Human FOLR1 Antibody Summary
Arg25-Met233
Accession # P15328
Applications
Human FOLR1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FOLR1 by Western Blot. Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only.
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Detection of FOLR1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins.
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FOLR1 in MCF-7 Human Cell Line. FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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FOLR1 Specificity is Shown by Flow Cytometry in Knockout Cell Line. FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). No staining in the FOLR1 knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
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Detection of Human FOLR1 by Immunocytochemistry/Immunofluorescence (A) Immunofluorescent (left) and flow cytometry (right) detection of the EpCAM expression in MCF7 cells and the FR alpha expression in A2780 cells. EpCAM and FR alpha stained with Alexa Fluor® 488 are green at an excitation of 488 nm, and the nuclei stained with DAPI are blue at an excitation of 405 nm. As a negative control, we used EpCAM to stain Jurkat cells, which are EpCAM negatively expressed, and we used FR alpha to stain A549 cells, which are FR alpha negatively expressed. Histograms of flow cytometric analysis: MCF7 cells (top) were stained with anti-EpCAM antibodies (red) and A2780 cells (bottom) were stained with anti-FR alpha antibodies (red), the negative control was autofluorescent (orange), and the isotype was mouse IgG plus secondary antibody (blue). (B) Schematic of our CTCs enrichment strategy. Anti-EpCAM-MNs and Anti-FR alpha -MNs was added to the whole blood and after incubation, magnetic separation and fluorescence identification, the cells of DAPI+/CK+/CD45− were defined as CTC. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29352248), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of FOLR1 in HeLa cells by Flow Cytometry HeLa cells were stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOLR1
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).
- Kelemen, L.E. (2006) Int. J. Cancer 119:243.
- Fowler, B. et al. (2001) Kidney Int. 59:S-221.
- Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
- Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
- Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
- Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
- Ross, J.F. et al. (1994) Cancer 73:2432.
- Parker, N. et al. (2005) Anal. Biochem. 338:284.
- Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
- Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
- Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
Product Datasheets
Citations for Human FOLR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Safety and anti-tumour activity of the IgE antibody MOv18 in patients with advanced solid tumours expressing folate receptor-alpha: a phase I trial
Authors: Spicer, J;Basu, B;Montes, A;Banerji, U;Kristeleit, R;Miller, R;Veal, GJ;Corrigan, CJ;Till, SJ;Figini, M;Canevari, S;Barton, C;Jones, P;Mellor, S;Carroll, S;Selkirk, C;Nintos, G;Kwatra, V;Funingana, IG;Doherty, G;Gould, HJ;Pellizzari, G;Nakamura, M;Ilieva, KM;Khiabany, A;Stavraka, C;Chauhan, J;Gillett, C;Pinder, S;Bax, HJ;Josephs, DH;Karagiannis, SN;
Nature communications
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Identifying high-grade serous ovarian carcinoma-specific extracellular vesicles by polyketone-coated nanowires
Authors: Yokoi, A;Ukai, M;Yasui, T;Inokuma, Y;Hyeon-Deuk, K;Matsuzaki, J;Yoshida, K;Kitagawa, M;Chattrairat, K;Iida, M;Shimada, T;Manabe, Y;Chang, IY;Asano-Inami, E;Koya, Y;Nawa, A;Nakamura, K;Kiyono, T;Kato, T;Hirakawa, A;Yoshioka, Y;Ochiya, T;Hasegawa, T;Baba, Y;Yamamoto, Y;Kajiyama, H;
Science advances
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Folate receptor alpha in ovarian cancer tissue and patient serum is associated with disease burden and treatment outcomes
Authors: HJ Bax, J Chauhan, C Stavraka, A Santaolall, G Osborn, A Khiabany, M Grandits, J López-Aben, LCGF Palhares, C Chan Wah H, A Robinson, A Pope, N Woodman, C Naceur-Lom, S Malas, JEM Coumbe, M Nakamura, R Laddach, S Mele, S Crescioli, AM Black, S Lombardi, S Canevari, M Figini, A Sayasneh, S Tsoka, K FitzGerald, C Gillett, S Pinder, M Van Hemelr, R Kristeleit, S Ghosh, A Montes, J Spicer, SN Karagianni, DH Josephs
British Journal of Cancer, 2022-11-19;0(0):.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays
Authors: J Song, LJ Sokoll, DW Chan, Z Zhang
Biomedicines, 2021-12-14;9(12):.
Species: Human
Sample Types: Serum
Applications: ELISA Capture, ELISA Detection -
Efficient delivery of small interfering RNAs targeting particular mRNAs into pancreatic cancer cells inhibits invasiveness and metastasis of pancreatic tumors
Authors: K Taniuchi, T Yawata, M Tsuboi, T Ueba, T Saibara
Oncotarget, 2019-04-23;10(30):2869-2886.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Promising Nanocarriers for PEDF Gene Targeting Delivery to Cervical Cancer Cells Mediated by the Over-expressing FR?
Sci Rep, 2016-08-31;6(0):32427.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Conditions associated with circulating tumor-associated folate receptor 1 protein in healthy men and women.
Authors: Kelemen L, Brenton J, Parkinson C, C Whitaker H, Piskorz A, Csizmadi I, Robson P
PLoS ONE, 2014-05-08;9(5):e96542.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Capture) -
Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity.
Authors: Rudman SM, Josephs DH, Cambrook H, Karagiannis P, Gilbert AE, Dodev T, Hunt J, Koers A, Montes A, Taams L, Canevari S, Figini M, Blower PJ, Beavil AJ, Nicodemus CF, Corrigan C, Kaye SB, Nestle FO, Gould HJ, Spicer JF, Karagiannis SN
Clin. Exp. Allergy, 2011-05-16;41(10):1400-1413.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Unexpected PD-L1 immune evasion mechanism in TNBC, ovarian, and other solid tumors by DR5 agonist antibodies
Authors: T Mondal, GN Shivange, RG Tihagam, E Lyerly, M Battista, D Talwar, R Mosavian, K Urbanek, NS Rashid, JC Harrell, PD Bos, EB Stelow, MS Stack, S Bhatnagar, J Tushir-Sin
Embo Molecular Medicine, 2021-02-15;13(3):e12716.
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Leukemia-specific delivery of mutant NOTCH1 targeted therapy
Authors: Giovanni Roti, Jun Qi, Samuel Kitara, Marta Sanchez-Martin, Amy Saur Conway, Anthony C. Varca et al.
Journal of Experimental Medicine
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Combined use of EpCAM and FR alpha enables the high-efficiency capture of circulating tumor cells in non-small cell lung cancer
Authors: Luojun Chen, Min Peng, Na Li, Qibin Song, Yi Yao, Bin Xu et al.
Scientific Reports
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Circulating Tumor Cell Detection In Epithelial Ovarian Cancer Using Dual-Component Antibodies Targeting EpCAM And FR alpha
Authors: Na Li, Hao Zuo, Luojun Chen, Huali Liu, Jin Zhou, Yi Yao et al.
Cancer Management and Research
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After very long exposure gave a band of an expected size. Very weak signal even with ECL Plus.
R&D Systems Technical Service will investigate.
