This protocol outlines a method for culturing normal human intestinal organoids based on protocols described by C. Pleguezuelos-Manzano and Hans Clevers (1). It is intended to culture organoids from normal human intestinal tissues using Cultrex™ UltiMatrix RGF Basement Membrane Extract as a scaffold.
This video explains how Cultrex UltiMatrix BME from R&D Systems optimizes clarity, strength, protein content and concentration to provide the ultimate basement membrane matrix for cell culture.
This protocol outlines a method for culturing normal human liver organoids based on protocols described by Meritxell Huch (1). Starting with a suspension of isolated and dissociated human liver tissue, the reagents and
This protocol outlines a method for culturing normal human lung organoids based on protocols described by Norman Sachs and Hans Clevers (1). The procedure that is described allows for the long-term
This protocol outlines a method for the production of R-Spondin 1-conditioned medium from the Cultrex® HA-R-Spondin1-Fc293T Cell Line. Some specific tips and considerations you can find in this protocol include:
This protocol outlines a method for the harvesting of organoids from culture as well as the processing recommendations for passaging and biochemical analysis using the Cultrex® Organoid Harvesting Solution. Some specific tips and considerations you can find in this protocol include:
This protocol outlines a method for preparation of embedding organoids in Cultrex® Basement Membrane Extract (BME) and further analysis of the organoids by immunofluorescence. Some specific tips and considerations you can find in this protocol include:
This protocol outlines a method for the culture of normal human gastric organoids using Cultrex® Organoid Qualified Basement Membrane Extracts (BME) as a scaffold. Some specific tips and considerations you can find in this protocol include:
This protocol outlines a method for the culture of organoid progenitor cells from normal and healthy mouse gastric, small intestine, or colon tissues using Cultrex® Organoid Qualified Basement Membrane Extract as a scaffold. Some specific tips and considerations you can find in this protocol include:
This protocol outlines methods and tips to improve mouse xenograft studies using Cultrex® Basement Membrane Extract (BME). Some specific tips and considerations you can find in this protocol include: