Chicken Caronte Antibody

Catalog #: MAB1051 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1051
MAB1051-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Chicken Caronte Antibody Summary

Species Reactivity

Chicken

Specificity

Detects chicken Caronte in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cerberus, rhDAN, or recombinant mouse Gremlin is observed.

Source

Monoclonal Mouse IgG₁ Clone # 156808

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant chicken Caronte
Asp16-Asn272
Accession # NP_990154

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Chicken Caronte Fc Chimera (Catalog # 1051-CA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caronte

Chicken Caronte, the ortholog of mammalian Cerberus, is a member of the Cerberus/DAN family of secreted glycoproteins. There are at least 5 Dan Domain (DAND) family members including DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), Cerberus (DAND4), and Coco (DAND5). Caronte is expressed in the left paraxial mesoderm and is the molecule responsible for transducing left-sided positional information from the node to the periphery of the lateral plate mesoderm (LPM) during chicken embryonic development. Caronte functions as a BMP antagonist, inducing Nodal and downstream genes in the LPM by interfering with the repressive activity of bilaterally-produced BMPs. In addition, antagonism of BMPs by Caronte induces the expression of the Lefty gene in the midline. Caronte binds both BMP-4 and BMP-7 in immunoprecipitation experiments. Recombinant chicken Caronte preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and induce heart reversals in Xenopus embryos.

References

Pearce, J. et al. (1999) Dev. Biol. 209:98.
Yokouchi, Y. et al. (1999) Cell. 98:573.
King, T. and N. Brown (1999) Nature 401:222.
Ahu, L. et al. (1999) Curr. Biol. 9:931.
Rodriguez-Esteban, C. et al. (1999) Nature 401:243.

Entrez Gene IDs

395623 (Chicken)

Alternate Names

Caronte; Chicken Caronte

Product Datasheets

Product Datasheet

COA

SDS

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