This protocol outlines a method for the production of R-Spondin 1-conditioned medium from the Cultrex® HA-R-Spondin1-Fc293T Cell Line. Some specific tips and considerations you can find in this protocol include:
- Method for the production of R Spondin 1-conditioned medium in less than 20 days
- Verification of R-Spondin 1 medium by Western blot analysis
- Use of TOPFlash Assay for the monitoring of R-Spondin 1 in cell culture media
