Human BMP-2 Biotinylated Antibody

Catalog #: BAM3552 Datasheet / COA / SDS

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BAM3552

All BMP-2 Antibodies

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Human BMP-2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP-2 in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant human (rh) BMP‑4, rhBMP‑2/BMP‑7 heterodimer, and no cross-reactivity with rhBMP-3, -5, -6, -7, rhTGF-beta 1, rhTGF-beta 2, or rhTGF-beta 3 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 100230

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP-2
Gln283-Arg396
Accession # P12643

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human BMP-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human BMP‑2 Antibody (Catalog # MAB3551)

Standard: Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR-IA, or BMPR-IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).

References

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