.
    Species: Rat
    Sample Types: Cell Culture Supernates
  104. Changes in Circulating BDNF in relation to Sex, Diet, and Exercise: A 12-Week Randomized Controlled Study in Overweight and Obese Participants
    Authors: M. Glud, T. Christiansen, L. H. Larsen, B. Richelsen, and J. M. Bruun
    J Obes
    Species: Human
    Sample Types: Serum

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Reviews for Human Free BDNF Quantikine ELISA Kit

Average Rating: 4.7 (Based on 6 Reviews)

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Human Free BDNF Quantikine ELISA Kit
By Anonymous on 11/15/2023
Sample Tested: Cell culture supernatant
Human Free BDNF Quantikine ELISA Kit DBD00
Human Free BDNF Quantikine ELISA Kit
By Anonymous on 11/12/2020
Sample Tested: Serum and Plasma

Very good standard curve, samplesl worked at a dilution of 1:200

Human Free BDNF Quantikine ELISA Kit DBD00
Human Free BDNF Quantikine ELISA Kit
By Anonymous on 10/24/2020
Sample Tested: EDTA Plasma
Human Free BDNF Quantikine ELISA Kit DBD00
Human Free BDNF Quantikine ELISA Kit
By Ruby Argumedo on 12/05/2018
Sample Tested: Heparin Plasma

We use this kit really frequently so a 6 pack of BDNF is very convenient for us to have. Easy to use and to understand. Results are usually really good and reproducible. Would definitely recommend.

Human Free BDNF Quantikine ELISA Kit
By Anonymous on 11/02/2018
Sample Tested: Serum
Human Free BDNF Quantikine ELISA Kit
By Luiz Alexandre on 07/13/2018
Sample Tested: EDTA Plasma,Heparin Plasma,Mouse lymphocytes
Human Free BDNF Quantikine ELISA Kit DBD00