This video protocol provides step-by-step guide on how to perform flow cytometry using antibodies from R&D Systems and Novus Biologicals to stain membrane-associated proteins in suspended cells. This protocol has been developed and optimized by R&D Systems Flow Cytometry Laboratory.
- 0:40 Introduction
- 1:00 Before We Begin
- 1:10 Sample Preparation
- 2:15 Experimental Setup
- 2:30 Cell Preparation
- 2:50 FC Blocking
- 3:25 Adding Conjugated Antibody
- 3:50 Wash Step
- 5:05 Resuspending Cells
- 5:20 Conclusion
View full written flow cytometry protocol for membrane staining
Other Flow Cytometry Staining Protocols
Intracellular Staining Flow Cytometry Protocol using Detergents to Permeabilize the Cells
Intracellular Staining Flow Cytometry Protocol Using Alcohol to Permeabilize the Cells
Propidium Iodide Cell Viability Flow Cytometry Protocol
7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol